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Green ultrasound-assisted extraction and life cycle assessment of lutein from marigold flowers using biocompatible surfactants

  • Neha Maheshwari , Vishal V. Khanpit and Ashwin Kannan EMAIL logo
Published/Copyright: November 3, 2023
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International Journal of Chemical Reactor Engineering
From the journal International Journal of Chemical Reactor Engineering Volume 22 Issue 1

Abstract

The objective of this work is to extract lutein from marigold petals by the ultrasound assisted extraction employing surfactant based aqueous solution. In this study the effect of various parameters i.e. different biocompatible surfactants, temperature, extraction time, pulse mode (ON/OFF), ultrasonicator power, solid/liquid (S/L) ratio, surfactant concentration were investigated. Under the optimum conditions (temperature: 35 °C, extraction time: 20 min, pulse mode (ON/OFF): 20 s ON/OFF, surfactant concentration: 1 % (v/v), S/L: 7.5, ultrasonicator power: 370 W), the maximum lutein content of 12.18 ± 0.39 mg/g was obtained. Further, the comparative study was done for the conventional extraction (CE) and the ultrasound assisted extraction (UAE), both using surfactants aqueous solution as solvent for the extraction of lutein. The results revealed that the amount of lutein obtained using UAE method was notably higher than the CE method. Furthermore, CE and UAE methods were compared in terms of their environmental impact by life cycle assessment approach. Global warming potential (GWP) was found to be lower for UAE (0.91 kg CO2/kg of lutein extract) than CE (1.55 kg CO2/kg of lutein extract). An antioxidant activity of lutein extract was studied using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The results revealed that lutein can be extracted by UAE without affecting the antioxidant activity of lutein extract. The aforesaid results suggest that UAE employing surfactant based aqueous solution method is suitable for the extraction of lutein from marigold petals.

Keywords: lutein; biocompatible surfactants; ultrasound assisted extraction; antioxidant
Corresponding author: Ashwin Kannan, Department of Chemical Engineering, Visveswaraya National Institute of Technology, Nagpur 440010, India, E-mail:

  1. Research ethics: Not applicable.

  2. Author contributions: The authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: The authors state no conflict of interest.

  4. Research funding: This research received no external funding.

  5. Data availabilty: Not applicable.

Abbreviations

UAE

Ultrasound assisted extraction

CE

Cloud extraction

LCA

Life cycle assessment

GWP

Global warming potential

MPP

Marigold petals powder

References

Alves-Rodrigues, A., and A. Shao. 2004. “The Science Behind Lutein.” Toxicology Letters 150 (1): 57–83. https://doi.org/10.1016/j.toxlet.2003.10.031.Search in Google Scholar PubMed

Badday, A. S., A. Z. Abdullah, and K. T. Lee. 2013. “Ultrasound-Assisted Transesterification of Crude Jatropha Oil Using Alumina-Supported Heteropolyacid Catalyst.” Applied Energy 105: 380–8. https://doi.org/10.1016/j.apenergy.2013.01.028.Search in Google Scholar

Bąk, A., and W. Podgórska. 2016. “Interfacial and Surface Tensions of Toluene/Water and Air/Water Systems With Nonionic Surfactants Tween 20 and Tween 80.” Colloids and Surfaces A: Physicochemical and Engineering Aspects 504: 414–25. https://doi.org/10.1016/j.colsurfa.2016.05.091.Search in Google Scholar

Boonnoun, P., P. Tunyasitikun, W. Clowutimon, and A. Shotipruk. 2017. “Production of Free Lutein by Simultaneous Extraction and De-Esterification of Marigold Flowers in Liquefied Dimethyl Ether (DME)–KOH–EtOH Mixture.” Food and Bioproducts Processing 106: 193–200. https://doi.org/10.1016/j.fbp.2017.10.002.Search in Google Scholar

Byrne, F. P., S. Jin, G. Paggiola, T. H. M. Petchey, J. H. Clark, T. J. Farmer, A. J. Hunt, C. Robert McElroy, and J. Sherwood. 2016. “Tools and Techniques for Solvent Selection: Green Solvent Selection Guides.” Sustainable Chemical Processes 4 (1): 1–24. https://doi.org/10.1186/s40508-016-0051-z.Search in Google Scholar

Craft’, N. E., and J. H. Soares. 1992. “Relative Solubility, Stability, and Absorptivity of Lutein and @-Carotene in Organic Solvents.” Journal of Agricultural and Food Chemistry 40: 2, https://doi.org/10.1021/jf00015a013.Search in Google Scholar

CRODA. 2020. Sustainable Bio-Based Surfactants. CRODA. https://media.knowde.com/image/upload/v1616232664/production/Collateral/241527/Sustainable-Bio-based-Surfactants-8pp-01-20-v7-WEB1.Search in Google Scholar

Derrien, M., A. Badr, A. Gosselin, Y. Desjardins, and P. Angers. 2017. “Optimization of a Green Process for the Extraction of Lutein and Chlorophyll From Spinach By-Products Using Response Surface Methodology (RSM).” LWT – Food Science and Technology 79: 170–7. https://doi.org/10.1016/j.lwt.2017.01.010.Search in Google Scholar

Dutra-Molino, J. V., V. Araujo Feitosa, L. C. de Lencastre-Novaes, V. de Carvalho Santos-Ebinuma, A. Moreni Lopes, A. Faustino Jozala, D. de Aráujo Viana Marques, L. Pellegrini Malpiedi, and A. Pessoa. 2014. “Biomolecules Extracted by ATPS: Practical Examples.” Revista Mexicana de Ingeniera Quimica 13 (2): 359–77.Search in Google Scholar

Dwyer, J. H., M. Navab, K. M. Dwyer, K. Hassan, P. Sun, A. Shircore, S. Hama-Levy, et al.. 2001. “Oxygenated Carotenoid Lutein and Progression of Early Atherosclerosis: The Los Angeles Atherosclerosis Study.” Circulation 103 (24): 2922–7. https://doi.org/10.1161/01.CIR.103.24.2922.Search in Google Scholar

Enokida, Y., S. Abd El-Fatah, and C. M. Wai. 2002. “Ultrasound-Enhanced Dissolution of UO2 in Supercritical CO2 Containing a CO2-Philic Complexant of Tri-n-Butylphosphate and Nitric Acid.” Industrial & Engineering Chemistry Research 41 (9): 2282–6. https://doi.org/10.1021/ie010761q.Search in Google Scholar

Eze, O. F., A. Chatzifragkou, and D. Charalampopoulos. 2022. “Properties of Protein Isolates Extracted by Ultrasonication From Soybean Residue (Okara).” Food Chemistry 368: 130837. https://doi.org/10.1016/j.foodchem.2021.130837.Search in Google Scholar PubMed

Fu, Y., Y. Wang, L. Yi, J. Liu, S. Yang, B. Liu, F. Chen, and H. Sun. 2023. “Lutein Production From Microalgae: A Review.” Bioresource Technology 376: 128875. https://doi.org/10.1016/j.biortech.2023.128875.Search in Google Scholar PubMed

Gao, Y., B. Nagy, X. Liu, B. Simándi, and Q. Wang. 2009. “Supercritical CO2 Extraction of Lutein Esters From Marigold (Tagetes erecta L.) Enhanced by Ultrasound.” The Journal of Supercritical Fluids 49 (3): 345–50. https://doi.org/10.1016/j.supflu.2009.02.006.Search in Google Scholar

Gavankar, S., S. Suh, and A. A. Keller. 2015. “The Role of Scale and Technology Maturity in Life Cycle Assessment of Emerging Technologies: A Case Study on Carbon Nanotubes.” Journal of Industrial Ecology 19 (1): 51–60. https://doi.org/10.1111/jiec.12175.Search in Google Scholar

Gogate, P. R., V. S. Sutkar, and A. B. Pandit. 2011. “Sonochemical Reactors: Important Design and Scale Up Considerations With a Special Emphasis on Heterogeneous Systems.” Chemical Engineering Journal 166 (3): 1066–82. https://doi.org/10.1016/j.cej.2010.11.069.Search in Google Scholar

Goula, A. M., M. Ververi, A. Adamopoulou, and K. Kaderides. 2017. “Green Ultrasound-Assisted Extraction of Carotenoids From Pomegranate Wastes Using Vegetable Oils.” Ultrasonics Sonochemistry 34: 821–30. https://doi.org/10.1016/j.ultsonch.2016.07.022.Search in Google Scholar PubMed

Hlaváč, P., M. Božiková, A. Petrović, V. Ardonová, and P. Kotoulek. 2019. “Temperature and Storing Time Effect on Rheologic Properties of Selected Tomato Ketchups.” Journal on Processing and Energy in Agriculture 23 (2): 78–82. https://doi.org/10.5937/jpea1902078h.Search in Google Scholar

Hu, A. J., S. Zhao, H. Liang, T. Qiu Qiu, and G. Chen. 2007. “Ultrasound Assisted Supercritical Fluid Extraction of Oil and Coixenolide From Adlay Seed.” Ultrasonics Sonochemistry 14 (2): 219–24. https://doi.org/10.1016/j.ultsonch.2006.03.005.Search in Google Scholar PubMed

ISO (International Organization for Standardization). 2006. Environmental Management – Life Cycle Assessment – Principles and Framework (ISO 14040(2006)) (Issue 2006-07-01).Search in Google Scholar

Jalali-Jivan, M., and S. Abbasi. 2020. “Novel Approach for Lutein Extraction: Food Grade Microemulsion Containing Soy Lecithin & Sunflower Oil.” Innovative Food Science and Emerging Technologies 66: 102505. https://doi.org/10.1016/j.ifset.2020.102505.Search in Google Scholar

Kaderides, K., A. M. Goula, and K. G. Adamopoulos. 2015. “A Process for Turning Pomegranate Peels Into a Valuable Food Ingredient Using Ultrasound-Assisted Extraction and Encapsulation.” Innovative Food Science and Emerging Technologies 31: 204–15. https://doi.org/10.1016/j.ifset.2015.08.006.Search in Google Scholar

Kamal, I., C. Besombes, and K. Allaf. 2014. “One-Step Processes for In Situ Transesterification to Biodiesel and Lutein Extraction From Microalgae Phaeodactylum Using Instant Controlled Pressure Drop (DIC).” Green Processing and Synthesis 3 (6): 431–40. https://doi.org/10.1515/gps-2014-0047.Search in Google Scholar

Kazibwe, Z., D. Kim, S. Chun, and J. Gopal. 2017. “Ultrasonication Assisted Ultrafast Extraction of Tagetes erecta in Water: Cannonading Antimicrobial, Antioxidant Components.” Journal of Molecular Liquids 229: 453–8. https://doi.org/10.1016/j.molliq.2016.12.044.Search in Google Scholar

Kha, T. C., M. H. Nguyen, P. D. Roach, and C. E. Stathopoulos. 2013. “Effect of Drying Pre-Treatments on the Yield and Bioactive Content of Oil Extracted From Gac Aril.” International Journal of Food Engineering 10 (1): 103–12. https://doi.org/10.1515/ijfe-2013-0028.Search in Google Scholar

Khanpit, V. V., S. P. Tajane, and S. A. Mandavgane. 2022a. “Orange Waste Peel to High Value Soluble Dietary Fiber Concentrate: Comparison of Conversion Methods and Their Environmental Impact.” Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-022-02481-6.Search in Google Scholar

Khanpit, V. V., S. P. Tajane, and S. A. Mandavgane. 2022b. “Production of Soluble Dietary Fiber Concentrate From Waste Orange Peels: Study of Nutritional and Physicochemical Properties and Life Cycle Assessment.” Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-022-03007-w.Search in Google Scholar

Khanpit, V. V., S. P. Tajane, and S. A. Mandavgane. 2023. “Technoeconomic and Life Cycle Analysis of Soluble Dietary Fiber Concentrate Production From Waste Orange Peels.” Waste Management 155: 29–39. https://doi.org/10.1016/j.wasman.2022.10.036.Search in Google Scholar PubMed

Koneva, A. S., E. A. Safonova, P. S. Kondrakhina, M. A. Vovk, A. A. Lezov, Y. S. Chernyshev, and N. A. Smirnova. 2017. “Effect of Water Content on Structural and Phase Behavior of Water-In-Oil (n-Decane) Microemulsion System Stabilized by Mixed Nonionic Surfactants SPAN 80/TWEEN 80.” Colloids and Surfaces A: Physicochemical and Engineering Aspects 518: 273–82. https://doi.org/10.1016/j.colsurfa.2017.01.020.Search in Google Scholar

Lakshmi, A., G. Aishwarya, S. Priya, S. Rlc, S. Raju, and V. Rao. 2019. “Extraction and Biological Evaluation of Esterfied Lutein From Marigold Flower Petals.” Journal of Pharmacognosy and Phytochemistry 1 (1): 2523–6075. www.chemicaljournals.com.Search in Google Scholar

Landrum, J. T., and R. A. Bone. 2001. “Lutein, Zeaxanthin, and the Macular Pigment.” Archives of Biochemistry and Biophysics 385 (1): 28–40. https://doi.org/10.1006/abbi.2000.2171.Search in Google Scholar PubMed

Li, D. J., J. F. Song, A. Q. Xu, and C. Q. Liu. 2014. “Optimization of the Ultrasound-Assisted Synthesis of Lutein Disuccinate Using Uniform Design.” Ultrasonics Sonochemistry 21 (1): 98–103. https://doi.org/10.1016/j.ultsonch.2013.06.004.Search in Google Scholar PubMed

Ma, Y., X. Wu, L. Zhao, Y. Wang, and X. Liao. 2019. “Comparison of the Compounds and Characteristics of Pepper Seed Oil by Pressure-Assisted, Ultrasound-Assisted and Conventional Solvent Extraction.” Innovative Food Science and Emerging Technologies 54: 78–86. https://doi.org/10.1016/j.ifset.2019.03.011.Search in Google Scholar

Madadi, B., G. Pazuki, and B. Nasernejad. 2013. “Partitioning of Cefazolin in Biocompatible Aqueous Biphasic Systems Based on Surfactant.” Journal of Chemical and Engineering Data 58 (10): 2785–92. https://doi.org/10.1021/je4004756.Search in Google Scholar

Maheshwari, N., R. K. Arya, G. D. Verros, P. B. Dhamole, and A. Kannan. 2023. “Surfactant-Enhanced Extraction of Lutein From Marigold Petals Using an Aqueous Two-Phase System.” Separations 10 (2): 7–8, https://doi.org/10.3390/separations10020133.Search in Google Scholar

Manzoor, S., R. Rashid, B. Prasad Panda, V. Sharma, and M. Azhar. 2022. “Green Extraction of Lutein From Marigold Flower Petals, Process Optimization and its Potential to Improve the Oxidative Stability of Sunflower Oil.” Ultrasonics Sonochemistry 85: 105994. https://doi.org/10.1016/j.ultsonch.2022.105994.Search in Google Scholar PubMed PubMed Central

Melo, R. P. F., E. L. Barros Neto, M. C. P. A. Moura, T. N. Castro Dantas, A. A. Dantas Neto, and H. N. M. Oliveira. 2014. “Removal of Reactive Blue 19 Using Nonionic Surfactant in Cloud Point Extraction.” Separation and Purification Technology 138: 71–6. https://doi.org/10.1016/j.seppur.2014.10.009.Search in Google Scholar

Moharkar, S., P. B. Dhamole, and V. L. Gole. 2022. “Integrated Ultrasound-Mediated Sugaring-Out Extraction of Erythromycin From Fermentation Broth.” Separation and Purification Technology 278: 119517, https://doi.org/10.1016/j.seppur.2021.119517.Search in Google Scholar

Ochoa Becerra, M., L. Mojica Contreras, M. Hsieh Lo, J. Mateos Díaz, and G. Castillo Herrera. 2020. “Lutein as a Functional Food Ingredient: Stability and Bioavailability.” Journal of Functional Foods 66: 103771. https://doi.org/10.1016/j.jff.2019.103771.Search in Google Scholar

Papoutsis, K., P. Pristijono, J. B. Golding, C. E. Stathopoulos, M. C. Bowyer, C. J. Scarlett, and Q. V. Vuong. 2018. “Optimizing a Sustainable Ultrasound-Assisted Extraction Method for the Recovery of Polyphenols From Lemon By-Products: Comparison With Hot Water and Organic Solvent Extractions.” European Food Research and Technology 244 (8): 1353–65. https://doi.org/10.1007/s00217-018-3049-9.Search in Google Scholar

Patil, S. S., A. Pathak, and V. K. Rathod. 2021. “Ultrasonics – Sonochemistry Optimization and Kinetic Study of Ultrasound Assisted Deep Eutectic Solvent Based Extraction: A Greener Route for Extraction of Curcuminoids From Curcuma Longa.” Ultrasonics Sonochemistry 70: 105267. https://doi.org/10.1016/j.ultsonch.2020.105267.Search in Google Scholar PubMed PubMed Central

Prabhu, A., K. S. Abdul, and P.-D. Rekha. 2015. “Isolation and Purification of Lutein From Indian Spinach Basella alba.” Research Journal of Pharmacy and Technology 8 (10): 1379. https://doi.org/10.5958/0974-360x.2015.00247.4.Search in Google Scholar

Prieto, C., and L. Calvo. 2013. “Performance of the Biocompatible Surfactant Tween 80, for the Formation of Microemulsions Suitable for New Pharmaceutical Processing.” Journal of Applied Chemistry 2013: 1–10. https://doi.org/10.1155/2013/930356.Search in Google Scholar

Sharma, O. P., and T. K. Bhat. 2009. “DPPH Antioxidant Assay Revisited.” Food Chemistry 113 (4): 1202–5. https://doi.org/10.1016/j.foodchem.2008.08.008.Search in Google Scholar

Siriamornpun, S., O. Kaisoon, and N. Meeso. 2012. “Changes in Colour, Antioxidant Activities and Carotenoids (Lycopene, β-Carotene, Lutein) of Marigold Flower (Tagetes erecta L.) Resulting From Different Drying Processes.” Journal of Functional Foods 4 (4): 757–66. https://doi.org/10.1016/j.jff.2012.05.002.Search in Google Scholar

Tan, P. W., C. P. Tan, and C. W. Ho. 2011. “Antioxidant Properties: Effects of Solid-To-Solvent Ratio on Antioxidant Compounds and Capacities of Pegaga (Centella Asiatica).” International Food Research Journal 18 (2): 557–62.Search in Google Scholar

Tan, C. X., G. H. Chong, H. Hamzah, and H. M. Ghazali. 2018. “Comparison of Subcritical CO2 and Ultrasound-Assisted Aqueous Methods With the Conventional Solvent Method in the Extraction of Avocado Oil.” The Journal of Supercritical Fluids 135: 45–51. https://doi.org/10.1016/j.supflu.2017.12.036.Search in Google Scholar

Wang, M., R. Tsao, S. Zhang, Z. Dong, R. Yang, J. Gong, and Y. Pei. 2006. “Antioxidant Activity, Mutagenicity/Anti-Mutagenicity, and Clastogenicity/Anti-Clastogenicity of Lutein From Marigold Flowers.” Food and Chemical Toxicology 44 (9): 1522–9. https://doi.org/10.1016/j.fct.2006.04.005.Search in Google Scholar PubMed

Wang, T., J. Han, Y. Tian, D. Zhang, Y. Wang, Y. Wu, and L. Ni. 2016. “Combined Process of Reaction, Extraction, and Purification of Lutein in Marigold Flower by Isopropanol–KOH Aqueous Two-Phase System.” Separation Science and Technology 51 (9): 1490–8. https://doi.org/10.1080/01496395.2016.1166136.Search in Google Scholar

Yang, Z., X. Mao, J. Cui, Y. Wang, and Y. Zhang. 2021. “Enhancement and Analysis of Anthracene Degradation by Tween 80 in LMS-HOBt.” Scientific Reports 11 (1): 1–11. https://doi.org/10.1038/s41598-021-90609-5.Search in Google Scholar PubMed PubMed Central

Received: 2023-03-11
Accepted: 2023-08-22
Published Online: 2023-11-03

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/ijcre-2023-0054
Keywords for this article
lutein; biocompatible surfactants; ultrasound assisted extraction; antioxidant

Articles in the same Issue

  1. Frontmatter
  2. Special Issue Articles
  3. An experimental evaluation of green surfactants to stabilize silica nanofluids in saline conditions and its application in CO2 absorption
  4. Indoor air quality control using lab scale air purifier tower
  5. Green ultrasound-assisted extraction and life cycle assessment of lutein from marigold flowers using biocompatible surfactants
  6. Numerical analysis of various shapes of lozenge pin-fins in microchannel heat sink
  7. Extraction of biodiesel from pomelo peel and investigation of its efficiency as a lubricant in water-based drilling fluid
  8. Methyl-orange/reduced graphene oxide composite as the electrode material for the solid-state supercapacitor
  9. Efficiency and environmental stability of TiO2 based solar cells for green electricity production
  10. Validating experimental data for attenuation coefficients of developed polymer composites in shielding applications through Monte Carlo simulation
  11. Experimental studies on renewable hydrogen production by steam reforming of glycerol over zirconia promoted on Ni/Al2O3 catalyst
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