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Laser photobiomodulation influences the expression of genes related to the inflammatory process and muscle cell differentiation during the process of muscle healing

Einfluss der Laser-Photobiomodulation auf die Genexpression als Reaktion auf Entzündungsprozesse und Muskelzelldifferenzierung während der Muskelheilung
  • Natalia Camargo Rodrigues EMAIL logo , Roberta Brunelli , Heloísa Selistre de Araújo , Nivaldo Antonio Parizotto and Ana Claudia Muniz Renno
Published/Copyright: November 20, 2013
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Photonics & Lasers in Medicine
From the journal Photonics & Lasers in Medicine Volume 3 Issue 1

Abstract

Background and objective: The aim of this study was to evaluate the effects of 780-nm laser therapy on the expression of genes related to muscle healing.

Materials and methods: Sixty-three rats were distributed into three groups: 1) injured control group (CG), 2) injured treatment group at 10 J/cm2 (G10), and 3) injured treatment group at 50 J/cm2 (G50). Histopathological analysis and mRNA genes expression were evaluated after 7, 14 and 21 days post-injury.

Results: At day 7, G10 presented a smaller necrosis area compared to CG and G50. Fourteen days post-surgery, G10 and G50 presented a smaller amount of inflammatory cells and a better tissue organization compared to CG. On day 21, G10 and G50 showed better muscle structure than the control. A significantly decreased COX-2 expression was observed in groups G10 and G50 at day 7 compared to the control animals. No difference was found among the experimental groups after 14 days, but G50 presented statistically higher COX-2 down-regulation at day 21. VEGF expression decreased in the first period analyzed in both treatment groups, increased after 14 days in G10, and increased after 21 days in G50. Both irradiated groups had a higher MyoD expression in all the evaluated periods and myogenin levels increased after 14 days in both treatment groups and in G10 after 21 days.

Conclusion: 780-nm laser therapy had positive effects during muscle regeneration through the gene expression modulation related to the inflammatory process and the new muscle fibers formation, in both fluencies used in the present study, but the fluence of 10 J/cm2 was more efficient.

Zusammenfassung

Hintergrund und Zielsetzung: Das Ziel dieser Studie war es, die Auswirkungen der 780 nm-Laserherapie auf die im Zusammenhang mit der Muskelheilung stehende Genexpression zu bewerten.

Materialien und Methoden: Für die Studie wurden 63 Ratten in drei Gruppen aufgeteilt: 1) verletzte Kontrollgruppe ohne Therapie (CG), 2) verletzte Behandlungsgruppe mit 10 J/cm2 (G10) und 3) verletzte Behandlungsgruppe mit 50 J/cm2 (G50). Die histopathologische Analyse sowie die Bestimmung der Genexpression (mRNA-Expression) erfolgte jeweils 7, 14 und 21 Tage nach der Verletzung.

Ergebnisse: Am Tag 7 wurde in der G10-Gruppe im Vergleich zu den beiden anderen Untersuchungsgruppen CG und G50 ein kleinerer Nekrosebereich festgestellt. Vierzehn Tage nach der Operation zeigten sich in den beiden Behandlungsgruppen G10 und G50 eine kleinere Menge von Entzündungszellen und eine bessere Gewebeorganisation als in der Kontrollgruppe CG. Am Tag 21 erwies sich die Muskelstruktur in den beiden behandelten Gruppen G10 und G50 besser als in der Kontrollgruppe. Nach 7 Tagen wurde im Vergleich zu den Kontrolltieren eine verminderte COX2-Expression in den Gruppen G10 und G50 beobachtet. Es wurde aber kein Unterschied zwischen den experimentellen Gruppen nach 14 Tagen gefunden. Hingegen war die Down-Regulierung der COX2-Expression am Tag 21 in der G50-Gruppe statistisch höher. Die VEGF-Expression sank zunächst in beiden Behandlungsgruppen, erhöhte sich nach 14 Tagen in der G10-Gruppe und nach 21 Tagen in der G50-Gruppe. Beide bestrahlten Gruppen hatten an allen beobachteten Tagen eine höhere MyoD-Expression; der Myogenin-Anteil stieg nach 14 Tagen in beiden behandelten Gruppen an und war nach 21 Tagen in der G10-Gruppe am höchsten.

Fazit: Die 780 nm-Lasertherapie hatte für beide in der Studie untersuchten Bestrahlungsstärken einen positiven Effekt bei der Regeneration der Muskulatur durch die Modulation der Genexpression in Bezug auf die Entzündung und die neue Muskelfaserbildung, allerdings war eine Laserbestrahlung mit 10 J/cm2 effektiver.

Keywords: low level laser therapy; skeletal muscle; muscle healing; cryolesion; Low-Level-Laser-Therapie; Skelettmuskulatur; Muskelheilung; Kryoläsion
Corresponding author: Natalia Camargo Rodrigues, Department of Physiotherapy, Federal University of São Carlos, Rodovia Washington Luís (SP-310), Km 235, Postcode: 13565-905, São Carlos, SP, Brazil, e-mail:

This work was supported by “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior” (CAPES Foundation-20226300).

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Erhalten: 2013-6-19
Revidiert: 2013-7-22
Angenommen: 2013-10-21
Online erschienen: 2013-11-20
Erschienen im Druck: 2014-2-1

©2014 by Walter de Gruyter Berlin Boston

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  5. Announcement
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https://doi.org/10.1515/plm-2013-0039
Keywords for this article
low level laser therapy; skeletal muscle; muscle healing; cryolesion; Low-Level-Laser-Therapie; Skelettmuskulatur; Muskelheilung; Kryoläsion

Articles in the same Issue

  1. Frontmatter
  2. Frontmatter
  3. Editorial
  4. Clinical impact of laser-assisted treatment modalities
  5. Announcement
  6. Pater Leander Fischer Prize 2014
  7. Magazine section
  8. Snapshots
  9. Original contributions
  10. Laser photobiomodulation influences the expression of genes related to the inflammatory process and muscle cell differentiation during the process of muscle healing
  11. Effects of low intensity laser irradiation during healing of infected skin wounds in the rat
  12. Analysis of enamel surface damage after selective laser ablation of composite from tooth surfaces
  13. Investigation of repulsion effects during lithotripsy
  14. Letter
  15. Replacing the scalpel with the laser reduces expenses in surgery – Taking the history of prostate therapy as an example
  16. Congress announcements
  17. 21. Annual Meeting of the Deutsche Gesellschaft für Lasermedizin (DGLM) e.V.
  18. Congresses 2014 / 2015
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