Investigation of antinociceptive activity of methanolic extract of Persicaria orientalis leaves in rodents

Prawej Ansari; Md. Josim Uddin; Shafia Akther; Shofiul Azam; Md Kayes Mahmud; Sohel Bin Azad; Ahsan Ullah; J.M.A. Hannan

doi:10.1515/jbcpp-2016-0018

Article

Investigation of antinociceptive activity of methanolic extract of Persicaria orientalis leaves in rodents

Prawej Ansari , Md. Josim Uddin , Shafia Akther , Shofiul Azam , Md Kayes Mahmud , Sohel Bin Azad , Ahsan Ullah and J.M.A. Hannan

Published/Copyright: January 25, 2017

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From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 28 Issue 2

Abstract

Background:

This study aims to evaluate the scientific basis of traditional application of Persicaria orientalis for reducing pain and inflammation.

Methods:

An in vitro method was performed to investigate the presence of the anti-inflammatory activity of methanolic crude extract of P. orientalis. In addition, an in vivo study was conducted in which the hot-plate and tail immersion methods were applied to explore the acute effect of P. orientalis on analgesia. The potency to inhibit chronic inflammation in mice was justified by the carrageenan-induced paw edema and formalin-induced edema methods. For all in vivo testing in animal models (albino mice and rats), plant extract was given via the oral route at doses of 250 mg/kg and 500 mg/kg.

Results:

The methanolic extract of P. orientalis produced a significant (p<0.001) inhibition of analgesia with a prolongation of pain response time by 61.80% at 500 mg/kg. The extract also exhibited a potential anti-inflammatory (56.99%) effect, which was also statistically significant (p<0.001). The present study suggests that the methanolic extract of P. orientalis has potential anti-inflammatory as well as analgesic activity and this extract is effective in the treatment of both acute and chronic pain.

Conclusions:

Our current study revealed pharmacological properties of the methanolic extract of P. orientalis and also gave a solid scientific platform against its traditional use. The protecting ability of P. orientalis against inflammatory stimuli may be due to phenolic or flavonoid compounds which we have found through phytochemical analysis.

Keywords: carrageenan-induced paw edema; Eddy’s hot plate; formalin-induced edema; inflammation

Acknowledgments

All authors are very thankful to the International Islamic University Chittagong and North South University, Dhaka, Bangladesh for their arrangement of chemicals and animal models required in this experimental study.

Author contributions: All of the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. Prawej Ansari, Md. Josim Uddin and JMA Hannan designed the current project, performed the experiments, and are responsible for data interpretation and statistical analysis, Prawej Ansari and Shofiul Azam collected the experimental chemicals and wrote the manuscript, Sohel Bin Azad and Md Kayes Mahmud helped in experiments by collecting plants, and Shofiul Azam and Ahsan Ullah helped in data collection and plant identification. Finally, Prawej Ansari edited the manuscript. All authors read and approved the final version of the manuscript.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design, in the collection, analysis, and interpretation of data, in the writing of the report, or in the decision to submit the report for publication.

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Received: 2016-2-18

Accepted: 2016-10-12

Published Online: 2017-1-25

Published in Print: 2017-3-1

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Articles in the same Issue

https://doi.org/10.1515/jbcpp-2016-0018

Keywords for this article

carrageenan-induced paw edema; Eddy’s hot plate; formalin-induced edema; inflammation