Abstract
OrysaEULD1A is one of the five EUL genes in rice (Oryza sativa) encoding a putative carbohydrate-binding protein belonging to the family of Euonymus related lectins (EUL). The OrysaEULD1A sequence comprises two highly similar EUL domains (91% sequence similarity and 72% sequence identity) separated by a 23 amino acid linker sequence and preceded by a 19 amino acid N-terminal sequence. In the present study, the full-length protein OrysaEULD1A as well as its individual domains OrysaEULD1A domain 1 and 2 were expressed in Pichiapastoris. After purification of the recombinant proteins, their carbohydrate-binding specificity was analyzed and compared. Interestingly, all recombinant lectins showed clear specificity towards galactosylated structures. Furthermore, all recombinant proteins agglutinated red blood cells, indicating that the full-length protein OrysaEULD1A and its domains are true lectins. These results taken together with data previously reported for single-domain EUL proteins indicate that although the amino acids – responsible for the formation of the carbohydrate-binding site – are identical for all EUL proteins in rice, these lectins show different carbohydrate specificities. This promiscuity of the carbohydrate-binding site can be attributed to gene divergence.
Acknowledgments
This work was funded primarily by the Fund for Scientific Research—Flanders (FWO grant G.0022.08) and the Research Council of Ghent University (project BOF10/GOA/003). Bassam Al Atalah is a recipient of a doctoral grant from the Special Research Council of Ghent University. Dr. Dieter Vanderschaeghe is a postdoctoral fellow of FWO (Fonds Wetenschappelijk Onderzoek Vlaanderen). Yehudi Bloch is predoctoral fellow of the IWT-Flanders (Belgium).
References
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Articles in the same Issue
- Masthead
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Articles in the same Issue
- Masthead
- Masthead
- Reviews
- Physiological and pathological relevance of secretory microRNAs and a perspective on their clinical application
- Integration of stress signals by homeodomain interacting protein kinases
- Citrate – new functions for an old metabolite
- Research Articles/Short Communications
- Protein Structure and Function
- Azobenzene switch with a long-lived cis-state to photocontrol the enzyme activity of a histone deacetylase-like amidohydrolase
- Characterization of a type D1A EUL-related lectin from rice expressed in Pichia pastoris
- The legless lizard Anguis fragilis (slow worm) has a potent metal-responsive transcription factor 1 (MTF-1)
- Cell Biology and Signaling
- Effects of short-term chemical ablation of glucagon signalling by peptide-based glucagon receptor antagonists on insulin secretion and glucose homeostasis in mice
- A potent soluble epoxide hydrolase inhibitor, t-AUCB, modulates cholesterol balance and oxidized low density lipoprotein metabolism in adipocytes in vitro
- Activation of GPR119 by fatty acid agonists augments insulin release from clonal β-cells and isolated pancreatic islets and improves glucose tolerance in mice