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Biochemical and histopathological effects of low dose vanadium in the healing of acetic acid-induced colitis in male wistar rats

  • Tosan Peter Omayone EMAIL logo and Samuel Babafemi Olaleye EMAIL logo
Published/Copyright: February 15, 2021
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 33 Issue 3

Abstract

Objectives

Vanadium has been reported to possess relevant therapeutic properties such as anti-diabetic and anti-tumoral. This study aimed at determining the effects of vanadium on experimentally induced colitis in rats.

Methods

Forty-five male Wistar rats (103 ± 3.90 g, n=15) were used for this study and were divided into three groups. Group 1 (Untreated control) had nothing added to their drinking, while groups 2 and 3 received sodium metavanadate at a dose of 50 and 200 mg/L respectively in their drinking water for 10 weeks. Colitis was thereafter induced by intra colonic administration of 1.50 mL of 6% acetic acid. Animals were sacrificed on day 0 (pre-induction), three- and seven-days post induction. Blood samples were collected for haematological variables and the distal 8 cm of the colon was collected for macroscopic, histological and biochemical (malondialdehyde-MDA, superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase- GPx and nitrite concentration- NO) assessment.

Results

Low dose vanadium proved beneficial in ameliorating acetic acid-induced colitis by improving both histopathological and haematological changes. Gross observation showed a faster healing rate in vanadium treated groups (50 and 200 mg/L) compared with untreated control at day 3 (40 and 26.20 vs. 2.50%) and day 7 (80 and 66.70 vs. 42%) respectively. Vanadium also appears to exert its beneficial effects on acetic acid-induced colitis via up regulation of antioxidant enzymes (SOD, CAT, GPx) and NO while decreasing the over production of MDA.

Conclusions

Vanadium at small concentration functions as an essential trace element and may be able to promote healing process during ulcerative colitis.

Keywords: histomorphometry; inflammatory bowel disease; oxidative stress; sodium metavanadate; ulcerative colitis
Corresponding authors: Dr. Tosan Peter Omayone, Gastrointestinal Secretion and Inflammation Research Unit, Department of Physiology, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Oyo State, Nigeria; and Department of Physiology, School of Health and Health Technology, Federal University of Technology Akure, Akure, Nigeria, Phone: 07039732960, E-mail: ; and Prof. Samuel Babafemi Olaleye, Gastrointestinal Secretion and Inflammation Research Unit, Department of Physiology, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Oyo State, Nigeria, Phone: 08023255893, E-mail:

Acknowledgement

The authors are grateful to Dr John Ogunsola of the Department of Veterinary Medicine, University of Ibadan, Nigeria for the interpretation of the histological slides.

  1. Research funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-public sectors.

  2. Author contributions: Author Olaleye SB and Omayone TP both conceive the idea and design the work. Data acquisition was done by author Omayone TP while, author Olaleye SB supervised the work. Both authors were involved in data analysis and interpretation. First draft of the manuscript was written by author Omayone TP and revised by Olaleye SB. Final approval of the version to be published was decided by both authors and both authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: The research has complied with the criteria outlined in the Guide for the Care and Use of Laboratory Animals prepared by the National Academy of Science, 2011.

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Received: 2020-08-17
Accepted: 2020-10-31
Published Online: 2021-02-15

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. An insight on safety, efficacy, and molecular docking study reports of N-acetylcysteine and its compound formulations
  4. Public health approach of Unani medicine to cope and stay safe in hot environmental conditions
  5. Original Articles
  6. Kinetic characterization of rat brain acetylcholinesterase modulated by lead and cartap: the ameliorative effect of Citrus limon fruit juice
  7. Attenuation of potassium dichromate and sodium arsenite toxicities by methanol extract of Rauvolfia vomitoria in mice
  8. Chronic exposure of industrial grade calcium carbide and ethylene glycol alter histological architecture of systemic organs by disrupting redox balance in rat
  9. Biochemical and histopathological effects of low dose vanadium in the healing of acetic acid-induced colitis in male wistar rats
  10. Glycine and L-Arginine supplementation ameliorates gastro-duodenal toxicity in a rat model of NSAID (Diclofenac)-gastroenteropathy via inhibition of oxidative stress
  11. Cocos nucifera L. oil alleviates lead acetate-induced reproductive toxicity in sexually-matured male Wistar rats
  12. Intracellular signaling molecules of nerve tissue progenitors as pharmacological targets for treatment of ethanol-induced neurodegeneration
  13. Glatiramer acetate treatment inhibits inflammatory responses and improves survival in a mice model of cecal ligation and puncture-induced sepsis
  14. Ciprofloxacin-induced cutaneous adverse drug events: a systematic review of descriptive studies
  15. Aqueous extract of Adansonia digitata prevents Cadmium Chloride-induced testicular damage in Wistar Rats
  16. Exposure to prolonged unpredictable light impairs spatial memory via induction of oxidative stress and tumor necrosis factor-alpha in rats
  17. Tamarindus indica. Linn leaves ameliorates experimental induced heart failure in Wistar rats
https://doi.org/10.1515/jbcpp-2020-0246
Keywords for this article
histomorphometry; inflammatory bowel disease; oxidative stress; sodium metavanadate; ulcerative colitis

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. An insight on safety, efficacy, and molecular docking study reports of N-acetylcysteine and its compound formulations
  4. Public health approach of Unani medicine to cope and stay safe in hot environmental conditions
  5. Original Articles
  6. Kinetic characterization of rat brain acetylcholinesterase modulated by lead and cartap: the ameliorative effect of Citrus limon fruit juice
  7. Attenuation of potassium dichromate and sodium arsenite toxicities by methanol extract of Rauvolfia vomitoria in mice
  8. Chronic exposure of industrial grade calcium carbide and ethylene glycol alter histological architecture of systemic organs by disrupting redox balance in rat
  9. Biochemical and histopathological effects of low dose vanadium in the healing of acetic acid-induced colitis in male wistar rats
  10. Glycine and L-Arginine supplementation ameliorates gastro-duodenal toxicity in a rat model of NSAID (Diclofenac)-gastroenteropathy via inhibition of oxidative stress
  11. Cocos nucifera L. oil alleviates lead acetate-induced reproductive toxicity in sexually-matured male Wistar rats
  12. Intracellular signaling molecules of nerve tissue progenitors as pharmacological targets for treatment of ethanol-induced neurodegeneration
  13. Glatiramer acetate treatment inhibits inflammatory responses and improves survival in a mice model of cecal ligation and puncture-induced sepsis
  14. Ciprofloxacin-induced cutaneous adverse drug events: a systematic review of descriptive studies
  15. Aqueous extract of Adansonia digitata prevents Cadmium Chloride-induced testicular damage in Wistar Rats
  16. Exposure to prolonged unpredictable light impairs spatial memory via induction of oxidative stress and tumor necrosis factor-alpha in rats
  17. Tamarindus indica. Linn leaves ameliorates experimental induced heart failure in Wistar rats
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