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Volatilomes of Cyclocybe aegerita during different stages of monokaryotic and dikaryotic fruiting

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Published/Copyright: February 11, 2020
Biological Chemistry
From the journal Biological Chemistry Volume 401 Issue 8

Abstract

Volatile organic compounds (VOC) are characteristic for different fungal species. However, little is known about VOC changes during development and their biological role. Therefore, we established a laboratory cultivation system in modified crystallizing dishes for analyzing VOC during fruiting body development of the dikaryotic strain Cyclocybe aegerita AAE-3 as well as four monokaryotic offspring siblings exhibiting different fruiting phenotypes. From these, VOC were extracted directly from the headspace (HS) and analyzed by means of gas chromatography-mass spectrometry (GC-MS). For all tested strains, alcohols and ketones, including oct-1-en-3-ol, 2-methylbutan-1-ol and cyclopentanone, were the dominant substances in the HS of early developmental stages. In the dikaryon, the composition of the VOC altered with ongoing fruiting body development and, even more drastically, during sporulation. At the latter stage, sesquiterpenes, especially Δ6-protoilludene, α-cubebene and δ-cadinene, were the dominant substances. After sporulation, the amount of sesquiterpenes decreased, while additional VOC, mainly octan-3-one, appeared. In the HS of the monokaryons, less VOC were present of which all were detectable in the HS of the dikaryon C. aegerita AAE-3. The results of the present study show that the volatilome of C. aegerita changes considerably depending on the developmental stage of the fruiting body.

Keywords: Basidiomycota; fungi; mushroom aroma; sesquiterpenes; SPME-GC-MS; volatile organic compounds

Acknowledgments

This study was financially supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Funder Id: http://dx.doi.org/10.13039/501100001659, Grant Number: RU 2137/1-1. The support by LOEWE (State-Offensive for the Development of Scientific and Economic Excellence) is gratefully acknowledged. The authors thank the two anonymous reviewers for helpful comments and suggestions that helped to improve this paper.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2019-0392).

Received: 2019-10-10
Accepted: 2020-01-09
Published Online: 2020-02-11
Published in Print: 2020-07-28

©2020 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hsz-2019-0392
Keywords for this article
Basidiomycota; fungi; mushroom aroma; sesquiterpenes; SPME-GC-MS; volatile organic compounds

Articles in the same Issue

  1. Frontmatter
  2. Obituary
  3. In memoriam Kanti D. Bhoola (1930–2019)
  4. Reviews
  5. Septin architecture and function in budding yeast
  6. Small-molecule modulation of p53 protein-protein interactions
  7. EZH2 function in immune cell development
  8. Research Articles/Short Communications
  9. Protein Structure and Function
  10. Comprehensive analysis of peptides and low molecular weight components of the giant ant Dinoponera quadriceps venom
  11. Targeting of parvulin interactors by diazirine mediated cross-linking discloses a cellular role of human Par14/17 in actin polymerization
  12. Membranes, Lipids, Glycobiology
  13. Heat shock protein 60 is a disease-associated sialoglycoprotein in human non-small cell lung cancer
  14. Molecular Medicine
  15. Radiofrequency ablation triggers the migration of hepatocellular carcinoma cells by suppressing miR-148a-5p
  16. Cell biology and Signaling
  17. Volatilomes of Cyclocybe aegerita during different stages of monokaryotic and dikaryotic fruiting
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