Antidiabetic and protective effects of the aqueous extract of Arbutus unedo L. in streptozotocin-nicotinamide-induced diabetic mice
-
Hanae Naceiri Mrabti
, Karima Sayah
Abstract
Background
Diabetes mellitus (DM) is currently a major health problem and the most common chronic disease worldwide. Traditional medicinal plants remedies remain a potential adjunct therapy to maintain better glycemic control while also imparting few side-effects. Arbutus unedo L. has been traditionally used to manage several diseases including diabetes. This study was undertaken to contribute the validation of the traditional use of Arbutus unedoL. (Ericaceae) in the treatment of diabetes.
Methods
In-vitro antidiabetic effect of the A. unedo roots aqueous extract was conducted using α-glucosidase and α-amylase assays. While in-vivo antidiabetic activity was conducted using streptozotocin-nicotinamide (STZ-NA) induced diabetic mice. Diabetic animals were orally administered the aqueous extract in 500 mg/kg of body weight to assess the antidiabetic effect. The blood glucose level and body weight of the experimental animals were monitored for 4 weeks. In addition, the histopathological examination of the treated mice pancreas was also conducted to observe the changes of β-cells during the treatment process.
Results
The extract produced a significant decrease in blood glucose level in diabetic mice. This decrease was equivalent to that which observed in mice treated with a standard after 2–4 weeks. In addition, the plant extract exhibited a potent inhibitory effect on α-amylase and α-glucosidase activity with IC50 values of 730.15±0.25 μg/mL and 94.81±5.99 μg/mL, respectively. Moreover, the histopathologic examination of the pancreas showed a restoration of normal pancreatic islet cell architecture which observed in the diabetic mice treated with plant extract.
Conclusions
The aqueous A. unedo roots extract has a significant in vitro and in vivo antidiabetic effects and improves metabolic alterations. The revealed results justify its traditional medicinal use.
Acknowledgments
The authors are thankful to Wessal Ouedrhiri (National Agency of Medicinal and Aromatic Plants, University Sidi Mohamed Ben Abdellah, BP 159, Taounate, Morocco) for her support technique in the paper redaction, and to Pr. Jamal ElOualidi (Botanical Department and ecology vegetal of the Scientific Institute of Rabat, Morocco) for plants authentication.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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© 2018 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Review
- Active phytoconstituents for diabetes management: A review
- Pharmacological potentials of betalains
- The effects of fennel on menstrual bleeding: A systematic review and meta-analysis
- Use of natural products for oral hygiene maintenance: revisiting traditional medicine
- Antimicrobial properties of terrestrial snail and slug mucus
- Historical and cross-cultural perspectives on Parkinson’s disease
- The effects of Sahaja Yoga meditation on mental health: a systematic review
- Preclinial Studies
- Antidiabetic and protective effects of the aqueous extract of Arbutus unedo L. in streptozotocin-nicotinamide-induced diabetic mice
- Phytochemical profile, antimicrobial, antioxidant and antiobesity activities of Scolymus angiospermus Gaertn. Four fractions from Jericho/Palestine
- Evaluating of β-carotene role in ameliorating of favism-induced disturbances in blood and testis
- Subchronic oral toxicity study of Habb-e-Suranjan in albino Wistar rats
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- Clinical Studies
- Beneficial effect of ghee consumption over mustard oil on lipid profile: A study in North Indian adult population
- Diet enriched with fresh coconut decreases blood glucose levels and body weight in normal adults
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- Is fish oil supplementation effective on maternal serum FBS, oral glucose tolerance test, hemoglobin and hematocrit in low risk pregnant women? A triple-blind randomized controlled trial
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