Sub-chronic indomethacin treatment and its effect on the male reproductive system of albino rats: possible protective role of black tea extract
-
Ishwar B. Bagoji
Abstract
Background:
Indomethacin is commonly used as a nonsteroidal anti-inflammatory drug (NSAID) to treat inflammation, arthritis and joint pains. Unfortunately, it has a wide range of adverse effects on the physiological system, including gonads. This study aimed to assess possible beneficial effects of black tea extract (BTE) against indomethacin-induced alteration of gonadal hormone levels in male rats.
Methods:
Adult male rats were divided into Group I (control), Group II (indomethacin, 5 mg/kg body weight [bwt.]; i.p., 21 days), Group III (BTE, 2.5 g tea leaf/dL of water, i.e. 2.5% of aqueous BTE, orally, 21 days) and Group IV (indomethacin+BTE, 21 days). Sperm count and motility, serum luteinising hormone (LH), follicle-stimulating hormone (FSH) and testosterone, along with histopathology of testes were studied. One-way ANOVA, followed by post-hoc t-test were conducted.
Results:
Indomethacin-treated rats showed significant decrease in testicular weight, sperm count, sperm motility, serum gonadotropins and testosterone concentrations. Histopathology of the testes showed tortuous and distorted seminiferous tubules, marked thickening of the tubular basement membrane, reduced spermatogenesis process (>30%) and marked decrease in the number of interstitial cells of Leydig in indomethacin-treated rats. Interestingly, rats supplemented with BTE showed remarkable improvements in testicular weight gain, sperm count and motility, serum gonadotropins and testosterone concentrations, along with testicular histopathology.
Conclusions:
The results suggest that BTE might have potential ameliorative effects against sub-chronic indomethacin-induced alteration of gonadal hormone levels in male albino rats.
Acknowledgments
The authors greatly acknowledge BLDE University Vijayapura, Karnataka, India, for providing financial assistance (Ref. No. BLDEU/REG/R&D/2014-15/1121) to carry out this work.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved its submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organisation(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Crofford LJ. Use of NSAIDs in treating patients with arthritis. Arthritis Res Ther 2013;15:S2.10.1186/ar4174Search in Google Scholar PubMed PubMed Central
2. Albert O, Desdoits-Lethimonier C, Lesné L, Legrand A, Guillé F, Bensalah K. Paracetamol, aspirin and indomethacin display endocrine disrupting properties in the adult human testis in vitro. Hum Reprod 2013;28:1890–8.10.1093/humrep/det112Search in Google Scholar PubMed
3. Kelly RW. Prostaglandin synthesis in the male and female reproductive tract. J Reprod Fert 1981;62:293–304.10.1530/jrf.0.0620293Search in Google Scholar PubMed
4. Saeed SA, Anwar N, Khan KM, Sarfraz N. Effect of chronic treatment with a cyclooxygenase inhibitor on reproductive parameters in male rat. J Ayub Med Coll Abbottabad 2009; 21:66–71.Search in Google Scholar
5. Das KK, Gupta AD, Dhundasi SA, Patil AM, Das SN, Ambekar JG. Protective role ofL-ascorbic acid on antioxidant defense system in erythrocytes of albino rats exposed to nickel sulphate. Biometals 2007;20:177–84.10.1007/s10534-006-9025-zSearch in Google Scholar PubMed
6. Das KK, Dasgupta S. Effect of nickel sulfate on testicular steroidogenesis in rats during protein restriction. Environ Health Perspect 2002;110:923–6.10.1289/ehp.02110923Search in Google Scholar PubMed PubMed Central
7. Bagoji IB, Doshi MA, Yendigeri SM, Patil BG, Patil BB, Das KK. Black tea extract ameliorates indomethacin induced changes in testicular histopathology of albino rats. J Young Pharma 2014;6:53–7.10.5530/jyp.2014.3.9Search in Google Scholar
8. Kamath SM, Stoecker BJ, Davis-Whitenack ML, Smith MM, Adeleye BO, Sangiah S. Absorption, retention and urinary excretion of chromium-51 in rats pretreated with indomethacin and dosed with dimethylprostaglandin E2, misoprostol or prostacyclin. J Nutr 1997;127:478–82.10.1093/jn/127.3.478Search in Google Scholar PubMed
9. Bagoji IB, Jargar JG, Yendigeri SM, Doshi MA, Patil BG, Das KK. Effect of supplementation of black tea extract on indomethacin induced alteration of pathophysiology of rat liver. J Chem Pharmaceut Res 2015;7:597–604.Search in Google Scholar
10. Venkataraman BV, Joseph T, Shetty PS, Stephen PM. Acetylcholine levels of rat brain and heart in starvation and protein restriction. Indian J Physiol Pharmacol 1984;28:223–8.Search in Google Scholar
11. Knobil E. The neuroendocrine control of the menstrual cycle. Rec Prog Horm Res 1980;36:52–88.10.1016/B978-0-12-571136-4.50008-5Search in Google Scholar PubMed
12. Marshall JC. Clinics in endocrinology and metabolism. Investigative procedures. Clinic Endocrinol. Metab 1975;4:545–67.10.1016/S0300-595X(75)80047-8Search in Google Scholar
13. Tijssen P. Practice & theory of enzyme immunoassays. Amsterdam: Elservier, 1985.Search in Google Scholar
14. Sanghishetti V, Ghongane BB, Nayak BB. Effect of vitamin C on male fertility in rats subjected to forced swimming stress. J Clin Diag Res 2014;8:HC05–HC08.Search in Google Scholar
15. Culling CF, Ellison RT, Barr WT. Cellular pathology techniques, 4th ed. London: Butterworth and Co, 1985.10.1016/B978-0-407-72903-2.50031-9Search in Google Scholar
16. Khan RA. Protective effects of Launaea procumbens on rat testis damage by CCl4. Lipids Health Disease 2012;11:103.10.1186/1476-511X-11-103Search in Google Scholar PubMed PubMed Central
17. Jargar JG, Yendigeri SM, Dhundasi SA, Das KK. Protective effect of vitamin E (αtocopherol) on nickel induced alteration of testicular pathophysiology in alloxan treated diabetic rats. Int J Clin Expt Physiol 2014;1:290–7.10.4103/2348-8093.149762Search in Google Scholar
18. Joshi SC, Mathur R, Gulati N. Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat. Toxicol Ind Health 2007;23:439–44.10.1177/0748233707080908Search in Google Scholar PubMed
19. Salama AF, El-Bahr SM. Effect of Curcumin on cadmium-induced oxidative testicular damage in rats. J Med Res Inst 2007;28:167–73.Search in Google Scholar
20. Silveira LF, Latronico AC. Approach to the patient with hypogonadotropic hypogonadism.J Clin Endocrinol Metab 2013;98:1781–8.10.1210/jc.2012-3550Search in Google Scholar PubMed
21. Shaha C, Tripathi R, Mishra DP. Male germ cell apoptosis: regulation and biology. Philos Trans R Soc Lond B Biol Sci 2010;365:1501–15.10.1098/rstb.2009.0124Search in Google Scholar PubMed PubMed Central
22. Elbetieha A, Daas SI, Khamas W, Darmani H. Evaluation of the toxic potentials of cypermethrin pesticide on some reproductive and fertility parameters in the male rats.Arch Environ Contam Toxicol 2001;41:522–8.10.1007/s002440010280Search in Google Scholar PubMed
23. Sofikitis N, Giotitsas N, Tsounapi P, Baltogiannis D, Giannakis D, Pardalidis N. Hormonal regulation of spermatogenesis and spermiogenesis. J Steroid Biochem Mol Biol 2008;109:323–30.10.1016/j.jsbmb.2008.03.004Search in Google Scholar PubMed
24. Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes Oxid Med Cell Longev 2008;1:15–24.10.1007/978-0-387-09597-4_9Search in Google Scholar PubMed
25. Lodhi GM, Hameed W, Tausif A, Latif R, Aslam M. Effects of ascorbic acid and alpha tocopherol supplementation on basal testosterone cortisol ratio in male sprague dawley rats. Pak J Physiol 2011;7:7–10.Search in Google Scholar
26. Omu AE, Al-Azemi MK, Al-Maghrebi M, Mathew CT, Omu FE, Kehinde EO, et al. Molecular basis for the effects of zinc deficiency on spermatogenesis: an experimental study in the Sprague-Dawley rat model. Indian J Urol 2015;31:57–64.10.4103/0970-1591.139570Search in Google Scholar PubMed PubMed Central
27. Jargar JG, Yendigeri SM, Hattiwale SH, Dhundasi SA, Das KK. α -Tocopherol ameliorates nickel induced testicular oxidative and nitrosative stress in albino rats. J Basic Clin Physiol Pharmacol 2012;23:77–82.10.1515/jbcpp-2012-0013Search in Google Scholar PubMed
28. Naughton CK, Nangia AK, Agarwal A. Pathophysiology of varicoceles in male infertility. Hum Reprod Update 2001;7:473–81.10.1093/humupd/7.5.473Search in Google Scholar PubMed
29. Chandra AK, Chatterjee A, Ghosh R, Sarkar M. Vitamin E-supplementation protects chromium (VI)-induced spermatogenic and steroidogenic disorders in testicular tissues of rats. Food Chem Toxicol 2010;48:972–9.10.1016/j.fct.2010.01.008Search in Google Scholar PubMed
30. Sha’bani N, Miraj S, Kohpayei MR, Namjoo AR. Survey of the detoxification effect of green tea extract on the reproductive system in rats exposed to lead acetate. Adv Biomed Res 2015;4:155.10.4103/2277-9175.161582Search in Google Scholar PubMed PubMed Central
©2017 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Isolated heart models for studying cardiac electrophysiology: a historical perspective and recent advances
- Reproduction
- Sub-chronic indomethacin treatment and its effect on the male reproductive system of albino rats: possible protective role of black tea extract
- The effect of extracellular ATP on rat uterine contraction from different gestational stages and its possible mechanisms of action
- Cardiovascular Function
- Effect of cardiovascular and muscular endurance is not associated with stress fracture incidence in female military recruits: a 12-month follow up study
- Cardiovascular-Pulmonary Interactions
- Comparison of structured and unstructured physical activity training on predicted VO2max and heart rate variability in adolescents – a randomized control trial
- Oxidative Stress
- Aminoguanidine pretreatment prevents methotrexate-induced small intestinal injury in the rat by attenuating nitrosative stress and restoring the activities of vital mitochondrial enzymes
- Anti-aging effects of M2000 (β-D-mannuronic acid) as a novel immunosuppressive drug on the enzymatic and non-enzymatic oxidative stress parameters in an experimental model
- Metabolism
- Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models
- Effect of adrenocorticotropic hormone on UCP1 gene expression in brown adipocytes
- Phytotherapy
- Antidiabetic effect of Ruta montana L. in streptozotocin-induced diabetic rats
- Ethnopharmacological investigation of the aerial part of Phragmites karka (Poaceae)
Articles in the same Issue
- Frontmatter
- Review
- Isolated heart models for studying cardiac electrophysiology: a historical perspective and recent advances
- Reproduction
- Sub-chronic indomethacin treatment and its effect on the male reproductive system of albino rats: possible protective role of black tea extract
- The effect of extracellular ATP on rat uterine contraction from different gestational stages and its possible mechanisms of action
- Cardiovascular Function
- Effect of cardiovascular and muscular endurance is not associated with stress fracture incidence in female military recruits: a 12-month follow up study
- Cardiovascular-Pulmonary Interactions
- Comparison of structured and unstructured physical activity training on predicted VO2max and heart rate variability in adolescents – a randomized control trial
- Oxidative Stress
- Aminoguanidine pretreatment prevents methotrexate-induced small intestinal injury in the rat by attenuating nitrosative stress and restoring the activities of vital mitochondrial enzymes
- Anti-aging effects of M2000 (β-D-mannuronic acid) as a novel immunosuppressive drug on the enzymatic and non-enzymatic oxidative stress parameters in an experimental model
- Metabolism
- Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models
- Effect of adrenocorticotropic hormone on UCP1 gene expression in brown adipocytes
- Phytotherapy
- Antidiabetic effect of Ruta montana L. in streptozotocin-induced diabetic rats
- Ethnopharmacological investigation of the aerial part of Phragmites karka (Poaceae)
