Modulatory action of Moringa oleifera Lam. on L-arginine induced acute pancreatitis

Tosan Peter Omayone; Omamuyovwi Meashack Ijomone; Solomon Babatunde Oloyede; Salihaat Toyin Okunola; Zainab Oluwabukola Aigoro; Victory Uwuma Esukpa; Samuel Oluwaseun Dinakin

doi:10.1515/jbcpp-2021-0149

Article

Modulatory action of Moringa oleifera Lam. on L-arginine induced acute pancreatitis

Tosan Peter Omayone , Omamuyovwi Meashack Ijomone , Solomon Babatunde Oloyede , Salihaat Toyin Okunola , Zainab Oluwabukola Aigoro , Victory Uwuma Esukpa and Samuel Oluwaseun Dinakin

Published/Copyright: October 4, 2021

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From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 34 Issue 6

Abstract

Objectives

Acute pancreatitis (AP) is an inflammatory disease of the pancreas with high morbidity and mortality. This study investigates the effect of Moring oleifera (MO) on L-arginine-induced AP in Wistar rats.

Methods

Male Wistar rats were randomly divided into seven groups. Control, AP, Magnesium groups, all fed with standard rat diet, MO leaf groups (5% MLF and 15% MLF), and MO seed groups (5% MSD and 15% MSD) were fed with five or 15% MO leaf or seed supplemented diet for four weeks prior to induction of AP. AP was induced by administration of double doses of L-arginine (320 mg/100 g i.p.) at 1 h interval. All animals were sacrificed 72 h thereafter.

Results

Weekly mean feed consumption and body weight were significantly higher in MO groups compared to the control. Amylase level, MDA, MPO, and NO were significantly higher in the AP group than in the control but decreased in Mg and MO groups. While CAT, SOD, GSH, and SH-group were significantly depleted in AP groups, which was attenuated in MO groups. Rats in AP groups showed severe inflammation, necrosis, and edema. These effects were significantly improved in MO groups resulting in lower histological scores compared to the AP group.

Conclusions

Pretreatment with MO could attenuate AP via its antioxidant and anti-inflammatory action.

Keywords: acute pancreatitis; amylase; antioxidant; inflammation; Moringa oleifera

Corresponding author: Dr. Tosan Peter Omayone, Department of Physiology, School of Health and Health Technology, Federal University of Technology Akure, Akure, Nigeria, Phone: +07039732960, E-mail: tpomayone@futa.edu.ng

Acknowledgments

The authors acknowledge Mr. Seyi Olabanji Aleruwa and Mr. Ismail Akanni Oguntade (technologist at the Department of Physiology, Federal University of Technology Akure) for their technical assistance during the experimental procedures. The authors are also grateful to Miss Comfort Okoh (of The Neuro- Lab, SHHT, FUTA) for the technical assistance with histological analysis.

Research Funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-public sectors.
Competing interests: The authors declare no conflict of interest.
Ethical approval: The research has complied with the criteria outlined in the Guide for the Care and Use of Laboratory Animals prepared by the National Academy of Science, 2011.
Author contributions: Author Omayone TP conceive the idea and design the work. Data acquisition was done by authors Solomon Babatunde Oloyede, Salihaat Toyin Okunola, Zainab Oluwabukola Aigoro, Victory Uwuma Esukpa, and Samuel Oluwaseun Dinakin under the supervision of author Omayone TP. All authors were involved in data analysis and interpretation. The first draft of the manuscript was written by authors Omayone TP, Solomon Babatunde Oloyede, Salihaat Toyin Okunola and revised by Omamuyovwi Meashack Ijomone. Final approval of the version to be published was decided by all authors and all authors have accepted responsibility for the entire content of this manuscript and approved its submission.

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Received: 2021-05-18

Accepted: 2021-09-08

Published Online: 2021-10-04

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Articles in the same Issue

https://doi.org/10.1515/jbcpp-2021-0149

Keywords for this article

acute pancreatitis; amylase; antioxidant; inflammation; Moringa oleifera