Dendrobium chrysanthum ethanolic extract induces apoptosis via p53 up-regulation in HeLa cells and inhibits tumor progression in mice
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Ritika Prasad
Abstract
Background
Dendrobium is one of the diverse genus of orchid plants. It possesses a number of pharmacological activities and has long been used in traditional system of medicine. The goal of this study was to investigate the apoptosis inducing property of the ethanolic extract from the leaves of Dendrobium chrysanthum, a species of Dendrobium whose anticancer role has not been ascertained yet.
Methods
To evaluate the anticancer activity of the ethanolic extract of D. chrysanthum in vitro in HeLa (human cervical cancer) cells, cytotoxic activity, generation of reactive oxygen species (ROS), induction of apoptosis and effect on cell cycle were determined. The in vivo study was carried out in Dalton’s lymphoma (DL) bearing mice to assess the tumor growth delay.
Results
Our study demonstrated that the ethanolic extract showed dose-dependent cytotoxicity against HeLa cells. The extract exhibited dose-dependent increase in ROS production as well as apoptotic cell death which was further confirmed through presence of DNA fragmentation. Cell cycle analysis by flow cytometry suggests that the ethanolic extract perturbed cell cycle progression and leads to the delay of the cells in S phase. Further, the real-time PCR studies also showed up-regulation of apoptotic genes p53 and Bax. The in vivo antitumor activity exhibited significant increase in the life span of DL bearing mice as compared to control with significant decrease in abdominal size along with reduced tumor ascites.
Conclusions
These observations demonstrate the anticancer potential of the D. chrysanthum ethanolic extract mediated through p53-dependent apoptosis.
Acknowledgments
The authors kindly acknowledge the Interdisciplinary school of Life sciences (ISLS), Institute of Science, Banaras Hindu University, Varanasi for providing us the flow cytometry (FACS) and Real-time PCR facility and to the Head, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi for providing the infrastructural facility.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: The author(s) disclosed receipt of the following financial support for the research and/or authorship of this article: The financial support from University Grant Commission (UGC), New Delhi, India [F. No. 41-162/2012 (SR)] to carry out this study is gratefully acknowledged. The author is also thankful to UGC, New Delhi for RFSMS, Senior research fellowship.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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© 2017 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Review
- Ethnopharmacological approaches in mood and anxiety disorders. The relevance of the oxidative stress status
- Preclinical Studies
- Cytokine changes in gastric and colonic epithelial cell in response to Planta ovata extract
- Preliminary phytochemical analysis and evaluation of antioxidant, cytotoxic and inhibition of lipopolysaccaride - induced NOS (iNOS) expression in BALB/c mice liver by Ziziphus oenoplia Mill. fruit
- Evaluation of the sub-chronic toxicity profile of the corm of Xanthosoma sagittifolium on hematology and biochemistry of alloxan-induced diabetic Wistar rats
- Dendrobium chrysanthum ethanolic extract induces apoptosis via p53 up-regulation in HeLa cells and inhibits tumor progression in mice
- Safety assessment of the aqueous extract of the flowers of Nymphaea lotus Linn (Nymphaeaceae): Acute, neuro- and subchronic oral toxicity studies in albinos Wistar rats
- Effect of aqueous extract of Aegle marmelos fruit and leaf on glycemic, insulinemic and lipidemic status of type 2 diabetic model rats
- Antihypernociceptive and antioxidant effects of Petersianthus macrocarpus stem bark extracts in rats with complete Freund’s adjuvant-induced persistent inflammatory pain
- ROS-mediated induction of apoptosis by benzoquinone embelin in human colon adenocarcinoma cells HT-29
- Clinical Studies
- Desensitization to chemical and food sensitivities by low-dose immunotherapy ascertained by provocation neutralization is associated with reduced influx of calcium ions into lymphocytes
- Managing irritable bowel syndrome: The impact of micro-physiotherapy
- Short Communication
- Design development and evaluation of triphala tablets
