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The amino acids surrounding the flavin 7a-methyl group determine the UVA spectral features of a LOV protein

  • Sarah Raffelberg , Alexander Gutt , Wolfgang Gärtner , Carmen Mandalari , Stefania Abbruzzetti , Cristiano Viappiani and Aba Losi EMAIL logo
Published/Copyright: July 3, 2013
Biological Chemistry
From the journal Biological Chemistry Volume 394 Issue 11

Abstract

Flavin-binding light, oxygen, and voltage (LOV) domains are UVA/blue-light-sensing protein units that form a reversible flavin mononucleotide-cysteine adduct upon light induction. In their dark-adapted state, LOV domains exhibit the typical spectral features of fully oxidized riboflavin derivatives. A survey on the absorption spectra of various LOV domains revealed that the UVA spectral range is the most variable region (whereas the absorption band at 450 nm is virtually unchanged), showing essentially two distinct patterns found in plant phototropin LOV1 and LOV2 domains, respectively. In this work, we have identified a residue directly interacting with the isoalloxazine methyl group at C(7a) as the major UVA spectral tuner. In YtvA from Bacillus subtilis, this amino acid is threonine 30, and its mutation into apolar residues converts the LOV2-like spectrum of native YtvA into a LOV1-like pattern. Mutation T30A also accelerates the photocycle ca. 4-fold. Together with control mutations at different positions, our results experimentally confirm the previously calculated direction of the transition dipole moment for the UVA ππ* state and identify the mechanisms underlying spectral tuning in the LOV domains.

Keywords: photobiophysics; photochemistry; spectral tuning; UVA/blue-light sensing; YtvA
Corresponding author: Aba Losi, Department of Physics and Earth Sciences, University of Parma (www.unipr.it), Viale G.P. Usberti 7/A, I-43124, Parma, Italy, e-mail:

S.R. is a recipient of a PhD student grant from the ‘Biostruct’ program of the Heinrich-Heine-University Düsseldorf. This work has been partially supported by the Vigoni program (to A.L. and W.G.) and the University of Parma (fellowship to C.M.). We thank Francesca Pennacchietti for the solvatochromic data on FMN reported within the supplementary material.

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Received: 2013-4-23
Accepted: 2013-7-1
Published Online: 2013-07-03
Published in Print: 2013-11-01

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/hsz-2013-0163
Keywords for this article
photobiophysics; photochemistry; spectral tuning; UVA/blue-light sensing; YtvA

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Guest Editorial
  4. Highlight: NRW Research School BioStruct – Biological Structures in Molecular Medicine and Biotechnology
  5. Highlight: NRW Research School Biostruct – Biological Structures in Molecular Medicine and Biotechnology
  6. Structural features of antiviral DNA cytidine deaminases
  7. Molecular insights into type I secretion systems
  8. Structural comparison of the transport units of type V secretion systems
  9. Rho-kinase: regulation, (dys)function, and inhibition
  10. Role of centrosomal adaptor proteins of the TACC family in the regulation of microtubule dynamics during mitotic cell division
  11. Revisiting Disrupted-in-Schizophrenia 1 as a scaffold protein
  12. Structural snapshot of cyclic nucleotide binding domains from cyclic nucleotide-sensitive ion channels
  13. Full-length Vpu and human CD4(372–433) in phospholipid bilayers as seen by magic angle spinning NMR
  14. Membrane protein stability depends on the concentration of compatible solutes – a single molecule force spectroscopic study
  15. Expression and characterisation of fully posttranslationally modified cellular prion protein in Pichia pastoris
  16. Contribution of distinct platelet integrins to binding, unfolding, and assembly of fibronectin
  17. Shear-related fibrillogenesis of fibronectin
  18. Enzyme-substrate complexes of the quinate/shikimate dehydrogenase from Corynebacterium glutamicum enable new insights in substrate and cofactor binding, specificity, and discrimination
  19. The amino acids surrounding the flavin 7a-methyl group determine the UVA spectral features of a LOV protein
  20. Determinants of the species selectivity of oxazolidinone antibiotics targeting the large ribosomal subunit
  21. NSR from Streptococcus agalactiae confers resistance against nisin and is encoded by a conserved nsr operon
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