A comparative study of the antacid effect of raw spinach juice and spinach extract in an artificial stomach model
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Abstract
Background
Spinacia oleracea known as spinach is a green-leafy vegetable consumed by people across the globe. It is reported to possess potent medicinal properties by virtue of its numerous antioxidant phytoconstituents, together termed as the natural antioxidant mixture (NAO). The present study compares the antacid effect of raw spinach juice with an antioxidant-rich methanolic extract of spinach (NAOE) in an artificial stomach model.
Methods
The pH of NAOE at various concentrations (50, 100 and 200 mg/mL) and its neutralizing effect on artificial gastric acid was determined and compared with that of raw spinach juice, water, the active control sodium bicarbonate (SB) and a marketed antacid preparation ENO. A modified model of Vatier’s artificial stomach was used to determine the duration of consistent neutralization of artificial gastric acid for the test compounds. The neutralizing capacity of test compounds was determined in vitro using the classical titration method of Fordtran.
Results
NAOE (50, 100 and 200 mg/mL), spinach juice, SB and ENO showed significantly better acid-neutralizing effect, consistent duration of neutralization and higher antacid capacity when compared with water. Highest antacid activity was demonstrated by ENO and SB followed by spinach juice and NAOE200. Spinach juice exhibited an effect comparable to NAOE (200 mg/mL).
Conclusions
Thus, it may be concluded that spinach displays significant antacid activity be it in the raw juice form or as an extract in methanol.
Author contributions
All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report or in the decision to submit the report for publication.
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©2016 by De Gruyter
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Articles in the same Issue
- Frontmatter
- Review
- New horizon in the treatment of sepsis: a systematic review of alternative medicine
- Medical Education
- Medical student attitudes towards complementary and alternative medicine (CAM) in medical education: a critical review
- Advancing medical education: connecting interprofessional collaboration and education opportunities with integrative medicine initiatives to build shared learning
- Preclinical Studies
- Anti-adenovirus activity, antioxidant potential, and phenolic content of black tea (Camellia sinensis Kuntze) extract
- Biochemical fingerprint and pharmacological applications of Barleria noctiflora L.f. leaves
- Alleviation of 5-fluorouracil-induced intestinal mucositis in rats by vitamin E via targeting oxidative stress and inflammatory markers
- A comparative study of the antacid effect of raw spinach juice and spinach extract in an artificial stomach model
- Diuretic activity and toxicity study of the aqueous extract of Cola nitida seed on markers of renal function and electrolytes in rats
- Clinical Studies
- The effects of the Bali Yoga Program (BYP-BC) on reducing psychological symptoms in breast cancer patients receiving chemotherapy: results of a randomized, partially blinded, controlled trial
- DLBS3233, a combined bioactive fraction of Cinnamomum burmanii and Lagerstroemia speciosa, in type-2 diabetes mellitus patients inadequately controlled by metformin and other oral antidiabetic agents
- Online Only: Congress Abstracts (available at DOI:http://dx.doi.org/10.1515/jcim-2016-0102)
- 2016 INCAM Research Symposium: Expanding Person-Centred Care through Integrative Health Research
