Gastroprotective and ulcer healing effects of Nauclea pobeguinii (Rubiaceae) on experimentally induced gastric ulcers in male Wistar rats

Karelle Kouam Yacine Madjo; Marius Mbiantcha; Linda Zenab Njoya Fagni; Vanessa Mba Matah Marthe; Elvira Azanze Ngoufack; Gonzal Eric Tsafack; Stephanie Flore Djuichou Nguemnang; Carine Flore Adjouzem; Gilbert Ateufack

doi:10.1515/jcim-2023-0024

Article

Gastroprotective and ulcer healing effects of Nauclea pobeguinii (Rubiaceae) on experimentally induced gastric ulcers in male Wistar rats

Karelle Kouam Yacine Madjo , Marius Mbiantcha , Linda Zenab Njoya Fagni , Vanessa Mba Matah Marthe , Elvira Azanze Ngoufack , Gonzal Eric Tsafack , Stephanie Flore Djuichou Nguemnang , Carine Flore Adjouzem and Gilbert Ateufack

Published/Copyright: June 30, 2023

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From the journal Journal of Complementary and Integrative Medicine Volume 20 Issue 4

Abstract

Objectives

In this study, we determined the gastroprotective and ulcer-healing effects of extracts (aqueous and methanolic) of Nauclea pobeguinii stem-back.

Methods

Gastroprotective and healing activity were evaluated following a HCl/ethanol and an indomethacin-induced acute ulcers models; acetic acid, pylorus-ligature, pylorus ligature/histamine and pylorus ligature/acetylcholine-induced chronic ulcers models.

Results

It emerges from this study that, at 100, 200 and 400 mg/kg, the extracts significantly reduced the various ulceration parameters. Compared to negative control male rats, the aqueous (100 mg/kg) and methanolic (400 mg/kg) extracts of Nauclea pobeguinii inhibited the ulcers induced by HCl/ethanol by 80.76 % and 100 % respectively, as well as ulcers induced by indomethacin by 88.28 % and 93.47 % respectively. Animals that received 200 mg/kg of both extracts showed a significant reduction in the levels of monocytes, lymphocytes, nitric oxide, MDA and a significant increase in the activities of SOD and catalase. Histological analysis showed repaired mucous epithelium at all doses of both extracts. Aqueous and methanol extracts inhibited ulceration indices by 89.33 % and 88.53 % for pylorus ligature, 83.81 % and 61.07 % for pylorus ligature/acetylcholine and 87.29 % and 99.63 % for pylorus ligature/histamine respectively. Both extracts protected the stomach lining with percentages inhibition of 79.49 % and 81.73 %, respectively in the ethanol test. The extracts induced a significant increase in mucus mass (p<0.001).

Conclusions

The aqueous and methanol extracts of Nauclea pobeguinii healed ulcers thanks to their anti-inflammatory, anti-oxidant, anti-secretory and cytoprotective properties.

Keywords: gastric ulcers; gastroprotective; Nauclea pobeguinii ; ulcer healing

Corresponding authors: Prof. Marius Mbiantcha and Prof. Gilbert Ateufack, Faculty of Science, Laboratory of Animal Physiology and Phytopharmacology, University of Dschang, P.O. Box 67, Dschang, Cameroon, Phone: +00237 699034998, E-mail: mbiantchamarius@yahoo.fr, Phone: +00237 675277614, E-mail: ateufack2000@yahoo.fr

Research funding: None declared.
Author contributions: MM, AG and MKYK designed the work. MKYK, AG, MM, FZNL, NAE, TEG, DNSF, ACF and MMVM collected and analysed the data. MM, MKYK and AG drafted, read and revised critically the manuscript. All authors accept to be accountable for all aspects of the work.
Competing interests: The authors declare no conflicts of interest regarding this article.
Informed consent: Not applicable.
Ethical approval: All the procedures described in this document (use of animals for experimentation) were in absolute respect for ethics, the preservation, breeding and handing of the fauna and flora of Cameroon (Ethics Committee of the Cameroonian Ministry of Scientific Research and Technology) adopted the guidelines (European Union on animal care and testing) (Council CCE 86/609).
Data availability: All data supporting our findings are adequately contained within the manuscript.

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Received: 2023-01-26

Accepted: 2023-06-15

Published Online: 2023-06-30

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Articles in the same Issue

https://doi.org/10.1515/jcim-2023-0024

Keywords for this article

gastric ulcers; gastroprotective; Nauclea pobeguinii; ulcer healing