Swimming exercise improves SIRT1, NF-κB, and IL–1β protein levels and pancreatic tissue injury in ovariectomized diabetic rats

Parisa Habibi; Nasser Ahmadiasl; Alireza Nourazarian; Hadi Yousefi

doi:10.1515/hmbci-2021-0069

Article

Swimming exercise improves SIRT1, NF-κB, and IL–1β protein levels and pancreatic tissue injury in ovariectomized diabetic rats

Parisa Habibi , Nasser Ahmadiasl , Alireza Nourazarian and Hadi Yousefi

Published/Copyright: April 7, 2022

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From the journal Hormone Molecular Biology and Clinical Investigation Volume 43 Issue 3

Abstract

Objectives

In this study, we investigated the beneficial effects of swimming exercise on the SIRT1, NF-κB, IL-1β protein levels, and pancreatic tissue damage in an ovariectomized diabetic rat model based on the anti-inflammatory effect of exercise.

Methods

Forty mature female Wistar rats were purchased and divided into sham (n=10) and OVX (bilateral ovariectomy) (n=30) groups. The ovariectomized rats were divided into 1-OVX, 2-ovariectomized diabetic (OVX.D), 3-OVX.D + exercise (OVX.D. E). After surgical recovery, animals in the diabetic group received a high-fat diet for one month. Swimming exercise (1 h/day) was performed concurrently with the start of the HFD diet for eight weeks. At the end of the high-fat diet, streptozotocin (30 mg/kg) was injected intraperitoneally. At the end of the second month, pancreatic tissue was collected from the animals after deep anesthesia for molecular evaluation and histology by Western blotting and hematoxylin-eosin, respectively.

Results

Swimming exercise significantly decreased inflammatory cytokines and tissue damage, and this decrease in cytokine expression appears to be associated with SIRT1 expression. The increase in SIRT1 by training was associated with decreased NF-κB-p65 and IL–1β expression and preventing tissue damage. Induction of diabetes in the ovariectomized group (OVX.D) resulted in a significant increase in NF-κB-p65 and IL–1β proteins and a decrease in the expression of SIRT1 compared with the sham group. However, swimming training significantly reversed these effects compared with the OVX.D group.

Conclusions

Increased inflammation of β-cells impairs insulin secretion in estrogen insufficiency. Swimming exercise eliminates inflammation in post-menopausal diabetes and supports the potential to prevent pancreatic activity after menopause.

Highlights

Combined effects of type 2 diabetes mellitus and estrogen deficiency increased the pancreatic expression of Nf-kB and IL-1B and subsequently decreased SIRT1 and finally lead to tissue injury in the pancreas compared to sham or OVX groups.

The exercise pretreatment significantly decreased destructive effect removing ovary as well as injection of STZ.
The exercise effectively decreased the pancreatic expression of Nf-κB and IL-1B and subsequently increased the pancreatic expression of SIRT1 and led to tissue damage in the pancreas of ovariectomized diabetic rat.
Lipid profile and Blood levels of insulin, glucose and HbA_1c in the exercise group was lower than the OVX and OVX-diabetic groups.

In conclusion, increased β-cell inflammation led to reduced insulin secretion and glucose deregulation in estrogen insufficiency and menopause. Swimming exercise reduced the inflammation of contracting diabetes in the postmenopausal state. The results of this study support the potential preventing effect of exercise from pancreatic injury after surgical menopause.

Keywords: diabetes; inflammation; ovariectomy; pancreas; swimming

Corresponding author: Hadi Yousefi, Department of Basic Medical Sciences, Khoy University of Medical Sciences, Khoy, Iran, E-mail: yousefi_h@khoyums.ac.ir

Funding source: Drug Applied Research Center, Tabriz University of Medical Sciences

Award Identifier / Grant number: 93/5-4/12

Research funding: The Drug Applied Research Center, Tabriz University of Medical Sciences, supported this research with the grant of this study (grant number 93/5-4/12).
Author contributions: HU and NA designed study and experiment; HU and PH carried out the experiments; HU and PH wrote the manuscript; AN edited of the manuscript.
Competing interests: Authors state no conflict of interest.
Ethics approval and consent to participate: All experimental procedures with rats were performed according to the Guidelines for the Care and Use of Animals approved by the Ethics Committee of Tabriz University of Medical Sciences and followed the National Institutes of Health Guide for the Care and Use of Laboratory Animals.

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Received: 2021-08-02

Accepted: 2022-03-12

Published Online: 2022-04-07

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Articles in the same Issue

https://doi.org/10.1515/hmbci-2021-0069

Keywords for this article

diabetes; inflammation; ovariectomy; pancreas; swimming