Comparison of serum 25-hydroxyvitamin D levels between radioimmunoassay and liquid chromatography-tandem mass spectrometry in infants and postpartum women
-
Kaori Hara
,
Kazushige Ikeda
Abstract
Background
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the gold standard for the measurement of serum 25-hydroxyvitamin D (25(OH)D) levels instead of the conventional method, radioimmunoassay (RIA). However, there was no study that compared RIA and LC-MS/MS for measuring serum 25(OH)D levels in infants and their mothers. The aim of this study was to assess the agreement of RIA and LC-MS/MS for measuring the serum levels in infants and postpartum women.
Methods
This study enrolled 70 preterm infants, 113 term infants (134 samples), and 120 postpartum women. Serum concentration of 25(OH)D was measured by RIA and LC-MS/MS. We evaluated the correlation between RIA and LC-MS/MS. Also, we evaluated the bias between RIA and LC-MS/MS using Bland-Altman analysis.
Results
Sixty percent of preterm infants had serum 25(OH)D levels below the lower limit of quantification (LOQ) (4 ng/mL) and 90% of them were classified as vitamin D deficient. The serum 25(OH)D levels measured by RIA were significantly correlated with those measured by LC-MS/MS in all groups. According to the Bland-Altman plot, the serum 25(OH)D levels of infants measured by RIA had constant positive bias (mean±standard deviation [SD] [95% confidence interval, CI], preterm: +4.8± 2.4 ng/mL [4.2–5.4], term: +5.8±4.0 [5.1–6.5]) and proportional bias (preterm: r=0.44, p<0.01, term: r=0.50, p<0.01) compared with LC-MS/MS. The serum 25(OH)D levels of postpartum women measured by RIA had constant positive bias compared with LC-MS/MS, but no proportional bias was found.
Conclusions
RIA demonstrated falsely high 25(OH)D levels when used for infants and postpartum women.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None to declared.
Employment of readership: None declared.
Honorarium: None declared.
Competing interests: None declared.
References
1. Feldman D. Vitamin D, 3rd ed. London: Academic Press, 2011:5–6.Search in Google Scholar
2. Munns CF, Shaw N, Kiely M, Specker BL, Thacher TD, et al. Global consensus recommendations on prevention and management of nutritional rickets. J Clin Endocrinol Metab 2016;101:394–415.10.1210/jc.2015-2175Search in Google Scholar PubMed PubMed Central
3. Siafarikas A, Deishi A, Jahreis G, Pieplow A, Vogel H, et al. Cross-sectional analysis of universal vitamin D supplementation in former East Germany during the first year of life. J Pediatr Endocrinol Metab 2017;30:395–404.10.1515/jpem-2016-0310Search in Google Scholar PubMed
4. Anguana SK, Angurana RS, Mahajan G, Kumar N, Mahajan V. Prevalence of vitamin D deficiency in apparently healthy children in north India. J Pediatr Endocrinol Metab 2014;27:1151–6.Search in Google Scholar
5. Shah I, James R, Barker J, Petroczi A, Naughton DP. Misleading measures in vitamin D analysis: a novel LC-MS/MS assay to account for epimer and isobars. Nutrition J 2011;10:46.10.1186/1475-2891-10-46Search in Google Scholar PubMed PubMed Central
6. Hollis BW. The determination of circulating 25-hydroxyvitamin D: no easy task. J Clin Endocr Metab 2004;89:3149–51.10.1210/jc.2004-0682Search in Google Scholar PubMed
7. Schottker B, Jansen EH, Haug U, Schomburg L, Kohrle J, et al. Standardization of misleading immunoassay based 25-hydroxyvitamin D levels with liquid chromatography tandem-mass spectrometry in a large cohort study. PLoS One 2012;7:e48774.10.1371/journal.pone.0048774Search in Google Scholar PubMed PubMed Central
8. van den Ouweland JM, Beijers AM, van Daal H, Elisen MG, Steen G, et al. Evaluation of 3-epi-25-hydroxyvitamin D3 cross-reactivity in the Roche Elecsys vitamin D total protein binding assay. Clin Chem Lab Med 2014;52:373–80.10.1515/cclm-2013-0702Search in Google Scholar PubMed
9. Volmer DA, Mendes LR, Stokes CS. Analysis of vitamin D metabolic markers by mass spectrometry: current techniques, limitations of the “gold standard” method, and anticipated future directions. Mass Spectrom Rev 2015;34:2–23.10.1002/mas.21408Search in Google Scholar PubMed
10. Farrell CL, Martin S, McWhinney B, Straub I, Williams P, et al. State-of-the art vitamin D assays: a comparison of automated immunoassays with liquid chromatography-tandem mass spectrometry methods. Clin Chem 2012;58:531–42.10.1373/clinchem.2011.172155Search in Google Scholar PubMed
11. van den Ouweland JM, Beijers AM, Demacker P, van Daal H. Measurement of 25-OH-vitamin D in human serum using liquid chromatography tandem-mass spectrometry with comparison to radioimmunoassay and automated immunoassay. J Choromatogr B 2010;878:1163–8.10.1016/j.jchromb.2010.03.035Search in Google Scholar PubMed
12. Yun C, Chen J, Yang C, Li Y, Piao J, et al. Comparison of two 25-hydroxyvitamin D immunoasssays to liquid chromatography-tandem mass spectrometry in assessing samples from the Chinese population. Clin Chim Acta 2015;448:22–6.10.1016/j.cca.2015.06.007Search in Google Scholar PubMed
13. 25-Hydroxyvitamin D 125I RIA kit instruction manual. Diasorin, REF 68100E.Search in Google Scholar
14. Molloy B, Calton L, Cooper D. An offline automated solid-phase extraction method for measurement of serum 25-hydroxyvitamin D for clinical research. 2014. Waters, Application Note 72003139EN.Search in Google Scholar
15. Holick MF. Vitamin D deficiency. New Engl J Med 2007;357:266–81.10.1056/NEJMra070553Search in Google Scholar PubMed
16. Aghajafari F, Field CJ, Rabi D, Kaplan BJ, Maggiore JA, et al. Plasma 3-epi-25-hydroxycholecalciferol can alter the assessment of vitamin D status using the current reference ranges for pregnant women and their newborns. J Nutr 2016;146:70–5.10.3945/jn.115.220095Search in Google Scholar PubMed
17. Bailey D, Perumal N, Yazdanpanah M, Al Mahmud A, Baqui AH, et al. Maternal-fetal-infant dynamics of the C3-epimer of 25-hydroxyvitamin D. Clin Biochem 2014;47:816–22.10.1016/j.clinbiochem.2014.01.015Search in Google Scholar PubMed
18. Hara K, Ikeda L, Koyama Y, Wada Y, Hasegawa T. Serum 25-hydroxyvitamin D3 levels of one-month old term infants in Tokyo using liquid chromatography tandem mass spectrometry. Acta Paediatr 2018;107:532–3.10.1111/apa.14155Search in Google Scholar PubMed
19. Strathmann FG, Sadilkova K, Laha TJ, LeSourd SE, Bornhorst JB, et al. 3-epi-25 hydroxyvitamin D concentrations are not correlated with age in a cohort of infants and adults. Clin Chim Acta 2012;413:203–6.10.1016/j.cca.2011.09.028Search in Google Scholar PubMed PubMed Central
20. Granado-Lorencio F, Garcia-Heras LM, Blanco-Navarro I, Perez-Sacristan B. Assessment of 3-epi-25-OH-D3 in preterm and full term infant samples and its relationship to demographic, anthropometric and biochemical determinants. Clin Biochem 2014;47:853–6.10.1016/j.clinbiochem.2014.02.001Search in Google Scholar PubMed
21. Bartoszewicz Z, Kondracka A, Jazwiec R, Popow M, Dadlez M, et al. Can we accurately measure the concentration of clinically relevant vitamin D metabolites in the circulation? The problems and their consequences. Endokrynol Pol 2013;64:238–45.Search in Google Scholar
22. Higashi T, Goto A, Murohashi M, Ogawa A, Komatsu K, et al. Development and validation of a method for determination of plasma 25-hydroxyvitamin D3 3-sulfate using liquid chromatograph/tandem mass spectrometry. J Chromatgr B Analyt Technol Biomed Life Sci 2014;969:230–4.10.1016/j.jchromb.2014.08.027Search in Google Scholar PubMed
23. Higashi T, Yokota M, Goto A, Komatsu K, Sugiura T, et al. A method for simultaneous determination of 25-hydroxyvitamin D3 and its 3-sulfate in newborn plasma by LC/ESI-MS/MS after derivatization with a proton-affinitive cookson-type reagent. Mass Spectrom 2016;5:S0051.10.5702/massspectrometry.S0051Search in Google Scholar PubMed PubMed Central
24. Nakamura K, Nashimoto M, Yamamoto M. Summer/winter differences in the serum 25-hydroxyvitamin D3 and parathyroid hormone levels of Japanese women. Int J Biometeorol 2000;44:186–9.10.1007/s004840000067Search in Google Scholar PubMed
25. Ono Y, Suzuki A, Kotake M, Zhang X, Nishiwaki-Yasuda K, et al. Seasonal changes of serum 25-hydroxyvitamin D and intact parathyroid hormone levels in a normal Japanese population. J Bone Miner Metab 2005;23:147–51.10.1007/s00774-004-0553-8Search in Google Scholar PubMed
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Articles in the same Issue
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- Achieving target levels for vascular risk parameters in Polish school-age children with type 1 diabetes – a single center study
- Adipocyte aquaporin 7 (AQP7) expression in lean children and children with obesity. Possible involvement in molecular mechanisms of childhood obesity
- Use of complementary and alternative medicine in patients with inherited metabolic disease
- Are breast milk adipokines affected by maternal dietary factors?
- Comparison of serum 25-hydroxyvitamin D levels between radioimmunoassay and liquid chromatography-tandem mass spectrometry in infants and postpartum women
- Reference intervals for neonatal thyroid function tests in the first 7 days of life
- Treatment outcomes in pediatric differentiated thyroid carcinoma
- Seasonality of month of birth in children and adolescents with autoimmune thyroiditis: a continuing conundrum
- Follow-up in children with non-obese and non-autoimmune subclinical hypothyroidism
- Glucose intolerance in children with cystic fibrosis: a developing country’s perspective
- Letter to the Editor
- A patient with classical galactosemia is graduated with a university degree
- Case Reports
- Prepubertal gynaecomastia in a boy with Peutz-Jeghers syndrome: managing the aromatase overexpression
- Hypogammaglobulinemia and imaging features in a patient with infantile free sialic acid storage disease (ISSD) and a novel mutation in the SLC17A5 gene
- Xanthogranuloma of the sellar region accompanied by growth hormone deficiency: case report and literature review
- A case of Graves’ disease associated with membranoproliferative glomerulonephritis and leukocytoclastic vasculitis
Articles in the same Issue
- Frontmatter
- Original Articles
- The associations between mental health, health-related quality of life and insulin pump therapy among children and adolescents with type 1 diabetes
- Achieving target levels for vascular risk parameters in Polish school-age children with type 1 diabetes – a single center study
- Adipocyte aquaporin 7 (AQP7) expression in lean children and children with obesity. Possible involvement in molecular mechanisms of childhood obesity
- Use of complementary and alternative medicine in patients with inherited metabolic disease
- Are breast milk adipokines affected by maternal dietary factors?
- Comparison of serum 25-hydroxyvitamin D levels between radioimmunoassay and liquid chromatography-tandem mass spectrometry in infants and postpartum women
- Reference intervals for neonatal thyroid function tests in the first 7 days of life
- Treatment outcomes in pediatric differentiated thyroid carcinoma
- Seasonality of month of birth in children and adolescents with autoimmune thyroiditis: a continuing conundrum
- Follow-up in children with non-obese and non-autoimmune subclinical hypothyroidism
- Glucose intolerance in children with cystic fibrosis: a developing country’s perspective
- Letter to the Editor
- A patient with classical galactosemia is graduated with a university degree
- Case Reports
- Prepubertal gynaecomastia in a boy with Peutz-Jeghers syndrome: managing the aromatase overexpression
- Hypogammaglobulinemia and imaging features in a patient with infantile free sialic acid storage disease (ISSD) and a novel mutation in the SLC17A5 gene
- Xanthogranuloma of the sellar region accompanied by growth hormone deficiency: case report and literature review
- A case of Graves’ disease associated with membranoproliferative glomerulonephritis and leukocytoclastic vasculitis
