Home Preparation, characterization, and antimicrobial activity of novel chitosan blended almond gum–nanosilica bionanocomposite film for food packaging applications
Article
Licensed
Unlicensed Requires Authentication

Preparation, characterization, and antimicrobial activity of novel chitosan blended almond gum–nanosilica bionanocomposite film for food packaging applications

  • Ruby Thomas

    Dr. Ruby Thomas is currently an Assistant Professor in the Department of Chemistry, Loyola College Nungambakkam Chennai, Tamilnadu, India. She earned her PhD in Chemistry from Anna University Chennai, India. Her research interest includes surface science, nanotechnology, corrosion, coatings, and electrochemistry.

    EMAIL logo
    , Vinaya Thattil Vincent

    Ms.

    Vinaya Thattil Vincent was born in the year 2001. She has completed her postgraduation in Food Chemistry & Food Processing in the Department of Chemistry, Loyola College Nungambakkam Chennai.

    , Umapathy Manickam Janarthanam

    Dr.

    Umapathy Manickam Janarthanam is currently an Assistant Professor (Senior grade) in the Department of Chemistry, Anna University Chennai, India. His research interest includes polymer chemistry, material science, nanocomposites, corrosion, and electrochemistry.

    , Lakshmanan Rajagopal

    Mr.

    Lakshmanan Rajagopal has completed his graduation in Chemistry in the Department of Chemistry, Loyola College Nungambakkam Chennai. He was born in the year 2003.

    and Skandha Jay

    Mr.

    Skandha Jay is a graduate born in the year 2003. He has completed his B.Sc. Chemistry in the Department of Chemistry, Loyola College Nungambakkam Chennai.

Published/Copyright: September 27, 2023
Become an author with De Gruyter Brill

Abstract

Almond gum and varied concentrations of nanosilica (0.2, 0.4, 0.6, 0.8, and 1.0 wt%) were introduced into the chitosan polymer matrix by solution cast method to enrich the characteristics of the bionanocomposite film. The surface topography, thermal stability, crystalline nature, and functional moieties of the synthesized bionanocomposite films were characterized by SEM, TGA, XRD, and FT-IR. The UV–Vis spectrophotometer showed a maximum absorption wavelength for the film containing the highest concentration of nanosilica. Change in properties such as increased tensile strength, elongation and reduced water solubility, and swelling properties were observed for the bionanocomposite film containing 1.0 wt% nanosilica. In addition, the films exhibited excellent inhibition effect against Escherichia coli bacteria and Candida albicans fungus, which were proven by well diffusion assay method. The carrot slices packed in the bionanocomposite film containing the highest amount of nanosilica retained their freshness for a longer period of time, suggesting the film to be an effective and excellent food packaging material.


Corresponding author: Ruby Thomas, Department of Chemistry, Loyola College, Chennai, India, E-mail:

About the authors

Ruby Thomas

Dr. Ruby Thomas is currently an Assistant Professor in the Department of Chemistry, Loyola College Nungambakkam Chennai, Tamilnadu, India. She earned her PhD in Chemistry from Anna University Chennai, India. Her research interest includes surface science, nanotechnology, corrosion, coatings, and electrochemistry.

Vinaya Thattil Vincent

Ms.

Vinaya Thattil Vincent was born in the year 2001. She has completed her postgraduation in Food Chemistry & Food Processing in the Department of Chemistry, Loyola College Nungambakkam Chennai.

Umapathy Manickam Janarthanam

Dr.

Umapathy Manickam Janarthanam is currently an Assistant Professor (Senior grade) in the Department of Chemistry, Anna University Chennai, India. His research interest includes polymer chemistry, material science, nanocomposites, corrosion, and electrochemistry.

Lakshmanan Rajagopal

Mr.

Lakshmanan Rajagopal has completed his graduation in Chemistry in the Department of Chemistry, Loyola College Nungambakkam Chennai. He was born in the year 2003.

Skandha Jay

Mr.

Skandha Jay is a graduate born in the year 2003. He has completed his B.Sc. Chemistry in the Department of Chemistry, Loyola College Nungambakkam Chennai.

Acknowledgment

The authors thank the Management of Loyola College, Nungambakkam, Chennai for providing laboratory and chemical facilities for this research work.

  1. Research ethics: Not applicable.

  2. Author contributions: Dr. Ruby Thomas: methodology, material preparation, data analysis and review of manuscript. Ms. Vinaya Thattil Vincent: formal analysis, investigation, manuscript writing. Dr. Umapathy Manickam Janarthanam: methodology and conceptualization. Mr. Lakshmanan Rajagopal: data collection and experimental work. Mr. Skandha Jay: investigation, first draft of the manuscript.

  3. Competing interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

  4. Research funding: The author(s) received no financial support for the research, authorship, and/or publication of this article.

  5. Data availability: Data available on request from the authors.

References

[1] R. A. Ilyas, H. A. Aisyah, A. H. Nordin, et al.., “Natural-fiber-reinforced chitosan, chitosan blends and their nanocomposites for various advanced applications,” Polymers, vol. 14, no. 5, p. 874, 2022, https://doi.org/10.3390/polym14050874.Search in Google Scholar PubMed PubMed Central

[2] A. Kausar, “Progress in green nanocomposites for high-performance applications,” Mater. Res. Innovations, vol. 25, no. 1, pp. 53–65, 2021, https://doi.org/10.1080/14328917.2020.1728489.Search in Google Scholar

[3] V. Gupta, D. Biswas, and S. Roy, “A comprehensive review of biodegradable polymer-based films and coatings and their food packaging applications,” Materials, vol. 15, no. 17, pp. 58–99, 2022, https://doi.org/10.3390/ma15175899.Search in Google Scholar PubMed PubMed Central

[4] E. Díaz-Montes and R. Castro-Muñoz, “Edible films and coatings as food-quality preservers: an overview,” Foods, vol. 10, no. 2, p. 249, 2021, https://doi.org/10.3390/foods10020249.Search in Google Scholar PubMed PubMed Central

[5] R. Subramani, “Effects of almond gum/chitosan edible film and coating in postharvest fruits & vegetables to improving the shelf-life: food preservation,” SPAST Abstr., vol. 1, no. 01, 2021. Available at: https://spast.org/techrep/article/view/1860.Search in Google Scholar

[6] Z. Tahsiri, H. Mirzaei, S. M. H. Hosseini, and M. Khalesi, “Gum Arabic improves the mechanical properties of wild almond protein film,” Carbohydr. Polym., vol. 222, p. 114994, 2019. Available at: https://doi.org/10.1016/j.carbpol.2019.114994.Search in Google Scholar PubMed

[7] S. N. Suresh, C. Puspharaj, and R. Subramani, “Development of almond gum/alginate composites to enhance the shelf-life of post-harvest Solanum lycopersicum L,” Food Hydrocolloids Health, vol. 2, p. 100087, 2022, https://doi.org/10.1016/j.fhfh.2022.100087.Search in Google Scholar

[8] A. Saha, S. Tyagi, R. K. Gupta, and Y. K. Tyagi, “Natural gums of plant origin as edible coatings for food industry applications,” Crit Rev Biotechnol, vol. 37, no. 8, pp. 959–973, 2017, https://doi.org/10.1080/07388551.2017.1286449.Search in Google Scholar PubMed

[9] A. Taheri and S. M. Jafari, “Gum-based nanocarriers for the protection and delivery of food bioactive compounds,” Adv. Colloid Interface Sci., vol. 269, pp. 277–295, 2019, https://doi.org/10.1016/j.cis.2019.04.009.Search in Google Scholar PubMed

[10] R. K. Dhaka, N. Kumar, Pratibha, and A. Upadhyay, “Optimization, characterization, and influence of microfluidization on almond gum-based composite edible film,” Starch – Stärke, vol. 73, p. 2000101, 2021, https://doi.org/10.1002/star.202000101.Search in Google Scholar

[11] E. Alabaraoye, M. Achilonu, and R. Hester, “Biopolymer (Chitin) from various marine seashell wastes: isolation and characterization,” J. Polym. Environ., vol. 26, pp. 2207–2218, 2018, https://doi.org/10.1007/s10924-017-1118-y.Search in Google Scholar

[12] E. Atangana, T. T. Chiweshe, and H. Roberts, “Modification of novel chitosan-starch cross-linked derivatives polymers: synthesis and characterization,” J. Polym. Environ., vol. 27, pp. 979–995, 2019, https://doi.org/10.1007/s10924-019-01407-0.Search in Google Scholar

[13] C. N. Hernández-Téllez, M. Plascencia-Jatomea, and M. O. Cortez-Rocha, “Chitosan-based bionanocomposites: development and perspectives in food and agricultural applications,” Chitosan Preserv. Agricult. Commod., pp. 315–338, 2016, https://doi.org/10.1016/B978-0-12-802735-6.00012-4.Search in Google Scholar

[14] S. H. Othman, N. F. L. Othman, R. A. Shapi’i, S. H. Ariffin, K. F. M. Yunos, “Corn starch/chitosan nanoparticles/ThymolBio-nanocomposite films for potential food packaging applications,” Polymers, vol. 13, no. 3, pp. 390, 2021, https://doi.org/10.3390/polym13030390.Search in Google Scholar PubMed PubMed Central

[15] J. Sun, H. Jiang, H. Wu, C. Tong, J. Pang, and C. Wu, “Multifunctional bionanocomposite films based on konjac glucomannan/chitosan with nano-ZnO and mulberry anthocyanin extract for active food packaging,” Food Hydrocolloids, vol. 107, p. 105942, 2020, https://doi.org/10.1016/j.foodhyd.2020.105942.Search in Google Scholar

[16] M. Azmana, S. Mahmood, A. R. Hilles, A. Rahman, M. A. B. Arifin, and S. Ahmed, “A review on chitosan and chitosan-based bionanocomposites: promising material for combatting global issues and its applications,” Int. J. Biol. Macromol., vol. 185, pp. 832–848, 2021, https://doi.org/10.1016/j.ijbiomac.2021.07.023.Search in Google Scholar PubMed

[17] S. Amjadi, S. Emaminia, M. Nazari, S. HeyatDavudian, L. Roufegarinejad, and H. Hamishehkar, “Application of reinforced ZnO nanoparticle-incorporated gelatin bionanocomposite film with chitosan nanofiber for packaging of chicken fillet and cheese as food models,” Food Bioprocess Technol., vol. 12, pp. 1205–1219, 2019, https://doi.org/10.1007/s11947-019-02286-y.Search in Google Scholar

[18] S. Mallakpour, F. Sirous, and C. M. Hussain, “A journey to the world of fascinating ZnO nanocomposites made of chitosan, starch, cellulose, and other biopolymers: progress in recent achievements in eco-friendly food packaging, biomedical, and water remediation technologies,” Int. J. Biol. Macromol., vol. 170, pp. 701–716, 2021, https://doi.org/10.1016/j.ijbiomac.2020.12.163.Search in Google Scholar PubMed

[19] R. Kumar, B. Rai, and G. A. Kumar, “A simple approach for the synthesis of cellulose nanofiber reinforced chitosan/PVP bio nanocomposite film for packaging,” J. Polym. Environ., vol. 27, pp. 2963–2973, 2019, https://doi.org/10.1088/2053-1591/ab3511.Search in Google Scholar

[20] B. Fu, Q. Liu, M. Liu, et al.., “Carbon dots enhanced gelatin/chitosan bio-nanocomposite packaging film for perishable foods,” Chin. Chem. Lett., vol. 33, no. 10, pp. 4577–4582, 2022, https://doi.org/10.1016/j.cclet.2022.03.048.Search in Google Scholar

[21] K. El Bourakadi, N. Merghoub, M. Fardioui, et al.., “Chitosan/polyvinyl alcohol/thiabendazoluim-montmorillonite bio-nanocomposite films: mechanical, morphological and antimicrobial properties,” Composites, Part B, vol. 172, pp. 103–110, 2019, https://doi.org/10.1016/j.compositesb.2019.05.042.Search in Google Scholar

[22] V. G. L. Souza, C. Rodrigues, S. Valente, et al.., “Eco-friendly ZnO/chitosan bionanocomposites films for packaging of fresh poultry meat,” Coatings, vol. 10, no. 2, pp.110, 2020, https://doi.org/10.3390/coatings10020110.Search in Google Scholar

[23] T. T. Thendral and N. Rajeswari, “Effect of nanosilica and neem tree oil on antimicrobial, thermal, mechanical and electrical insulate of biodegradable composite film,” Mater. Res. Express, vol. 6, p. 095410, 2019. Available at: https://doi.org/10.1088/2053-1591/ab30a1.Search in Google Scholar

[24] J. Cheng, B. Tian, J. Wang, Z. Wang, and Y. Liu, “Development of multifunctional films based on chitosan, nano silica and hops extracts,” Eur. Polym. J., vol. 161, pp. 110–816, 2021, https://doi.org/10.1016/j.eurpolymj.2021.110816.Search in Google Scholar

[25] Z. Wu, Y. Li, J. Tang, et al.., “Ultrasound-assisted preparation of chitosan/nano-silica aerogel/tea polyphenol biodegradable films: physical and functional properties,” Ultrason. Sonochem., vol. 87, 2022, Art. no. 106052, https://doi.org/10.1016/j.ultsonch.2022.106052.Search in Google Scholar PubMed PubMed Central

[26] R. Zhang, X. Wang, J. Wang, M. Cheng, “Synthesis and characterization of konjac glucomannan/carrageenan/nano-silica films for the preservation of postharvest white mushrooms,” Polymers, vol. 1, no. 1, pp. 6, 2019, https://doi.org/10.3390/polym11010006.Search in Google Scholar PubMed PubMed Central

[27] M. Alghdeir, K. Mayya, and M. Dib, “Characterization of nanosilica/low-density polyethylene nanocomposite materials,” J. Nanomater., vol. 2019, p. 8, Art. no. 4184351, 2019. https://doi.org/10.1155/2019/4184351.Search in Google Scholar

[28] B. R. B. Lara, P. S. de Andrade, M. G. Junior, M. V. Dias, and L. A. P. Alcântara, “Novel whey protein isolate/polyvinyl biocomposite for packaging: improvement of mechanical and water barrier properties by incorporation of nano-silica,” J. Polym. Environ., vol. 29, pp. 2397–2408, 2021, https://doi.org/10.1007/s10924-020-02033-x.Search in Google Scholar

[29] Y. Cui, M. Cheng, M. Han, R. Zhang, and X. Wang, “Characterization and release kinetics study of potato starch nanocomposite films containing mesoporous nano-silica incorporated with Thyme essential oil,” Int. J. Biol. Macromol., vol. 184, pp. 566–573, 2021, https://doi.org/10.3390/polym14061214.Search in Google Scholar PubMed PubMed Central

[30] G. Umamaheswari, S. Sanuja, V. A. John, S. V. Kanth, and M. J. Umapathy, “Preparation, characterization and anti-bacterial activity of zinc oxide-gelatin nanocomposite film for food packaging applications,” Polym. Polym. Compos., vol. 23, no. 3, pp. 199–204, 2015, https://doi.org/10.1177/096739111502300311.Search in Google Scholar

[31] S. Sanuja, A. Agalya, and M. J. Umapathy, “Studies on magnesium oxide reinforced chitosan bionanocomposite incorporated with clove oil for active food packaging application,” Int. J. Polym. Mater., vol. 63, no. 14, pp. 733–740, 2014, https://doi.org/10.1080/00914037.2013.879445.Search in Google Scholar

[32] V. Dhapte, S. Kadam, V. Pokharkar, P. K. Khanna, and V. Dhapte, “Versatile SiO2 nanoparticles @polymer composites with pragmatic properties,” Int. Sch. Res. Notices, vol. 2014, p. 8, Art. no. 170919, 2014, https://doi.org/10.1155/2014/170919.Search in Google Scholar

[33] W. Lan, S. Li, S. Shama, et al.., “Investigation of ultrasonic treatment on physicochemical, structural and morphological properties of sodium alginate/AgNPs/apple polyphenol films and its preservation effect on strawberry,” Polymer, vol. 12, no. 9, pp. 2096, 2020, https://doi.org/10.3390/polym12092096.Search in Google Scholar PubMed PubMed Central

[34] S. Sanuja, A. Agalya, and M. J. Umapathy, “Synthesis and characterization of zinc oxide-neem oil-chitosan bionanocomposite for food packaging application,” Int. J. Biol. Macromol., vol. 74, pp. 76–84, 2014, https://doi.org/10.1016/j.ijbiomac.2014.11.036.Search in Google Scholar PubMed

[35] S. Mallakpour and V. Behranvand, “Nanocomposites based on biosafe nano ZnO and different polymeric matrixes for antibacterial, optical, thermal and mechanical applications,” Eur. Polym. J., vol. 84, pp. 377–403, 2016, https://doi.org/10.1016/j.eurpolymj.2016.09.028.Search in Google Scholar

[36] S. Kumar-Krishnan, E. Prokhorov, M. Hernández-Iturriaga, et al.., “Chitosan/silver nanocomposites: synergistic antibacterial action of silver nanoparticles and silver ions,” Eur. Polym. J., vol. 67, pp. 242–251, 2015, https://doi.org/10.1016/j.eurpolymj.2015.03.066.Search in Google Scholar

[37] V. H. Campos-Requena, B. L. Rivas, M. A. Pérez, K. A. Garrido-Miranda, and E. D. Pereira, “Release of essential oil constituent from thermoplastic starch/layered silicate bionanocomposite film as a potential active packaging material,” Eur. Polym. J., vol. 109, pp. 64–71, 2018, https://doi.org/10.1016/j.eurpolymj.2018.08.055.Search in Google Scholar

[38] R. H. Tabatabaei, S. M. Jafari, H. Mirzaei, A. M. Nafchi, and D. Dehnad, “Preparation and characterization of nano-SiO2 reinforced gelatin-k-carrageenan biocomposites,” Int. J. Biol. Macromol., vol. 111, pp. 1091–1099, 2018, https://doi.org/10.1016/j.ijbiomac.2018.01.116.Search in Google Scholar PubMed

[39] K. T. Nguyen, D. X. N. Mai, U. T. T. Doan, et al.., “The chitosan/ZnO bio-nanocomposites with selective antibacterial efficiency,” J. Mater. Res., vol. 36, pp. 508–517, 2021, https://doi.org/10.1557/s43578-020-00011-6.Search in Google Scholar

[40] M. A. López-Mata, S. Ruiz-Cruz, J. D. J. Ornelas-Paz, et al.., “Mechanical, barrier and antioxidant properties of chitosan films incorporating cinnamaldehyde,” J. Polym. Environ., vol. 26, pp. 452–461, 2017, https://doi.org/10.1007/s10924-017-0961-1.Search in Google Scholar

[41] S. Mohammadi, A. Babaei, and Z. A. Bafrani, “Polyethylene glycol-decorated GO nanosheets as a well-organized nanohybrid to enhance the performance of chitosan biopolymer,” J. Polym. Environ., vol. 30, pp. 5130–5147, 2022, https://doi.org/10.1007/s10924-022-02577-0.Search in Google Scholar

[42] A. George, P. A. Shah, and P. S. Shrivastav, “Guar gum: versatile natural polymer for drug delivery applications,” Eur. Polym. J., vol. 112, pp. 722–735, 2019, https://doi.org/10.1016/j.eurpolymj.2018.10.042.Search in Google Scholar

[43] P. Chen, F. Xie, F. Tang, and T. McNally, “Influence of plasticiser type and nanoclay on the properties of chitosan-based materials,” Eur. Polym. J., vol. 144, p. 110225, 2021. https://doi.org/10.1016/j.eurpolymj.2020.110225.Search in Google Scholar

[44] A. Gunoz, Y. Kepir, and M. Kara, “Effect of hydrothermal aging on the mechanical properties of nanocomposite pipes,” Mater. Test., vol. 63, no. 3, pp. 253–258, 2021, https://doi.org/10.1515/mt-2020-0037.Search in Google Scholar

[45] K. González, L. Iturriaga, A. González, A. Eceiza, and N. Gabilondo, “Improving mechanical and barrier properties of thermoplastic starch and polysaccharide nanocrystals nanocomposites,” Eur. Polym. J., vol. 29, pp. 2397–2408, 2021, https://doi.org/10.1016/j.eurpolymj.2019.109415.Search in Google Scholar

[46] H. S. Hedia, S. M. Aldousari, A. Khairy, E. Aljabarti, “Fatigue life behaviour of nanocomposite coated carbon steel,” Mater. Test., vol. 54, no. 4, pp. 249–256, 2012, https://doi.org/10.3139/120.110325.Search in Google Scholar

[47] S. Rani and R. Kumar, “A review on material and antimicrobial properties of soy protein isolate film,” J. Polym. Environ., vol. 27, no. 8, pp. 1613–1628, 2019, https://doi.org/10.1007/s10924-019-01456-5.Search in Google Scholar

[48] S. Ediyilyam, B. George, S. S. Shankar, et al.., “Chitosan/Gelatin/Silver nanoparticles composites films for biodegradable food packaging applications,” Polymers, vol. 13, no. 11, p. 1680, 2021, https://doi.org/10.3390/polym13111680.Search in Google Scholar PubMed PubMed Central

[49] R. Priyadarshi and Y. S. Negi, “Effect of varying filler concentration on zinc oxide nanoparticle embedded chitosan films as potential food packaging material,” J. Polym. Environ., vol. 25, no. 4, pp. 1087–1098, 2017, https://doi.org/10.1007/s10924-016-0890-4.Search in Google Scholar

[50] L. Al-naamani, S. Dobretsov, and J. Dutta, “Chitosan-zinc oxide nanoparticle composite coating for active food packaging applications,” Innov. Food Sci. Emerg. Technol., vol. 38, pp. 231–237, 2016, https://doi.org/10.1016/j.ifset.2016.10.010.Search in Google Scholar

[51] M. Shamshirsaz, A. Fereidoon, A. Albooyeh, and I. Danaee, “Corrosion resistance, thermal diffusivity and mechanical properties of Ni–SiO2 nanocomposite coatings on a 316 stainless steel for heat exchanger applications,” Mater. Test., vol. 64, no. 12, pp. 1733–1752, 2022, https://doi.org/10.1515/mt-2022-0024.Search in Google Scholar

[52] T. J. Gutiérrez, “Active and intelligent films made from starchy sources/blackberry pulp,” J. Polym. Environ., vol. 26, pp. 2374–2391, 2018, https://doi.org/10.1007/s10924-017-1134-y.Search in Google Scholar

[53] A. M. Abdel Ghaffar, H. E. Ali, S. M. Nasef, et al.., “Effect of gamma radiation on the properties of crosslinked chitosan nano-composite film,” J. Polym. Environ., vol. 26, pp. 3226–3236, 2018, https://doi.org/10.1007/s10924-018-1208-5.Search in Google Scholar

[54] A. A. Ahmad and N. M. Sarbon, “A comparative study: physical, mechanical and antibacterial properties of bio-composite gelatin films as influenced by chitosan and zinc oxide nanoparticles incorporation,” Food Biosci., vol. 43, p. 101250, 2021, https://doi.org/10.1016/j.fbio.2021.101250.Search in Google Scholar

Published Online: 2023-09-27
Published in Print: 2023-12-15

© 2023 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Effects of shear deformation on grain size and mechanical properties of the forged B4Cp/Al composite
  3. A novel method for measurements of surface topography in previously inaccessible areas
  4. Optimum design of a seat bracket using artificial neural networks and dandelion optimization algorithm
  5. Comparison between laser and TIG welding of electron beam melted Ti6Al4V parts
  6. Influence of heat input on temperature and stress field of X80 steel pipeline cirumferential weld using type-B sleeve repairing
  7. Experimental investigation of mechanical properties of PLA, ABS, and PETG 3-d printing materials using fused deposition modeling technique
  8. Preparation, characterization, and antimicrobial activity of novel chitosan blended almond gum–nanosilica bionanocomposite film for food packaging applications
  9. A novel hybrid Fick’s law algorithm-quasi oppositional–based learning algorithm for solving constrained mechanical design problems
  10. Tensile, bending, and impact properties of laminated carbon/aramid/glass hybrid fiber composites
  11. Effect of welding processes on ferrite content, microstructure and mechanical properties of super duplex stainless steel 2507 welds
  12. Wear and residual stress in high-feed milling of AISI H13 tool steel
  13. Optimum design of a composite drone component using slime mold algorithm
  14. Lateral compression behavior of expanded polypropylene foam–filled carbon and glass fiber composite tubes
  15. Effect of red mud on mechanical and thermal properties of agave sisalana/glass fiber–reinforced hybrid composites
  16. Mechanical analysis of composite plates adhesively joined with different single-lap techniques under bending loading
Downloaded on 15.9.2025 from https://www.degruyterbrill.com/document/doi/10.1515/mt-2023-0092/html
Scroll to top button