Aminoquinolines as fluorescent labels for hydrophilic interaction liquid chromatography of oligosaccharides
-
Weston B. Struwe
and
Pauline M. Rudd
Abstract
In this study, we investigated the potential of four different aminoquinoline (AQ) compounds as fluorescent labels for glycan analysis using hydrophilic interaction liquid chromatography (HILIC) and fluorescence detection (FLD). We confirmed the optimal excitation and emission wavelengths of 3-AQ and 6-AQ conjugated to glycan standards using three-dimensional fluorescent spectral scanning. The optimal excitation and emission wavelengths for 6-AQ were confirmed at λex=355 nm and λem=440 nm. We concluded that the optimal wavelengths for 3-AQ were λex=355 nm and λem=420 nm, which differed considerably from the wavelengths applied in previous reports. HILIC-FLD chromatograms using experimentally determined wavelengths were similar to 2-aminobenzamide controls, but the peak capacity and resolution differed significantly when published 3-AQ λex/em values were applied. Furthermore, we found that 5-AQ and 8-AQ labeled maltohexaose did not display any fluorescent pro\xadperties when used as a carbohydrate tag for HPLC analysis. Finally, we applied experimentally determined wavelengths to 3-AQ labeled N-glycans released from human IgG to illustrate changes in retention time as well as to demonstrate that AQ labeling is applicable to complex sample analysis via exoglycosidase sequencing.
©2012 by Walter de Gruyter Berlin Boston
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- The analysis of N-glycans of cell membrane proteins from human hematopoietic cell lines reveals distinctions in their pattern
- The structure of the extracellular teichoic acids from the allergy-protective bacterium Lactococcus lactis G121
- Aminoquinolines as fluorescent labels for hydrophilic interaction liquid chromatography of oligosaccharides
- Fungal α-arabinofuranosidases of glycosyl hydrolase families 51 and 54 show a dual arabinofuranosyl- and galactofuranosyl-hydrolyzing activity
- Glycoengineering the N-acyl side chain of sialic acid of human erythropoietin affects its resistance to sialidase
- Shiga toxin glycosphingolipid receptor expression and toxin susceptibility of human pancreatic ductal adenocarcinomas of differing origin and differentiation
- Review
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- Research Articles/Short Communications
- Membranes, Lipids, Glycobiology
- Anticancer peptide NK-2 targets cell surface sulphated glycans rather than sialic acids
- Proteolysis
- Extracellular aspartic protease SAP2 of Candida albicans yeast cleaves human kininogens and releases proinflammatory peptides, Met-Lys-bradykinin and des-Arg9-Met-Lys-bradykinin
Articles in the same Issue
- Masthead
- Masthead
- Guest Editorial
- Highlight: Dolce vita – a sample from European glycobiology
- Highlight: Dolce Vita – A Sample From European Glycobiology
- Complicated N-linked glycans in simple organisms
- Methylation – an uncommon modification of glycans
- Protein O-glycosylation analysis
- A sensitive gel-based global O-glycomics approach reveals high levels of mannosyl glycans in the high mass region of the mouse brain proteome
- Glycan profiles of the 27 N-glycosylation sites of the HIV envelope protein CN54gp140
- The analysis of N-glycans of cell membrane proteins from human hematopoietic cell lines reveals distinctions in their pattern
- The structure of the extracellular teichoic acids from the allergy-protective bacterium Lactococcus lactis G121
- Aminoquinolines as fluorescent labels for hydrophilic interaction liquid chromatography of oligosaccharides
- Fungal α-arabinofuranosidases of glycosyl hydrolase families 51 and 54 show a dual arabinofuranosyl- and galactofuranosyl-hydrolyzing activity
- Glycoengineering the N-acyl side chain of sialic acid of human erythropoietin affects its resistance to sialidase
- Shiga toxin glycosphingolipid receptor expression and toxin susceptibility of human pancreatic ductal adenocarcinomas of differing origin and differentiation
- Review
- Structural determinants of the eosinophil cationic protein antimicrobial activity
- Research Articles/Short Communications
- Membranes, Lipids, Glycobiology
- Anticancer peptide NK-2 targets cell surface sulphated glycans rather than sialic acids
- Proteolysis
- Extracellular aspartic protease SAP2 of Candida albicans yeast cleaves human kininogens and releases proinflammatory peptides, Met-Lys-bradykinin and des-Arg9-Met-Lys-bradykinin
