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Nephroprotective properties of the methanol stem extract of Abrus precatorius on gentamicin-induced renal damage in rats

  • Olufunke Olubunmi Falayi , Ademola Adetokunbo Oyagbemi , Temidayo Olutayo Omobowale , Emmanuel Abiodun Ayodele , Aduragbenro Deborah Adedapo , Momoh Audu Yakubu and Adeolu Alex Adedapo EMAIL logo
Published/Copyright: October 26, 2018
Journal of Complementary and Integrative Medicine
From the journal Journal of Complementary and Integrative Medicine Volume 16 Issue 3

Abstract

Background

The use of plants for the treatment and prevention of diseases in man and his animals has led to a renewed scientific interest in the use of medicinal plants for therapeutic purposes. The nephroprotective properties of methanol stem bark extract of Abrus precatorius against gentamicin-induced renal damage in rats was evaluated in this study.

Methods

Thirty male Wistar rats were divided into five equal groups. Group A was the negative control group while B was the positive control group which received gentamicin 100 mg/kg intra-peritoneally for 6 days. Group C were pretreated with 100 mg/kg extract for the 3 days and then concurrently with gentamicin 100 mg/kg for 3 days and group D were pretreated with 200 mg/kg extract for 3 days and then concurrently with gentamicin 100 mg/kg for 3 days. Group E received gentamicin intraperitoneally for 6 days followed by administration of 200 mg/kg of the extract for 3 days. Blood samples, kidneys and kidney homogenates were collected for haematological, biochemical, histopathological and immunohistochemical analysis.

Results

The results showed that no significant haematological changes were noted. The groups treated with extract exhibited significant increase in body weight gain. While group B significantly exhibited focal areas of inflammation, fatty degeneration, congestion of vessels, tubular necrosis and glomerular atrophy, the lesions were mild with the treated groups. Treated groups exhibited a dose dependent significant decrease in serum creatinine, urea, XO, NO and Myeloperoxidase, AOPP, Protein carbonyl, H2O2 generated and MDA levels when compared with group B. There were significant dose dependent improvements in SOD, GST, GSH, Protein thiol, and non-protein thiol levels in the treated groups when compared with group B. In immunohistochemistry, Group B exhibited over expression of CRP and NF-κB levels, and marked reduction in expression of Bcl-2 while the reverse was seen in the groups treated with methanol extracts of Abrus precatorius.

Conclusion

The methanol extract of Abrus precatorius plays a vital role against gentamicin induced renal damage by reducing levels of renal markers of oxidative stress, inflammation and apoptosis, enhancing enzymatic and non enzymatic renal antioxidant system, alongside an increase in Bcl-2 and a decrease in NF-κB and CRP expressions.

Keywords: Abrus precatorius; anti-oxidant; gentamicin; immunohistochemistry; nephroprotection

Acknowledgments

The authors acknowledged that the study was supported with a grant received from the National Research Foundation of the Tertiary Education Trust Fund (TETFUND), Abuja, Nigeria. The grant number is TETFUND/DESS/NRF/UI IBADAN/STI/VOL/I/B2.20.11.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: National Research Foundation of the Tertiary Education Trust Fund (TETFUND), Abuja, Nigeria (TETFUND/DESS/NRF/UI IBADAN/STI/VOL/I/B2.20.11)

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Correction-note

Correction added after ahead-of-print publication on October 26, 2018: The misprint for several instances of Bcl2, NFKB and H2O2 has been amended throughout the entire article.

Received: 2017-12-20
Accepted: 2018-09-13
Published Online: 2018-10-26

© 2019 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jcim-2017-0176
Keywords for this article
Abrus precatorius; anti-oxidant; gentamicin; immunohistochemistry; nephroprotection

Articles in the same Issue

  1. Review
  2. A review on traditional practice of tooth brushing in Ayurveda and its relevance in current era
  3. Dietary antioxidant for disease prevention corroborated by the Nrf2 pathway
  4. Mini Review
  5. Effects of dietary supplementations and herbs on migraine – a systematic review
  6. Pre-clinical studies
  7. Assessment of compliance level of ICH guidelines for organic volatile impurities in common ayurvedic hepatic formulations
  8. Nephroprotective properties of the methanol stem extract of Abrus precatorius on gentamicin-induced renal damage in rats
  9. Phytochemical analysis, cytotoxic and antioxidant activities of Myrtus communis essential oil from Morocco
  10. In vitro and in vivo toxicity assessment of biologically synthesized silver nanoparticles from Elaeodendron croceum
  11. Evaluation of in-vitro and in-vivo antidiabetic, antilipidemic and antioxidant potentials of aqueous root extract of Strophanthus hispidus DC (Apocynaceae)
  12. Clinical studies
  13. Effects of acupuncture on chronic idiopathic pruritus: an uncontrolled pilot study evaluating inflammatory changes with treatment
  14. Medicinal plants’ use among patients with dyslipidemia: an Iranian cross-sectional survey
  15. Music to reduce pain and anxiety in cystoscopy: a systematic review and meta-analysis
  16. Fenugreek dermal patch, a new natural topical antinociceptive medication for relieving the postherniotomy pain, a double-blind placebo controlled trial
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