Hepatoprotective and antioxidant activity of hydromethanolic extract of Daniella oliveri leaves in carbon tetrachloride-induced hepatotoxicity in rats

Samuel Okwudili Onoja; Gideon Kelechi Madubuike; Maxwell Ikechukwu Ezeja

doi:10.1515/jbcpp-2014-0087

Article

Hepatoprotective and antioxidant activity of hydromethanolic extract of Daniella oliveri leaves in carbon tetrachloride-induced hepatotoxicity in rats

Samuel Okwudili Onoja , Gideon Kelechi Madubuike and Maxwell Ikechukwu Ezeja

Published/Copyright: April 28, 2015

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From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 26 Issue 5

Abstract

Background:Daniella oliveri is a deciduous plant that is commonly found in savanna and open grassland. Various parts of the plant is used by herbalist in the management of different ailments. The present study aims at investigating the hepatoprotective and antioxidant activity of the methanolic extract of D. oliveri leaves.

Methods: The hepatoprotective activity was investigated using carbon tetrachloride (CCl₄)-induced hepatotoxicity in albino rats. The antioxidant activity was determined using both in vitro (2, 2-diphenyl-1-picrylhydrazine photometric assay) and in vivo (malondialdehyde and catalase level assay) models.

Results: The pretreatment with extract (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) produced a significant (p<0.05) dose-dependent increase in hepatoprotective activity when compared with the negative control group. The extract (25–400 μg/mL concentration) produced a concentration-dependent increase in antioxidant activity in 2, 2-diphenyl-1-picrylhydrazine (DPPH) photometric assay. The IC₅₀ of the extract in DPPH photometric assay was 400 μg/mL concentrations. The extract and silymarin showed a significant (p<0.05) dose-dependent increase in catalase level in treated rats when compared with the negative control group. Also, the extract and silymarin produced a significant (p<0.05) dose-dependent decrease in malondialdehyde level in treated rats when compared with the negative control group.

Conclusions: The results of the study suggest that D. oliveri leaves has a potent hepatoprotective activity that may be linked to its antioxidant activities and validates its use in the traditional management of liver disorders.

Keywords: carbon tetrachloride; catalase; Daniella oliveri; hepatoprotective activity; malondialdehyde; silymarin

Corresponding author: Samuel Okwudili Onoja, Department of Veterinary Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, Umudike, P.M.B 7267 Umuahia, Abia State, Nigeria, Phone: +234-803-061-3032, E-mail: samonreal@yahoo.com

Acknowledgments

The authors are grateful to Mr. A. O. Ozioko for the identification of the plant material.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2014-8-3

Accepted: 2015-3-25

Published Online: 2015-4-28

Published in Print: 2015-9-1

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Articles in the same Issue

https://doi.org/10.1515/jbcpp-2014-0087

Keywords for this article

carbon tetrachloride; catalase; Daniella oliveri; hepatoprotective activity; malondialdehyde; silymarin