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Lipid-lowering effect of molluscan (Katelysia opima) glycosaminoglycan (GAG) in hypercholesterolemic induced rats

  • Vijayabaskar Pandian EMAIL logo , Natarajan Aravindan , Sethupathy Subramanian and Somasundaram T. Somasundaran
Published/Copyright: October 22, 2013
Biological Chemistry
From the journal Biological Chemistry Volume 395 Issue 3

Abstract

Identifying pharmacologically safe lipid-lowering ‘deliverables’ could potentiate therapeutic outcome for diet-induced atherogenesis. Accordingly, we investigated the potential of molluscan (Katelysia opima) glycosaminoglycan (GAG) in modulating the early lipid changes in atherogenesis. Wistar rats were fed a diet with (n=24) or without (n=6) hypercholesterolemic atherogenic CCT (rat chow supplemented with 4% cholesterol, 1% cholic acid, and 0.5% thiouracil) for 17 days. CCT-fed rates were (i) treated with isolated molluscan GAG (40 mg/kg/day, s.c.) for 10 days after the introduction of CCT diet, (ii) cotreated with GAG (40 mg/kg/day, s.c.) for 17 days, or (iii) treated with heparin (200 units/kg/day, s.c.) for 10 days after the introduction of CCT. The increases induced by CCT diet in the plasma levels of cholesterol, triglycerides, high-density lipoprotein, very-low-density lipoprotein, and low-density lipoprotein were completely attenuated with GAG treatment. Consistently, alterations induced by CCT diet in the levels of plasma lecithin cholesterol acyltransferase and lipoprotein lipase activities were restored to baseline levels with GAG treatment. Coherently, histology revealed a decrease associated with GAG treatment in the CCT-diet-induced foam cells (in aorta), tubular damages (kidney), and lipid accumulations (liver). Together, these results suggest that GAG may exert antiatherogenesis potential by significantly attenuating lipid modulations derived by a high-fat diet. Further, the data imply that the GAG extracts may comprehensively prevent hypercholesterolemia-associated tissue damage and could thus serve as a therapeutic deliverable for hypercholesterolemia.

Keywords: atherosclerosis; CCT diet; heparin; LCAT-LPL; molluscan GAG
Corresponding author: Vijayabaskar Pandian, Department of Radiation Oncology, University of Oklahoma Health Sciences Center, 940 Stanton L. Young Boulevard, Oklahoma City, OK 73104, USA; and Faculty of Marine Sciences, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, Tamilnadu, India, e-mail:

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Received: 2013-7-1
Accepted: 2013-10-19
Published Online: 2013-10-22
Published in Print: 2014-03-01

©2014 by Walter de Gruyter Berlin Boston

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Guest Editorial
  4. Highlight issue: membranes in motion
  5. HIGHLIGHT: MOSBACH COLLOQUIUM 2013 ‘MEMBRANES IN MOTION’
  6. Remodeling of membrane compartments: some consequences of membrane fluidity
  7. Reshaping biological membranes in endocytosis: crossing the configurational space of membrane-protein interactions
  8. Novel intracellular functions of apolipoproteins: the ApoO protein family as constituents of the Mitofilin/MINOS complex determines cristae morphology in mitochondria
  9. Membranes in motion: mitochondrial dynamics and their role in apoptosis
  10. Crosstalk of lipid and protein homeostasis to maintain membrane function
  11. Endocytic Rabs in membrane trafficking and signaling
  12. Review
  13. Plasmacytoid dendritic cells and autoimmune inflammation
  14. Research Articles/Short Communications
  15. Protein Structure and Function
  16. Molecular interactions of hemoglobin with resveratrol: potential protective antioxidant role and metabolic adaptations of the erythrocyte
  17. Membranes, Lipids, Glycobiology
  18. Lipid-lowering effect of molluscan (Katelysia opima) glycosaminoglycan (GAG) in hypercholesterolemic induced rats
https://doi.org/10.1515/hsz-2013-0214
Keywords for this article
atherosclerosis; CCT diet; heparin; LCAT-LPL; molluscan GAG

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Guest Editorial
  4. Highlight issue: membranes in motion
  5. HIGHLIGHT: MOSBACH COLLOQUIUM 2013 ‘MEMBRANES IN MOTION’
  6. Remodeling of membrane compartments: some consequences of membrane fluidity
  7. Reshaping biological membranes in endocytosis: crossing the configurational space of membrane-protein interactions
  8. Novel intracellular functions of apolipoproteins: the ApoO protein family as constituents of the Mitofilin/MINOS complex determines cristae morphology in mitochondria
  9. Membranes in motion: mitochondrial dynamics and their role in apoptosis
  10. Crosstalk of lipid and protein homeostasis to maintain membrane function
  11. Endocytic Rabs in membrane trafficking and signaling
  12. Review
  13. Plasmacytoid dendritic cells and autoimmune inflammation
  14. Research Articles/Short Communications
  15. Protein Structure and Function
  16. Molecular interactions of hemoglobin with resveratrol: potential protective antioxidant role and metabolic adaptations of the erythrocyte
  17. Membranes, Lipids, Glycobiology
  18. Lipid-lowering effect of molluscan (Katelysia opima) glycosaminoglycan (GAG) in hypercholesterolemic induced rats
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