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Beneficial effect of Bidens pilosa L. (Asteraceae) in a rat model of colitis

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Published/Copyright: June 29, 2020
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 31 Issue 6

Abstract

Background

Bidens pilosa (BP) possessed anti-inflammatory, antioxidant, and immunomodulatory activities. Its beneficial effects on intestinal inflammation and oxidative stress in 2,4,6 trinitrobenzene sulfonic acid (TNBS) induced colitis in Wistar rats was evaluated.

Methods

Thirty female Wistar rats weighing 180–200 g were distributed into six groups (n = 5): non-colitic, untreated colitic and colitic rats treated graded doses of methanol extract of BP (50–400 mg/kg). Colitis was induced in rats by intracolonic instillation of 0.2 mL of 40 mg/mL TNBS. BP was administered two days pre-colitis induction and treatments continued until seven days post-colitis induction. A day after the last treatment, rats were euthanized, colon removed aseptically and response to treatment assessed. Phytochemical composition of BP was determined using the GC-MS.

Results

BP significantly reduced macroscopic colonic damage score, weight/length ratio, colonic lipid peroxidation level, leukocytes infiltration, and TNF-α level in comparison to untreated colitic rats (p ≤ 0.008). Similarly, treatment with 200 and 400 mg/kg BP prevented depletion of colonic glutathione level than other treatment groups (p ≤ 0.0002). Histological findings revealed that treatment with 400 mg/kg BP significantly preserved the mucosal epithelial layer. It also prevented ulceration and sloughing of the mucosal layers and reduced infiltration of inflammatory cells compared to other treatment groups. Among the 16 compounds identified were oleic acid (6.2%) and n-hexadecanoic acid (2.0%) with antioxidant anti-inflammatory activities.

Conclusions

The beneficial effects of BP in rat colitis might be related to the reduction of leucocytes infiltration, inhibition of oxidative stress and pro-inflammatory cytokines.

Keywords: antioxidant; B. pilosa; colitis; inflammation
Corresponding author: Oyindamola O. Abiodun, Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, PMB 200284, Ibadan, Oyo State, Nigeria, Tel.: +234703/096 4774, E-mail:

Acknowledgments

The technical assistance of Mrs. Sakirat Braimah of in vivo animal section of Malaria Research Laboratory, University of Ibadan during the course of the study is acknowledged.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: Experimental procedures and protocols used in this study conform to the “Guide to the care and use of animals in research and teaching” (NIH publications number 85–93 revised in 1985) and the University of Ibadan Animal Care and Use Research Ethics Committee (UI-ACCUREC/19/0015).

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Received: 2019-06-20
Accepted: 2020-02-04
Published Online: 2020-06-29

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jbcpp-2019-0166
Keywords for this article
antioxidant; B. pilosa; colitis; inflammation

Articles in the same Issue

  1. Reviews
  2. An evidence-based systematic review on emerging therapeutic and preventive strategies to treat novel coronavirus (SARS-CoV-2) during an outbreak scenario
  3. Testis and blood-testis barrier in Covid-19 infestation: role of angiotensin-converting enzyme 2 in male infertility
  4. An overview of high-mobility group box 1, a potent pro-inflammatory cytokine in asthma
  5. Original Articles
  6. The effect of novel simple saffron syrup on fatigue reduction in patients with multiple sclerosis
  7. Effects of atenolol injected into the nucleus accumbens septi in rats in the elevated plus-maze test
  8. Beneficial role of central anticholinergic agent in preventing the development of symptoms in mouse model of post-traumatic stress disorder
  9. Pulmonary function severity in relation to interleukin-23 levels in patients with psoriasis vulgaris
  10. Effect of dexamethasone on the expression of MMPs, adenosine A1 receptors and NFKB by human trabecular meshwork cells
  11. Beneficial effect of Bidens pilosa L. (Asteraceae) in a rat model of colitis
  12. Case Reports
  13. 4th A in a triple A syndrome – A rare case report
  14. Is COVID-19 spreading and curing silently: an observation of three family clusters in Bangladesh
  15. Letter to the Editor
  16. SARS-CoV-2 infection, oxidative stress and male reproductive hormones: can testicular-adrenal crosstalk be ruled-out?
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