Evaluation of in-vitro and in-vivo antidiabetic, antilipidemic and antioxidant potentials of aqueous root extract of Strophanthus hispidus DC (Apocynaceae)
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Muyiwa Samuel Fageyinbo
, Abidemi James Akindele
Abstract
Background
Antidiabetic activity of aqueous root extract of Strophanthus hispidus (SHP) was evaluated based on its folklore used in traditional medicine for the treatment of diabetes. Objectives: This study aimed to investigate the in-vitro and in-vivo antidiabetic potential of the aqueous root extract of SHP.
Methods
SHP (50, 100 and 200 mg/kg p.o.), glibenclamide (5 mg/kg p.o.), normal saline (10 mL/kg; diabetic control) and distilled water (10 mL/kg; normal control) were administered once daily for 28 days, with the measurement of fasting blood glucose level at 7 days interval. Blood samples were collected on day 28 for serum biochemical (albumin, total protein [TP], creatinine, alanine transaminase [ALT], aspartate transaminase [AST], alkaline phosphatase [ALP], triglycerides [TG], total cholesterol [TC], high-density lipoprotein [HDL], low-density lipoprotein [LDL], bilirubin and urea) and hematological assays. The in-vitro antidiabetic activity was investigated using α-amylase and α-glucosidase enzymes inhibitory assays.
Results
SHP produced a day-dependent reduction in glucose level. Peak reduction (82.94 %; p < 0.05) was produced at the dose of 100 mg/kg. SHP significantly (p < 0.05) increased the level of HDL and TP but significantly (p < 0.05) reduced the levels of TG, LDL, TC, AST, ALT, ALP, bilirubin, creatinine and urea compared with diabetic control rats. Furthermore, SHP significantly (p < 0.05) increased the level of catalase, superoxide dismutase and reduced glutathione compared to diabetic control rats. SHP significantly (p < 0.05) inhibited α-amylase and α-glucosidase enzymes compared with acarbose.
Conclusion
The findings in this study showed that SHP possesses beneficial antidiabetic activity.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report or in the decision to submit the report for publication.
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Artikel in diesem Heft
- Review
- A review on traditional practice of tooth brushing in Ayurveda and its relevance in current era
- Dietary antioxidant for disease prevention corroborated by the Nrf2 pathway
- Mini Review
- Effects of dietary supplementations and herbs on migraine – a systematic review
- Pre-clinical studies
- Assessment of compliance level of ICH guidelines for organic volatile impurities in common ayurvedic hepatic formulations
- Nephroprotective properties of the methanol stem extract of Abrus precatorius on gentamicin-induced renal damage in rats
- Phytochemical analysis, cytotoxic and antioxidant activities of Myrtus communis essential oil from Morocco
- In vitro and in vivo toxicity assessment of biologically synthesized silver nanoparticles from Elaeodendron croceum
- Evaluation of in-vitro and in-vivo antidiabetic, antilipidemic and antioxidant potentials of aqueous root extract of Strophanthus hispidus DC (Apocynaceae)
- Clinical studies
- Effects of acupuncture on chronic idiopathic pruritus: an uncontrolled pilot study evaluating inflammatory changes with treatment
- Medicinal plants’ use among patients with dyslipidemia: an Iranian cross-sectional survey
- Music to reduce pain and anxiety in cystoscopy: a systematic review and meta-analysis
- Fenugreek dermal patch, a new natural topical antinociceptive medication for relieving the postherniotomy pain, a double-blind placebo controlled trial
- Evaluating the effect of lifestyle education based on peer model on anxiety in patients with acute myocardial infarction
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