Microarray analysis of slipped capital femoral epiphysis growth plates

Jeffrey S. Johnson; Dennis S. Weiner; Robin Jacquet; Mark J. Adamczyk; Melanie A. Morscher; William J. Landis

doi:10.1515/jpem-2016-0023

Article

Microarray analysis of slipped capital femoral epiphysis growth plates

Jeffrey S. Johnson , Dennis S. Weiner , Robin Jacquet , Mark J. Adamczyk , Melanie A. Morscher and William J. Landis

Published/Copyright: July 8, 2016

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From the journal Journal of Pediatric Endocrinology and Metabolism Volume 29 Issue 8

Abstract

Background:

Microarray technology has been used to analyze gene expression in patients with and without slipped capital femoral epiphysis (SCFE).

Methods:

Proximal femoral physis core biopsies from two patients with SCFE were compared with two control specimens from age-matched patients without SCFE. Extracted RNA from frozen ground samples was subjected to microarray analysis with data tests for statistical significance between SCFE and control tissues.

Results:

Compared to controls, SCFE samples demonstrated significant up-regulation in gene expression pathways involving physiological defense and inflammatory responses and significant down-regulation in the regulation of cellular physiologic processes, cellular metabolic pathways, and skeletal development pathways including expression of aggrecan and type II collagen, genes affecting physeal structure and integrity.

Conclusions:

Up-regulation of inflammatory and immune response pathways in SCFE compared to controls relates to physeal mechanical displacement in SCFE. Globalized down-regulation of several other pathways suggests growth plate weakening. These novel microarray findings further define SCFE etiology.

Keywords: functional classification of genes; microarray; slipped capital femoral epiphysis (SCFE)

Corresponding author: William J. Landis, PhD, Department of Polymer Science, Goodyear Polymer Center, Room 1201C, The University of Akron, 170 University Avenue, Akron, OH 44325-3909, USA, Phone: +1-330-972-8483, Fax: +1-330-972-5920

Acknowledgments

This study would not have been possible without the services for microarray analysis provided by the late Mr. David Newsom of the Biomedical Genomics Core at The Research Institute of Nationwide Children’s Hospital, Columbus, OH, USA. The authors are also grateful for the help with patient consents and sample collection provided by Ms. Debbie Hawk, RN, CNOR, at the Akron Children’s Hospital.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This study was supported in part by donations from the Women’s Board of Akron Children’s Hospital specifically for orthopaedic research and education (to DSW) and by a grant from the Northeast Ohio Medical University, Rootstown, Ohio (to WJL).
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2016-1-14

Accepted: 2016-5-9

Published Online: 2016-7-8

Published in Print: 2016-8-1

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Articles in the same Issue

https://doi.org/10.1515/jpem-2016-0023

Keywords for this article

functional classification of genes; microarray; slipped capital femoral epiphysis (SCFE)