Abstract
Background
In Nigerian traditional medicine, Rauwolfia vomitoria has been reported to be useful in the management of various human diseases, but there is no relevant information to substantiate its involvement in managing diseases arising from vascular dysfunction and oxidative stress. However, this study sought to investigate the antioxidant property of R. vomitoria and its effect on phophodiesterase-5 activity in vitro.
Methods
The antioxidant property was assessed through ferric-reducing antioxidant power (FRAP), copper chelation, and ABTS radical-scavenging activity. In addition, the effect of R. vomitoria on phosphodiesterase-5 (PDE-5) activity was assessed in vitro. Furthermore, analysis of phenolic compounds present in R. vomitoria was carried out using high-performance liquid chromatography (HPLC).
Results
The findings in this study revealed that R. vomitoria inhibited PDE-5 in a dose-dependent manner (IC50 = 252.42 μg/mL). Furthermore, the antioxidant activity of R. vomitoria was established through FRAP (19.68 mg AAE/g), ABTS radical-scavenging ability (74.25 mmol TEAC/g), and Cu2+-chelating ability (IC50 = 0.13 mg/mL).
Conclusions
The antioxidant property of R. vomitoria and its inhibitory effect on PDE-5 could be useful in the management of diseases arising from vascular dysfunction and oxidative stress.
Author contributions: All authors have accepted responsibility for the entire content of this submitted manuscript and approved its submission.
Research funding: None declared.
Competing interests: Authors state no conflict of interest.
References
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- HPLC phenolic fingerprinting, antioxidant and anti-phosphodiesterase-5 properties of Rauwolfia vomitoria extract
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Articles in the same Issue
- Review
- Cardioprotective mechanism of FTY720 in ischemia reperfusion injury
- Original Articles
- Effect of Gmelina arborea on learning and memory in amnesia-induced and non-amnesia groups of albino Wistar rats
- The acute effects of decaffeinated versus caffeinated coffee on reaction time, mood and skeletal muscle strength
- Prevention of short-term memory impairment by Bryophyllum pinnatum (Lam.) Oken and its effect on acetylcholinesterase changes in CCl4-induced neurotoxicity in rats
- Modulatory effects of artichoke (Cynara scolymus L.) leaf extract against oxidative stress and hepatic TNF-α gene expression in acute diazinon-induced liver injury in rats
- Evaluation of biomarkers of stress in chronic stress-exposed comorbid depression model Wistar rats
- Drospirenone-containing oral contraceptives do not affect glucose regulation and circulating corticosterone
- Vitamin C, omega-3 and paracetamol pharmacokinetic interactions using saliva specimens as determiners
- Comparison of ocular pharmacokinetics of etoposide and its nanoemulsion after subtenon administration in rabbits
- Exploring the therapeutic potential of sodium benzoate in acetic acid-induced ulcerative colitis in rats
- Short Communication
- HPLC phenolic fingerprinting, antioxidant and anti-phosphodiesterase-5 properties of Rauwolfia vomitoria extract
- Miscellaneous
- Clopidogrel-induced hemarthrosis following knee replacement