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Effect of different dietary fats on inflammation and glucose intolerance in high fructose and high fat fed experimental animals

  • Prasad Smvk , Srinivasulu Kommu , Dinesh Yadav , Suresh Kondeti , Rajender Rao Kalashikam and Saravanan Natarajan ORCID logo EMAIL logo
Published/Copyright: March 31, 2022
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Hormone Molecular Biology and Clinical Investigation
From the journal Hormone Molecular Biology and Clinical Investigation Volume 43 Issue 3

Abstract

Objectives

Diet is the major modifiable risk factor for the onset of insulin resistance and its progression into diabetes. In the present study the effect of various dietary fats on inflammatory homeostasis and glucose tolerance is investigated in high fat and high fructose fed mice model.

Methods

C57/BL6J mice were divided into four groups and fed a casein-based diet containing high fructose (45%) and high fat (24%) (clarified butter oil [CBO]; safflower oil [SFFO] and lard oil [LO]) for 120 days; oral glucose tolerance (OGTT), plasma lipid profile and plasma & adipose tissue cytokines levels were compared with the control diet (10% groundnut oil and 59.5% starch) fed animals.

Results

The total cholesterol and triglycerides were higher in CBO and LO fed animals with glucose intolerance and increased body weights; liver and white adipose tissue weights were higher in CBO and LO fed animals respectively. CBO feeding increased the plasma (IFN-γ) and adipose tissue cytokines (IFN-γ, IL-10, IL-6 & TNF-α). LO feeding increased plasma IFN-γ, TNF-α and IL-1β and adipose tissue IL-6. SFFO feeding decreased body weight and tissue cytokines and increased plasma IFN-γ levels without causing impairment in the glucose tolerance.

Conclusions

Consumption of a high fructose and high fat diet which mimic the present-day dietary pattern resulted in altered inflammatory homeostasis and impairment in glucose tolerance in 24% CBO and LO fed animals. The deleterious effects of high fructose feeding were reversed in SFFO fed mice possibly due to the presence of oleic and linoleic acids.

Keywords: cytokines; dietary fatty acids; glucose intolerance; high fructose and high fat diet; inflammation
Corresponding author: Saravanan Natarajan, Scientist D, Department of Biochemistry, ICMR-National Institute for Research in Tuberculosis, Chennai, 600 031, India, Phone: +9140 2836 9676, Fax: +9140 2836 2525, E-mail:
Rajender Rao Kalashikam and Saravanan Natarajan contributed equally to this work.

Funding source: Department of Biotechnology, Ministry Science and Technology, Government of India

Award Identifier / Grant number: PR6197/FNS/20/632/2012

Acknowledgments

The authors acknowledge the support rendered by Directors of ICMR-National Institute of Nutrition & ICMR-National Institute for Research in Tuberculosis.

  1. Research funding: The research work is carried out with the extramural support received from Department of Biotechnology, Ministry Science and Technology, Government of India (PR6197/FNS/20/632/2012).

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: An animal experiment was conducted using C57/BL6 mice and the experimentation procedure was approved by Institutional Animal Ethics Committee, National Centre for Laboratory Animal Science (NCLAS), ICMR-National Institute of Nutrition (ICMR-NIN), Hyderabad, India (P12F/IAEC/NIN/6/2013/NS/C57-M56).

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Received: 2021-07-16
Accepted: 2022-03-12
Published Online: 2022-03-31

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hmbci-2021-0061
Keywords for this article
cytokines; dietary fatty acids; glucose intolerance; high fructose and high fat diet; inflammation

Articles in the same Issue

  1. Frontmatter
  2. Letter to the Editor
  3. Continuing medical education in Covid-19 pandemic
  4. Original Articles
  5. Reduced irisin levels in patients with acromegaly
  6. Clinical investigation of lipoprotein (a) levels in type 2 diabetics for cardiovascular diseases prediction and prognosis
  7. Exploring the relationship between vitamin D and leptin hormones in type 2 diabetes mellitus patients from Kuwait
  8. Serum 25(OH) vitamin D level in Relapsing-Remitting Multiple Sclerosis and clinically isolated syndrome groups
  9. Hypoglycemic, hypolipidemic and hepatoprotective effects of Alpinia officinarum on nicotinamide/streptozotocin induced type II diabetic rats
  10. Association of obesity in T2DM with differential polymorphism of ghrelin, growth hormone secretagogue receptor-1 and telomeres maintenance genes
  11. Effect of different dietary fats on inflammation and glucose intolerance in high fructose and high fat fed experimental animals
  12. Biological reference interval of nitric oxide in health and disease
  13. Maternal hypothyroidism prolongs gestation period and impairs glucose tolerance in offspring of Wistar rats
  14. A study on serum homocysteine and oxidized LDL as markers of cardiovascular risk in patients with overt hypothyroidism
  15. The study of serum hsCRP, ferritin, IL-6 and plasma D-dimer in COVID-19: a retrospective study
  16. Swimming exercise improves SIRT1, NF-κB, and IL–1β protein levels and pancreatic tissue injury in ovariectomized diabetic rats
  17. Short Communications
  18. The second wave of COVID-19 results in outbreak of mucormycosis: diabetes and immunological perspective
  19. A study on lipoprotein-a and PAI-1 in women with polycystic ovary syndrome
  20. Review Articles
  21. Oxytocin, the panacea for long-COVID? a review
  22. COVID-19 infection in pregnancy: a review of existing knowledge
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