Anti-diabetic effects of aqueous prickly lettuce (Lactuca scariola Linn.) leaves extract in alloxan-induced male diabetic rats treated with nickel (II)
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Kailash S. Chadchan
Abstract
Background: Hattaraki pallye or prickly lettuce (Lactuca scariola Linn.) is one among several green leafy plants that grow in north Karnataka; it is usually consumed by the people of this region and is found to be antidiabetic in nature. The objective of this study is to evaluate hypoglycemic activities of supplementation with aqueous extract of prickly lettuce (L. scariola) leaves in vivo in acute and subchronic exposure with or without nickel (II) along with its glucose reduction capabilities with or without nickel (II) at pH 7.0 and 9.0 in vitro.
Methods: Percentage glucose reduction (in vitro) was determined by glucose oxidase-peroxidase enzymatic method at pH 7.0 and pH 9.0 using UV-Vis spectrophotometer. Hypoglycemic activities of L. scariola were carried out in alloxan-induced male diabetic rats at both acute and subchronic exposure.
Results: The results showed a significant alteration in the λmax value of Ni (II) in combination with L. scariola leaves extracts at both pH 7.0 and 9.0. The aqueous extract also produced a significant reduction in the glucose concentration at pH 7.0 and pH 9.0 even in presence of Ni (II) in vitro. Lactuca scariola leaves in either acute or subchronic supplementation showed a greater glucose tolerance and hypoglycemic regulation of blood sugar in diabetic rats with or without nickel (II) treatments.
Conclusions:Lactuca scariola leaves can be a substitute for synthetic drugs to treat diabetic patients.
Acknowledgments
Authors deeply acknowledge the constant guidance of Prof. Kusal K. Das of BLDE University, Bijapur, India, in carrying out the entire work. Technical help from Dr. C.C. Simpi, Dr. R.V. Kulkarni and Shri. Managond of BLDEA’s College of Pharmacy, Bijapur, Karnataka, are acknowledged. Prof. smt. L.M. Malaghan, Dr. M.B. Mulimani and Dr. Paramanna D. of B.L.D.EA’s S.B. Arts and K.C.P. Science College, Bijapur, are also acknowledged for identifying the plant.
Conflict of interest: The authors stated that there are no conflicts of interest regarding the publication of this article.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: Authors received research funding from B.L.D.E.A’s V.P. Dr. P.G. Halakatti College of Engineering and Technology, Bijapur, to carry out this project.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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- Anti-hyperalgesic and anti-nociceptive potentials of standardized grape seed proanthocyanidin extract against CCI-induced neuropathic pain in rats
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- Protective effect of dietary fenugreek (Trigonella foenum-graecum) seeds and garlic (Allium sativum) on induced oxidation of low-density lipoprotein in rats
- Metabolism
- Anti-diabetic effects of aqueous prickly lettuce (Lactuca scariola Linn.) leaves extract in alloxan-induced male diabetic rats treated with nickel (II)
- Sildenafil, a phosphodiesterase type 5 inhibitor, attenuates diabetic nephropathy in STZ-induced diabetic rats
- Inflammation
- Evaluation of analgesic, anti-inflammatory, thrombolytic and hepatoprotective activities of roots of Premna esculenta (Roxb)
- Evaluation of anti-inflammatory potential of the ethanolic extract of the Saussurea lappa root (costus) on adjuvant-induced monoarthritis in rats
- Miscellaneous
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Articles in the same Issue
- Frontmatter
- Behavior and Neuroprotection
- Neuroprotective effect of hydroxy safflor yellow A against cerebral ischemia-reperfusion injury in rats: putative role of mPTP
- Anti-hyperalgesic and anti-nociceptive potentials of standardized grape seed proanthocyanidin extract against CCI-induced neuropathic pain in rats
- Protective effects of forced exercise against nicotine-induced anxiety, depression and cognition impairment in rat
- Oxidative Stress
- Effects of long-term administration of aspartame on biochemical indices, lipid profile and redox status of cellular system of male rats
- Protective effect of dietary fenugreek (Trigonella foenum-graecum) seeds and garlic (Allium sativum) on induced oxidation of low-density lipoprotein in rats
- Metabolism
- Anti-diabetic effects of aqueous prickly lettuce (Lactuca scariola Linn.) leaves extract in alloxan-induced male diabetic rats treated with nickel (II)
- Sildenafil, a phosphodiesterase type 5 inhibitor, attenuates diabetic nephropathy in STZ-induced diabetic rats
- Inflammation
- Evaluation of analgesic, anti-inflammatory, thrombolytic and hepatoprotective activities of roots of Premna esculenta (Roxb)
- Evaluation of anti-inflammatory potential of the ethanolic extract of the Saussurea lappa root (costus) on adjuvant-induced monoarthritis in rats
- Miscellaneous
- Pathophysiological alterations induced by sustained 35-GHz radio-frequency energy heating
