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The monoheme cytochrome c subunit of Alternative Complex III is a direct electron donor to caa3 oxygen reductase in Rhodothermus marinus

  • Patrícia N. Refojo , Filipa Calisto , Miguel A. Ribeiro , Miguel Teixeira and Manuela M. Pereira EMAIL logo
Published/Copyright: June 24, 2017
Biological Chemistry
From the journal Biological Chemistry Volume 398 Issue 9

Abstract

Alternative Complex III (ACIII) is an example of the robustness and flexibility of prokaryotic respiratory chains. It performs quinol:cytochrome c oxidoreductase activity, being functionally equivalent to the bc1 complex but structurally unrelated. In this work we further explored ACIII investigating the role of its monoheme cytochrome c subunit (ActE). We expressed and characterized the individually isolated ActE, which allowed us to suggest that ActE is a lipoprotein and to show its function as a direct electron donor to the caa3 oxygen reductase.

Keywords: bc1 complex; cytochrome c oxidase; lipoprotein; quinone; respiratory chain

Acknowledgements

P.N.R. and F.C. are recipients of grants from Fundação para a Ciência e a Tecnologia (SFRH/BPD/71022/2010 and SFRH/BD/104481/2014, respectively). The work was funded by Fundação para a Ciência e a Tecnologia (PTDC/BIA-PRO/120949/2010 and IF/01507/2015to MMP) and supported by LISBOA-01-0145-FEDER-007660 co-funded by FEDER through COMPETE2020-POCI and by Fundação para a Ciência e a Tecnologia. MS were obtained by the UniMS – Mass Spectrometry Unit, ITQB/iBET, Oeiras, Portugal. N-terminal amino acid sequence was determined by the Analytical Services Unit, ITQB/iBET, Oeiras, Portugal. Célia M. Silveira and Smilja Todorovic are acknowledged for helping with cyclic voltammetry measurements and Ricardo Louro with the acquisition and analysis of NMR data. Filipe M. Sousa is acknowledged for the critical reading.

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Received: 2016-11-2
Accepted: 2017-1-23
Published Online: 2017-6-24
Published in Print: 2017-8-28

©2017 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hsz-2016-0323
Keywords for this article
bc1 complex; cytochrome c oxidase; lipoprotein; quinone; respiratory chain

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. The role of sirtuins in mitochondrial function and doxorubicin-induced cardiac dysfunction
  4. Structural and functional insight into pan-endopeptidase inhibition by α2-macroglobulins
  5. Iron overload and altered iron metabolism in ovarian cancer
  6. Shared function and moonlighting proteins in molybdenum cofactor biosynthesis
  7. Minireview
  8. Molecular challenges imposed by MHC-I restricted long epitopes on T cell immunity
  9. Research Articles/Short Communications
  10. Protein Structure and Function
  11. The monoheme cytochrome c subunit of Alternative Complex III is a direct electron donor to caa3 oxygen reductase in Rhodothermus marinus
  12. Cell Biology and Signaling
  13. β-NGF and β-NGF receptor upregulation in blood and synovial fluid in osteoarthritis
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