Antidiarrheal and antinociceptive activities of ethanol extract and its chloroform and pet ether fraction of Phrynium imbricatum (Roxb.) leaves in mice
-
Mohammed Munawar Hossain
,
S.M. Zahid Hosen
Abstract
Background:
The objective of the study was to evaluate the antidiarrheal and antinociceptive activities of ethanol extract and its chloroform and pet ether fraction of Phrynium imbricatum (Roxb.) leaves in mice.
Methods:
In the present study, the dried leaves of P. imbricatum were subjected to extraction with ethanol, and then it was fractioned by chloroform and pet ether solvent. Antidiarrheal effects were tested by using castor oil-induced diarrhea, castor oil-induced enteropooling, and gastrointestinal transit test. Antinociceptive activity was evaluated by using the acetic acid-induced writhing test and formalin-induced paw licking test.
Results:
The standard drug loperamide (5 mg/kg) showed significant (p<0.001) inhibitory activity against castor oil-induced diarrhea, in which all the examined treatments decreased the frequency of defecation and were found to possess an anti-castor oil-induced enteropooling effect in mice by reducing both weight and volume of intestinal content significantly, and reducing the propulsive movement in castor oil-induced gastrointestinal transit using charcoal meal in mice. The results showed that the ethanol extract of P. imbricatum leaves has significant dose-dependent antinociceptive activity, and among its two different fractions, the pet ether fraction significantly inhibited the abdominal writhing induced by acetic acid and the licking times in formalin test at both phases.
Conclusions:
These findings suggest that the plant may be a potential source for the development of a new antinociceptive drug and slightly suitable for diarrhea, as it exhibited lower activity. Our observations resemble previously published data on P. imbricatum leaves.
Acknowledgments
The authors are grateful to the authority of the International Islamic University Chittagong, Bangladesh, for providing the facilities to conduct this research work. The authors thank the GUSTO A Research Group for financial support. The authors are also thankful to all members of the GUSTO A Research Group, especially to Mr. Shabbir Ahmed, Department of Pharmacy, IIUC; Mr. Md. Akramul Hoque, Department of Pharmacy, IIUC; and Mr. Nishan Chakrabarty, Department of Pharmacy, IIUC, Bangladesh, for their kind help in the experiments.
Author contributions: MMH and MSHK collected the plant leaves and prepared the extract and fractions. MMH and MSHK also carried out the study design, performed the experiments, collected and interpreted the data, prepared the manuscript, and performed statistical analysis. MSHK wrote the first draft of the manuscript. MAMD, MSIA, SMZH, RD, and MMNU helped in the experiments, data collection, and literature search. The intermodel relationship idea was uniquely given by MSHK. MMR supervised the study design and data interpretation. All the authors have accepted responsibility for the entire content of the submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2017 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Natural product for the treatment of Alzheimer’s disease
- Behavior and Neuroprotection
- Evidence for the involvement of the GABA-ergic pathway in the anticonvulsant activity of the roots bark aqueous extract of Anthocleista djalonensis A. Chev. (Loganiaceae)
- Cardiovascular Function
- Cutaneous temperature sensitivity alteration in subjects with chronic stroke sequelae – pharmacological perspectives
- Combinatorial therapy of exercise-preconditioning and nanocurcumin formulation supplementation improves cardiac adaptation under hypobaric hypoxia
- Oxidative Stress
- Phellinus rimosus improves mitochondrial energy status and attenuates nephrotoxicity in diabetic rats
- Hepatoprotective effects of Vaccinium arctostaphylos against CCl4-induced acute liver injury in rats
- The impact of vitamin C on the relationship among inflammation, lipid peroxidation and platelet activation during analgesic nephropathy in rats
- Phytotherapy
- Antidiarrheal and antinociceptive activities of ethanol extract and its chloroform and pet ether fraction of Phrynium imbricatum (Roxb.) leaves in mice
- Amelioration of hyperglycemia and associated metabolic abnormalities by a combination of fenugreek (Trigonella foenum-graecum) seeds and onion (Allium cepa) in experimental diabetes
- An ethanolic extract of Desmodium adscendens exhibits antipsychotic-like activity in mice
Articles in the same Issue
- Frontmatter
- Review
- Natural product for the treatment of Alzheimer’s disease
- Behavior and Neuroprotection
- Evidence for the involvement of the GABA-ergic pathway in the anticonvulsant activity of the roots bark aqueous extract of Anthocleista djalonensis A. Chev. (Loganiaceae)
- Cardiovascular Function
- Cutaneous temperature sensitivity alteration in subjects with chronic stroke sequelae – pharmacological perspectives
- Combinatorial therapy of exercise-preconditioning and nanocurcumin formulation supplementation improves cardiac adaptation under hypobaric hypoxia
- Oxidative Stress
- Phellinus rimosus improves mitochondrial energy status and attenuates nephrotoxicity in diabetic rats
- Hepatoprotective effects of Vaccinium arctostaphylos against CCl4-induced acute liver injury in rats
- The impact of vitamin C on the relationship among inflammation, lipid peroxidation and platelet activation during analgesic nephropathy in rats
- Phytotherapy
- Antidiarrheal and antinociceptive activities of ethanol extract and its chloroform and pet ether fraction of Phrynium imbricatum (Roxb.) leaves in mice
- Amelioration of hyperglycemia and associated metabolic abnormalities by a combination of fenugreek (Trigonella foenum-graecum) seeds and onion (Allium cepa) in experimental diabetes
- An ethanolic extract of Desmodium adscendens exhibits antipsychotic-like activity in mice
