The protective effect of hydrolyzed collagen gel from mackerel scad (Decapterus macarellus) to attenuate chronic UVB-induced photodamage
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Elisa Herawati
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Rizki S. Titisari
Abstract
Objectives
Hydrolyzed collagen (HC) extracted from fish skin is a safe and effective native collagen alternative that can potentially protect skin from experiencing photoaging. This study is among the first to explore HC from mackerel scad (Decapterus macarellus) application via in vitro and in vivo approaches.
Methods
The protective effects of HC were investigated on Mouse Embryonic Fibroblasts (MEFs) induced by acute and chronic UVB, followed by testing of HC gel on UVB-induced photoaging models of mice. Cell viability test using the MTT method was performed on UVB-induced MEFs treated with HC concentrations of 10, 20, 50, and 100 g/mL. A gel form HC was applied topically in UVB-induced skin tissues of animal models and analyzed with ELISA to evaluate the inhibitory effect of MMP-1 and IL-6 expression.
Results
In vitro analysis showed that HC (10, 20, 50 μg/mL) could increase the viability of acute UVB-induced MEFs at a 60 mJ/cm2 dose. In chronic UVB exposure, all HC concentrations could protect the viability of induced MEFs at 40 mJ/cm2 dose. HC gel effectively inhibited the increased expression of MMP-1 and IL-6 in UVB-exposed skin, which may be related to its ability to suppress the activation of MAPK and NF-κB signaling pathways. Furthermore, HC gel intervened in the development of epidermal thickening and dermal layer damage in photoaged skin.
Conclusions
These findings indicated that HC from mackerel scad skin effectively provided a protective effect against the photoaging process.
Acknowledgments
The authors would like to thank Vicky Alvino Setyawan for helping in hydrolyzed collagen extraction and mice handling.
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Research ethics: The animal study protocol was approved by the Health Research Ethics Committee of Faculty of Medicine, Muhammadiyah Surakarta University (protocol code: (i) for acute UVB exposure: 3395/A.1/KEPK-FKUMS/III/2021; issued on March, 10th 2021, (ii) for chronic UVB exposure: 4152/A.1/KEPK-FKUMS/III/2022; issued on March, 21st 2022), and (iii) for anti-photoaging bioactivity in vivo study: 03/UN27.06.11/KEP/EC/2023.
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Informed consent: Not applicable.
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Author contributions: Conceptualization, E.H., H.K. R.S.T; methodology, E.H. H.K., F.F.T.A.P., R.S.T., S.L., O.P.A.; data curation, H.K., R.S.T., F.F.T.A.P., E.H.; writing – original draft preparation, E.H., H.K., R.S.T; writing – review and editing, P.A., E.H.; supervision, E.H., S.L., O.P.A.; project administration, E.H.; funding acquisition, E.H., E.S., N.H., N.L., F.I. All authors have read and agreed to the published version of the manuscript.
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Use of Large Language Models, AI and Machine Learning Tools: None declared.
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Conflict of interest: The authors declare no conflict of interest. The funder had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
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Research funding: This research was funded by Universitas Sebelas Maret, grant number 590.1/UN27.22/HK.07.00/2023.
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Data availability: The data presented in this study are available on request from the corresponding author.
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