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LINC00858 facilitates formation of hepatic metastases from colorectal cancer via regulating the miR-132-3p/IGF2BP1 axis

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Published/Copyright: March 2, 2023
Biological Chemistry
From the journal Biological Chemistry Volume 405 Issue 2

Abstract

Hepatic metastasis is a major cause of colorectal cancer (CRC)-related deaths. Presently, the role of long non-coding RNAs (lncRNAs) in hepatic metastases from CRC is elusive. We dissected possible interplay between LINC00858/miR-132-3p/IGF2BP1 via bioinformatics approaches. Subsequently we analyzed mRNA expression of LINC00858, miR-132-3p and IGF2BP1 through qRT-PCR. Western blot was used to detect protein expression of IGF2BP1. RNA immunoprecipitation chip and dual-luciferase assay validated interaction between LINC00858 and miR-132-3p, as well as miR-132-3p and IGF2BP1. Cell viability, invasion, and migration were examined via CCK-8, colony formation, transwell and wound healing assays. Effect of LINC00858 on CRC hepatic metastases was validated via in vivo assay. Upregulated LINC00858 and IGF2BP1, and downregulated miR-132-3p were predicted in tumor tissues of patients with hepatic metastases from CRC. There were targeting relationships between LINC00858 and miR-132-3p, as well as miR-132-3p and IGF2BP1. Besides, LINC00858 facilitated progression of CRC cells. Rescue assay suggested that silencing LINC00858 suppressed CRC cell progression, while further silencing miR-132-3p or overexpressing IGF2BP1 reversed such effects. LINC00858 could facilitate CRC tumor growth and hepatic metastases. LINC00858 induced CRC hepatic metastases via regulating miR-132-3p/ IGF2BP1, and this study may deliver a new diagnostic marker for the disease.

Keywords: colorectal cancer; hepatic metastasis; IGF2BP1; LINC00858; miR-132-3p
Corresponding author: Yinggang Chen, Department of Gastrointestinal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital and Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 113 Baohe Road, Shenzhen 518116, Guangdong, Province, China, E-mail:

Funding source: Sanming Project of Medicine in Shenzhen

Award Identifier / Grant number: SZSM201911012

Funding source: Shenzhen High-level Hospital Construction Fund

  1. Research funding: This work was supported by Sanming Project of Medicine in Shenzhen (grant number: SZSM201911012) and Shenzhen High-level Hospital Construction Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

  2. Author contributions: Conception and design: Peng Sun, Yusong Luan. Administrative support: Yinggang Chen, Peide Ren, Peng Sun. Provision of study materials: Xuhao Cai, Qi Liu, Peide Ren, Pengpan Xin. Collection and assembly of data: Yonggang Yu, Bolun Song, Yangyang Wang. Data analysis and interpretation: Huijing Chang, Haoyue Ma, Peng Sun, Yusong Luan. Manuscript writing: All authors. Final approval of manuscript: All authors

  3. Conflict of interest statement: The authors declare that they have no competing interests.

  4. Ethical approval: This study protocol was reviewed and approved by [Cancer Hospital, Chinese Academy of Medical Science], approval number [NCC2016JZ-06].

  5. Data availability: Data cannot be shared for confidentiality reasons. Queries about the data should be directed to the corresponding author.

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Received: 2022-11-15
Accepted: 2023-01-19
Published Online: 2023-03-02
Published in Print: 2024-02-26

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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  2. Research Articles/Short Communications
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https://doi.org/10.1515/hsz-2022-0328
Keywords for this article
colorectal cancer; hepatic metastasis; IGF2BP1; LINC00858; miR-132-3p

Articles in the same Issue

  1. Frontmatter
  2. Research Articles/Short Communications
  3. Protein Structure and Function
  4. Stoichiometry and architecture of the platelet membrane complex glycoprotein Ib-IX-V
  5. Characterization of the active site in the thiocyanate-forming protein from Thlaspi arvense (TaTFP) using EPR spectroscopy
  6. Molecular Medicine
  7. Salvia miltiorrhiza augments endothelial cell function for ischemic hindlimb recovery
  8. Cell Biology and Signaling
  9. LINC00858 facilitates formation of hepatic metastases from colorectal cancer via regulating the miR-132-3p/IGF2BP1 axis
  10. Mechanism of hypoxia-induced exosome circ_0051799 regulating the progression of lung adenocarcinoma
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