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High mobility group box 1 in women with unexplained recurrent pregnancy loss

  • Carlo Ticconi ORCID logo , Stefania Mardente ORCID logo , Emanuela Mari , Federica Barreca , Manuela Montanaro ORCID logo , Alessandro Mauriello ORCID logo , Giuseppe Rizzo ORCID logo EMAIL logo and Alessandra Zicari ORCID logo
Published/Copyright: May 30, 2023
Journal of Perinatal Medicine
From the journal Journal of Perinatal Medicine Volume 51 Issue 9

Abstract

Objectives

To investigate whether high mobility group box 1 (HMGB1) is involved in unexplained recurrent pregnancy loss (uRPL).

Methods

Plasma levels of HMGB1 were measured by ELISA in non-pregnant women with (n=44) and without (n=53 controls) uRPL. Their platelets and plasma-derived microvesicles (MVs) were also assayed for HMGB1. Endometrial biopsies were taken in selected uRPL (n=5) and control women (n=5) and the tissue expression of HMGB1 was determined by western blot and immunohistochemistry (IHC).

Results

plasma levels of HMGB1 were significantly higher in women with uRPL than in control women. HMGB1 content in platelets and MVs obtained from women with uRPL was significantly higher than that obtained from control women. HMGB1 expression in endometrium was higher in tissues obtained from women with uRPL than in tissues obtained from control women. IHC analysis revealed that HMGB1 is expressed in endometrium with different patterns between uRPL and control women.

Conclusions

HMGB1 could be involved in uRPL.

Keywords: endometrium; high mobility group box 1 (HMGB1); microvesicles; recurrent pregnancy loss
Corresponding author: Prof Giuseppe Rizzo, Department of Obstetrics and Gynecology, University of Rome “Tor Vergata”, Fondazione Policlinico Tor Vergata, Viale Oxford 81, 00133, Rome, Italy, E-mail:
C. Ticconi and S. Mardente contributed equally to this work.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: The study was approved by the Institutional Review Board (IRB) of Policlinico Tor Vergata University Hospital (protocol number: 242/21).

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Received: 2023-03-11
Accepted: 2023-05-13
Published Online: 2023-05-30
Published in Print: 2023-11-27

© 2023 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Obituary
  3. A tribute to Professor Moshe Mazor, M.D.
  4. Mini Review
  5. Systematic review of the long-term effects of postnatal corticosteroids
  6. Opinion Paper
  7. The proposal of the novel fetal shoulder dystocia graduation: a clinical-based opinion
  8. Corner of Academy
  9. Prenatal diagnosis of bilobate placenta: incidence, risk factors and impact on pregnancy outcomes
  10. Original Articles – Obstetrics
  11. High mobility group box 1 in women with unexplained recurrent pregnancy loss
  12. Velamentous cord insertion in monochorionic twin pregnancies: a step forward in screening for twin to twin transfusion syndrome and birthweight discordance?
  13. Advanced maternal age (AMA) and 75 g oGTT glucose levels are pedictors for insulin therapy in women with gestational diabetes (GDM)
  14. Perinatal, obstetric and parental risk factors for asthma in the offspring throughout childhood: a longitudinal cohort study
  15. Increased risk of severe COVID-19 in pregnancy in a multicenter propensity score-matched study
  16. Comparative clinical and placental pathologic characteristics in pregnancies with and without SARS-CoV-2 infection
  17. An analysis of factors affecting survival in prenatally diagnosed omphalocele
  18. The impact of abnormal maternal body mass index during pregnancy on perinatal outcomes: a registry-based study from Qatar
  19. Original Articles – Fetus
  20. Embryonic and fetal tiny pericardial fluid collections at less than 12 weeks of gestation
  21. Modeling fetal cortical development by quantile regression for gestational age and head circumference: a prospective cross sectional study
  22. The effect of 50 GR oral glucose tolerance test on fetal celiac artery and superior mesenteric artery Doppler parameters in healthy pregnancies
  23. Original Articles – Neonates
  24. Carboxyhaemoglobin levels in infants with hypoxic ischaemic encephalopathy
  25. Exploring professionals’ views regarding prenatal counselling in congenital diaphragmatic hernia
  26. Letter to the Editor
  27. Cutting of the strangulated double nuchal umbilical cord in a release of the severe shoulder dystocia: forensically justified or controversial procedure
  28. Retraction
  29. Retraction of: Pre-operative tranexemic acid vs. etamsylate in reducing blood loss during elective cesarean section: randomized controlled trial
  30. Retraction of: Lidocaine vs. tramadol vs. placebo wound infiltration for post-cesarean section pain relief: a randomized controlled trial
https://doi.org/10.1515/jpm-2023-0109
Keywords for this article
endometrium; high mobility group box 1 (HMGB1); microvesicles; recurrent pregnancy loss

Articles in the same Issue

  1. Frontmatter
  2. Obituary
  3. A tribute to Professor Moshe Mazor, M.D.
  4. Mini Review
  5. Systematic review of the long-term effects of postnatal corticosteroids
  6. Opinion Paper
  7. The proposal of the novel fetal shoulder dystocia graduation: a clinical-based opinion
  8. Corner of Academy
  9. Prenatal diagnosis of bilobate placenta: incidence, risk factors and impact on pregnancy outcomes
  10. Original Articles – Obstetrics
  11. High mobility group box 1 in women with unexplained recurrent pregnancy loss
  12. Velamentous cord insertion in monochorionic twin pregnancies: a step forward in screening for twin to twin transfusion syndrome and birthweight discordance?
  13. Advanced maternal age (AMA) and 75 g oGTT glucose levels are pedictors for insulin therapy in women with gestational diabetes (GDM)
  14. Perinatal, obstetric and parental risk factors for asthma in the offspring throughout childhood: a longitudinal cohort study
  15. Increased risk of severe COVID-19 in pregnancy in a multicenter propensity score-matched study
  16. Comparative clinical and placental pathologic characteristics in pregnancies with and without SARS-CoV-2 infection
  17. An analysis of factors affecting survival in prenatally diagnosed omphalocele
  18. The impact of abnormal maternal body mass index during pregnancy on perinatal outcomes: a registry-based study from Qatar
  19. Original Articles – Fetus
  20. Embryonic and fetal tiny pericardial fluid collections at less than 12 weeks of gestation
  21. Modeling fetal cortical development by quantile regression for gestational age and head circumference: a prospective cross sectional study
  22. The effect of 50 GR oral glucose tolerance test on fetal celiac artery and superior mesenteric artery Doppler parameters in healthy pregnancies
  23. Original Articles – Neonates
  24. Carboxyhaemoglobin levels in infants with hypoxic ischaemic encephalopathy
  25. Exploring professionals’ views regarding prenatal counselling in congenital diaphragmatic hernia
  26. Letter to the Editor
  27. Cutting of the strangulated double nuchal umbilical cord in a release of the severe shoulder dystocia: forensically justified or controversial procedure
  28. Retraction
  29. Retraction of: Pre-operative tranexemic acid vs. etamsylate in reducing blood loss during elective cesarean section: randomized controlled trial
  30. Retraction of: Lidocaine vs. tramadol vs. placebo wound infiltration for post-cesarean section pain relief: a randomized controlled trial
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