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Thermo-responsive poly(di(ethylene glycol) methyl ether methacrylate) brushes as substrate-independent release coatings for cell culture and selective cell separation and purification

  • Anna Schulte , Daniel Wesner , Mareike Müller and Holger Schönherr ORCID logo EMAIL logo
Published/Copyright: February 1, 2024
Pure and Applied Chemistry
From the journal Pure and Applied Chemistry Volume 96 Issue 2

Abstract

A systematic study on the surface-initiated polymerization of di(ethylene glycol) methyl ether methacrylate (DEGMA) by atom transfer radical polymerization (ATRP) from glass, silicon, titanium as well as tissue culture polystyrene (TCPS) is reported in an attempt to expand the known thermoresponsive poly(di(ethylene glycol) methyl ether methacrylate) (PDEGMA) cell release layers on gold to other substrates. The use of these substrate materials requires an altered immobilization chemistry to couple a bromide containing ATRP initiator to the surfaces. Using aminosilanes or polydopamine as coupling layers for the attachment of α-bromoisobutyryl bromide (BiBB) and the direct functionalization of surface hydroxyl groups with trichlorosilane-functionalized ATRP initiators all surfaces studied were shown to facilitate the growth of PDEGMA brushes using the same conditions that were reported previously for polymerization on gold. The brush layers obtained were characterized systematically using wetting, ellipsometry, X-ray photoelectron spectroscopy (XPS) as well as atomic force microscopy (AFM) analyses. Selective cell release and separation of PaTu 8988t and NIH 3T3 cells, which are known to exhibit different behavior after temperature drop-induced brush swelling, was observed for all substrates, albeit for different brush thicknesses, implying variations in initiator and also PDEGMA grafting density. The successful modification of biomedically relevant materials (Ti and TCPS) implies that the previously reported stem cell purification and selective cell release of various cell types, which is facilitated by PDEGMA brushes, can be realized and consequently scaled up in the future.

Keywords: cell purification; cell release layers; LCST; polymer brushes; POLY-CHAR 2023; thermal response
Corresponding author: Holger Schönherr, Department of Chemistry and Biology, Physical Chemistry I & Research Center of Micro and Nanochemistry and (Bio)Technology (Cμ), University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany, e-mail:

Article note: A collection of invited papers based on presentations at the International Polymer Characterization Forum POLY-CHAR 2023, held as an online meeting based in Auckland 22 January–26 January 2023.

Funding source: Universität Siegen

Funding source: European Regional Development Fund (EFRE)

Award Identifier / Grant number: project number EFRE0400308

Acknowledgment

The authors acknowledge Dr. Siyu Jiang for stimulating discussions as well as funding by the European Regional Development Fund (EFRE) under project number EFRE0400308 and the University of Siegen. Part of this work was performed at the Micro- and Nanoanalytics Facility (MNaF) at the University of Siegen.

  1. Research ethics: The DFG rules of good scientific practice were followed.

  2. Author contributions: HS and MM: Conceptualization, AS, DW and MM: methodology, AS and DW: formal analysis, AS, and DW: investigation, HS: resources, AS, DW: data curation, AS and DW: writing—original draft preparation, HS, MM: writing—review and editing, AS, DW and MM: visualization, HS and MM: supervision, HS, DW and MM: project administration, HS and MM: funding acquisition, HS. All authors have read and agreed to the published version of the manuscript.

  3. Competing interests: The authors declare no conflict of interest.

  4. Research funding: The authors acknowledge financial support by the European Regional Development Fund (EFRE) under project number EFRE0400308 and the University of Siegen.

  5. Data availability: The data presented in this study are available in this article (and supplementary material) or on request from the corresponding authors.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/pac-2023-1007).

Received: 2023-10-08
Accepted: 2024-01-11
Published Online: 2024-02-01
Published in Print: 2024-02-26

© 2024 IUPAC & De Gruyter

Articles in the same Issue

  1. Frontmatter
  2. In this issue
  3. Preface
  4. Preface for joint special issue of POLY-CHAR 2023 in Auckland, New Zealand and in memory of Professor Melissa Chan Chin Han
  5. Conference papers
  6. Simple preparation of silicone rubber Pickering emulsions using silica nanoparticles for water-borne thermal protection coating
  7. Thermo-responsive poly(di(ethylene glycol) methyl ether methacrylate) brushes as substrate-independent release coatings for cell culture and selective cell separation and purification
  8. The preparation of permanent antistatic additive based on poly(ether-b-amide) copolymers and its modification effect on polyamide 6
  9. Special topic papers
  10. Indentation creep in polymers and polymer nanocomposites
  11. What is science?
  12. Analysis of individual nanoscale block copolymer vesicles by atomic force microscopy combined with time-resolved fluorescence microscopy
  13. Green chemistry route to chitosan hydrogels and investigation of the materials as efficient dye adsorbents
  14. Flexible polymer networks: rubber elasticity and segmental orientation
https://doi.org/10.1515/pac-2023-1007
Keywords for this article
cell purification; cell release layers; LCST; polymer brushes; POLY-CHAR 2023; thermal response

Articles in the same Issue

  1. Frontmatter
  2. In this issue
  3. Preface
  4. Preface for joint special issue of POLY-CHAR 2023 in Auckland, New Zealand and in memory of Professor Melissa Chan Chin Han
  5. Conference papers
  6. Simple preparation of silicone rubber Pickering emulsions using silica nanoparticles for water-borne thermal protection coating
  7. Thermo-responsive poly(di(ethylene glycol) methyl ether methacrylate) brushes as substrate-independent release coatings for cell culture and selective cell separation and purification
  8. The preparation of permanent antistatic additive based on poly(ether-b-amide) copolymers and its modification effect on polyamide 6
  9. Special topic papers
  10. Indentation creep in polymers and polymer nanocomposites
  11. What is science?
  12. Analysis of individual nanoscale block copolymer vesicles by atomic force microscopy combined with time-resolved fluorescence microscopy
  13. Green chemistry route to chitosan hydrogels and investigation of the materials as efficient dye adsorbents
  14. Flexible polymer networks: rubber elasticity and segmental orientation
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