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Synthesis of a novel benzodifuran derivative and its molecular recognition of poly rA RNA

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Veröffentlicht/Copyright: 29. Mai 2013

Abstract

In this manuscript, we describe a synthetic approach to a novel benzodifuran derivative as well as CD studies regarding its ability to interact with DNA and RNA. After the chemical synthesis and ESI-MS and NMR characterization, this heteroaromatic molecule was investigated by CD spectroscopy in order to evaluate it as a potential nucleic acid binder. Interestingly, the benzodifuran compound was found to be able to induce conformational changes in both DNA and RNA homoadenine molecules forming in the latter case a complex with a 6:1 benzodifuran/nucleobase stoichiometric ratio, as evidenced by CD titration experiments.

Keywords: benzodifuran; CD; RNA

Corresponding author: Giovanni N. Roviello, Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, I-80134 Napoli, Italy

We thank Dr. Giuseppe Perretta and Mr. Antonio Liccardo for their technical support, as well as the institutions that supported our laboratory (Consiglio Nazionale delle Ricerche and Università degli Studi di Napoli ‘Federico II’).

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Received: 2013-3-26
Accepted: 2013-5-28
Published Online: 2013-05-29
Published in Print: 2013-09-01

©2013 by Walter de Gruyter Berlin Boston

Heruntergeladen am 8.5.2026 von https://www.degruyterbrill.com/document/doi/10.1515/hsz-2013-0154/html?lang=de
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