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Hydrolysis of dipeptide derivatives reveals the diversity in the M49 family

  • Nina Jajčanin-Jozić and Marija Abramić EMAIL logo
Published/Copyright: January 28, 2013
Biological Chemistry
From the journal Biological Chemistry Volume 394 Issue 6

Abstract

Dipeptidyl peptidase III, a metallopeptidase of the M49 family, was first identified (in the pituitary) by its specific cleavage of diarginyl arylamides, which have been used as preferred assay substrates until now. Here we examined the activity of the yeast and human dipeptidyl peptidase III in parallel. The human enzyme preferred Arg2-β-naphthylamide and showed 620-fold higher kcat/Km for this substrate. In contrast, the yeast enzyme did not display a preference for any of the X-Arg-β-naphthylamide analyzed. The replacement of Gly505 with Asp, resulted in a less active, but more selective, yeast enzyme form. These results indicate diversity in cleavage specificity in the M49 family.

Keywords: arylamide substrate; dipeptidyl peptidase III; Saccharomyces cerevisiae; site-directed mutagenesis; zinc-peptidase
Corresponding author: Marija Abramić, Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10002 Zagreb, Croatia

Support for this study by the Croatian Ministry of Science, Education and Sport (Project 098-1191344-2938) is gratefully acknowledged.

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Received: 2012-12-6
Accepted: 2013-1-14
Published Online: 2013-01-28
Published in Print: 2013-06-01

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/hsz-2012-0347
Keywords for this article
arylamide substrate; dipeptidyl peptidase III; Saccharomyces cerevisiae; site-directed mutagenesis; zinc-peptidase

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Reviews
  4. The unique activity of bone morphogenetic proteins in bone: a critical role of the Smad signaling pathway
  5. The formate/nitrite transporter family of anion channels
  6. The human Ah receptor: hints from dioxin toxicities to deregulated target genes and physiological functions
  7. Heparan sulfate: a key regulator of embryonic stem cell fate
  8. Research Articles/Short Communications
  9. Genes and Nucleic Acids
  10. Expression and translation of the COX-1b gene in human cells – no evidence of generation of COX-1b protein
  11. Protein Structure and Function
  12. Evaluation of the metal binding sites in a recombinant coagulation factor VIII identifies two sites with unique metal binding properties
  13. Hydrolysis of dipeptide derivatives reveals the diversity in the M49 family
  14. Molecular Medicine
  15. Betulinic acid suppresses NGAL-induced epithelial-to-mesenchymal transition in melanoma
  16. Proteolysis
  17. Influence of partial unfolding and aggregation of human stefin B (cystatin B) EPM1 mutants G50E and Q71P on selective cleavages by cathepsins B and S
  18. The Staphylococcus aureus leucine aminopeptidase is localized to the bacterial cytosol and demonstrates a broad substrate range that extends beyond leucine
  19. Book Review
  20. Extracellular Matrix: Pathobiology and Signaling
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