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Improving delivery capacity of soy 7S protein via high pressure homogenization: in the case of vitamin D3

  • Zhengsong Chen , Wenjing Zhong , Jie Xia and Jianhua Zhu EMAIL logo
Published/Copyright: February 17, 2025

Abstract

In this work, high pressure homogenization treated soy 7S protein (HPH–7S) was employed as carrier to load and delivery VD3. The encapsulation efficiency (EE) (97.88 %) and bio-accessibility (85.69 %) of VD3 was markedly improved by HPH-7S as compared with that of untreated 7S protein (92.63 %, 73.36 %), while the particle size (80.1 nm) was prominently smaller than that encapsulated by untreated 7S protein (243.3 nm). The photochemical and thermal stability of VD3 in VD3@HPH-7S nanoparticles (VD3@HPH-7S-NPs) was superior to that of VD3 protected by 7S protein. The enhancement of delivery capacity of HPH-7S may be due to the change in protein structure. i.e. the peptide chains of the proteins unfolded during homogenization treatment, exposing more hydrophobic groups and rearranging the tertiary structure, which was verified by Fourier transform infrared, endogenous fluorescence spectrum and surface hydrophobicity.


Corresponding author: Jianhua Zhu, College of Food Science and Technology, Shaoguan University, Shaoguan 512005, China, E-mail:

Award Identifier / Grant number: 2022A1515010772

  1. Research ethics: Not applicable.

  2. Informed consent: Not applicable.

  3. Author contributions: Zhengsong Chen: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing-original draft, Writing-review & editing. Wenjing Zhong: Investigation, Methodology, Validation, Writing-review & editing. Jie Xia: Investigation, Methodology, Validation, Writing-review & editing. Jianhua Zhu: Investigation, Methodology, Validation, Writing-review & editing, Project administration, Funding acquisition.

  4. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  5. Conflict of interest: The authors state no conflict of interest.

  6. Research funding: This research was supported by grant from the Guangdong Basic and Applied Basic Research Foundation (No. 2022A1515010772).

  7. Data availability: The data that has been used is confidential.

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Received: 2024-11-13
Accepted: 2025-01-26
Published Online: 2025-02-17

© 2025 Walter de Gruyter GmbH, Berlin/Boston

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