Development of dcELISA Method for Rapid Detection of β-conglycinin in Soybean
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Shiyao Zhang
Abstract
In this assay, rabbit anti-β-conglycinin polyclonal antibody (Pab) was used as primary antibody and goat anti-rabbit IgG-horseradish peroxidase (HRP) was used as secondary antibody. The optimum incubating time of blocking, HRP and TMB (3, 3’5, 5’-tetramethylbenzidine) detected by the response surface and orthogonal test was 58, 140, and 20 min, respectively. The practical working range for the determination of β-conglycinin was 20–320 ng ml−1. The recoveries of β-conglycinin in spiked soybean samples were between 95.8 % and 101.2 % with the relative standard deviation less than 6.9 % (intra-assay) and 8.2 % (inter-assay). The current method was used to analyze 80 different soybean obtained from different region of Jilin province and the detected results using the direct ELISA. Compared with the competitive ELISA, The results showed contents had no difference between two methods. The dcELISA assay provides a specific and sensitive method for detecting of soybean β-conglycinin in actual production.
Funding statement: This study was supported by grant from Youth fund project of national natural science fund (31101719); “Twelfth five-year” national science and technology support projects (2013BAD17B0) in Jilin province the development of science and technology plan projects youth scientific research fund (201201098); Jilin province education department “twelfth five-year” science and technology research program (2015198).
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Research Articles
- Kinetic Modeling and Optimization of Milk Coagulation Affected by Several Prevalent Cheesemaking Factors and Essence Addition
- Juglone Thermosensitive Liposomes: Preparation, Characterization, in vitro Release and Hyperthermia Cell Evaluation
- Effects of Ultrasound on the Physicochemical Properties and Antioxidant Activities of Chestnut Polysaccharide
- Effect of Spray Drying Temperature and Agave Fructans Concentration as Carrier Agent on the Quality Properties of Blackberry Powder
- Development of dcELISA Method for Rapid Detection of β-conglycinin in Soybean
- Characteristics of Dynamics Sorption Isotherms of Date Flesh Powder Rich in Fiber
- Modelling Effective Moisture Diffusivity of Pumpkin (Cucurbita moschata) Slices under Convective Hot Air Drying Condition
- Quality Retention Enhancement in Canned Potato and Radish Using Reciprocating Agitation Thermal Processing
- Bacteriocin Produced from Lactobacillus plantarum ATM11: Kinetic and Thermodynamic Studies
- Optimization of Storage Conditions of Malta (Citrus sinensis) Using Response Surface Methodology
Articles in the same Issue
- Frontmatter
- Research Articles
- Kinetic Modeling and Optimization of Milk Coagulation Affected by Several Prevalent Cheesemaking Factors and Essence Addition
- Juglone Thermosensitive Liposomes: Preparation, Characterization, in vitro Release and Hyperthermia Cell Evaluation
- Effects of Ultrasound on the Physicochemical Properties and Antioxidant Activities of Chestnut Polysaccharide
- Effect of Spray Drying Temperature and Agave Fructans Concentration as Carrier Agent on the Quality Properties of Blackberry Powder
- Development of dcELISA Method for Rapid Detection of β-conglycinin in Soybean
- Characteristics of Dynamics Sorption Isotherms of Date Flesh Powder Rich in Fiber
- Modelling Effective Moisture Diffusivity of Pumpkin (Cucurbita moschata) Slices under Convective Hot Air Drying Condition
- Quality Retention Enhancement in Canned Potato and Radish Using Reciprocating Agitation Thermal Processing
- Bacteriocin Produced from Lactobacillus plantarum ATM11: Kinetic and Thermodynamic Studies
- Optimization of Storage Conditions of Malta (Citrus sinensis) Using Response Surface Methodology
