Scopoletin a potential phytochemical therapy for antitubercular treatment drug induced liver injury (ATT-DILI) model in Wistar rats

Swati Sharma; Vishal Sharma; Sunil Taneja; Alka bhatia; Aishwarya Anand; Amol N. Patil; Dibyajyoti Banerjee

doi:10.1515/jcim-2023-0168

Article

Scopoletin a potential phytochemical therapy for antitubercular treatment drug induced liver injury (ATT-DILI) model in Wistar rats

Swati Sharma , Vishal Sharma , Sunil Taneja , Alka bhatia , Aishwarya Anand , Amol N. Patil and Dibyajyoti Banerjee

Published/Copyright: September 22, 2023

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From the journal Journal of Complementary and Integrative Medicine Volume 20 Issue 4

Abstract

Objectives

The hepatoprotective properties of scopoletin have been explored in carbon tetrachloride (CCl4) induced liver injury but not in drug-induced liver injury (DILI) scenarios. Only N-acetyl-cysteine (NAC) has proven efficacy in DILI treatment. Accordingly, we conducted a study to assess the hepatoprotective action of scopoletin in the anti-tubercular treatment (ATT)-DILI model in Wistar rats, if any.

Methods

A total of 36 rats were evaluated, with six in each group. A 36-day ATT at 100 mg/kg dose for isoniazid, 300 mg/kg for rifampicin and 700 mg/kg for pyrazinamide were fed to induce hepatotoxicity in rats. Group I and II–VI received normal saline and ATT, respectively. Oral scopoletin (1,5 and 10 mg/kg) and NAC 150 mg/kg were administered in groups III, IV, V and VI, respectively, once daily for the last 15 days of the experiment. LFT monitoring was performed at baseline, days 21, 28, and 36. Rats were sacrificed for the histopathology examination.

Results

Aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and bilirubin levels were significantly increased in group II (receiving ATT) compared to normal control on day 28 and day 36 (p<0.05). All three doses of scopoletin and NAC groups led to the resolution of AST, ALT, ALP, and bilirubin changes induced by ATT medications effect beginning by day 28 and persisting on day 36 (p<0.01). An insignificant effect was observed on albumin and total protein levels. The effect was confirmed with antioxidants and histopathology analysis.

Conclusions

The study confirms the hepatoprotective efficacy of scopoletin in a more robust commonly encountered liver injury etiology.

Keywords: Ayurveda; phytochemical; Indian traditional medicine; liver injury; drug induced liver injury; antitubercular treatment; scopoletin

Corresponding author: Dr Amol N. Patil, Associate Professor, Department of Pharmacology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh 160012, India, Phone: +91 9990245973, 7087008957 28, E-mail: patil.amol@pgimer.edu.in

Ethical approval: Study protocol was approved by the Animal Ethics Committee (761/IAEC/110-D) and all experiments complied with the ARRIVE (Animal Research: Reporting of In Vivo Experiments) 2.0 guidelines.
Informed consent: Informed consent was not needed in this study.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Research funding: None declared.
Data availability: Raw data can be availed from the corresponding author upon reasonable request.

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Received: 2023-06-13

Accepted: 2023-08-28

Published Online: 2023-09-22

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Articles in the same Issue

https://doi.org/10.1515/jcim-2023-0168

Keywords for this article

Ayurveda; phytochemical; Indian traditional medicine; liver injury; drug induced liver injury; antitubercular treatment; scopoletin