Synthesis, characterization, and in vitro evaluation of a radio-metal organic framework composed of in vivo generator 166Dy/166Ho and DOTMP as a novel agent for bone marrow ablation

Sara Vosoghi; Simindokht Shirvani-Arani; Ali Bahrami-Samani; Nafise Salek; Ehsan Mirerezaei; Amir Reza Jalilian

doi:10.1515/ract-2015-2528

Article

Synthesis, characterization, and in vitro evaluation of a radio-metal organic framework composed of in vivo generator ¹⁶⁶Dy/¹⁶⁶Ho and DOTMP as a novel agent for bone marrow ablation

Sara Vosoghi , Simindokht Shirvani-Arani , Ali Bahrami-Samani , Nafise Salek , Ehsan Mirerezaei and Amir Reza Jalilian

Published/Copyright: June 8, 2016

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From the journal Radiochimica Acta Volume 104 Issue 10

Abstract

In this study, the MOF concept contributed to the preparation of a novel, bone-ablating agent composed of Cu(II) using the in vivo generator ¹⁶⁶Dy/¹⁶⁶Ho and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetramethylene phosphonic acid (DOTMP). The product was characterized (IR, elemental analysis: CHN, ICM-MS, PIXE, DLS, XRD) and quality-controlled (radio-thin layer chromatography (RTLC)). The stability and in vitro hydroxyapatite binding was checked up to one week at 37°C in human serum. Radio-MOF crystals and colloidal radio-MOF particles were obtained by varying the synthesizing conditions (including pH and temperature), and they had similar IR patterns and similar elemental analysis results. The estimated chemical formula was [1Lu-1Cu-1Cl-1DOTMP] for both. The final product was synthesized at pH = 8 while stirring at room temperature using ¹⁶⁶Dy/¹⁶⁶Ho-nitrate, CuCl₂, and DOTMP (yield > 99%, RTLC). Dynamic light scattering (DLS) measurements showed particles in the size range of 60 to 100 nm for the resultant radio-MOF particles (RMP). In vitro binding experiments showed acceptable bone-seeking affinity of the prepared formula even after one week of storage in human serum at 37°C. Importantly, this is the first study of the use of the MOF concept to provide a highly-stable organometallic compound containing ¹⁶⁶Dy/¹⁶⁶Ho in vivo generator for bone marrow ablation.

Keywords: MOF; 166Dy/166Ho in vivo generator; DOTMP; bone marrow ablation

Acknowledgement:

The authors are grateful for support of this study by Nuclear Science and Technology Research Institute.

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Received: 2015-10-10

Accepted: 2016-4-26

Published Online: 2016-6-8

Published in Print: 2016-10-1

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https://doi.org/10.1515/ract-2015-2528

Keywords for this article

MOF; 166Dy/166Ho in vivo generator; DOTMP; bone marrow ablation