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Structural comparison of different isolated eucalyptus lignins and analysis of their interaction mechanism with bovine serum albumin solution under QCM-D

  • Haonan Zhang ORCID logo , Qi Liu , Yanchen Zhu , Zhang Feng , Hao Ren EMAIL logo and Huamin Zhai
Published/Copyright: April 17, 2023
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Holzforschung
From the journal Holzforschung Volume 77 Issue 6

Abstract

In this study, representative lignin samples differing in hydroxyl content, molecular weight, linkage composition and lignin units ratio were prepared from eucalyptus wood by different isolation means and modification means: milled wood lignin (MWL), kraft lignin classified at different pH (KL), lignocresol (LC), lignoresorcinol (LR), lignopyrogallol (LP) as well as lignocresol secondary functional switched samples (LC 2ndderiv). The structure of various lignins was characterized in detail by Fourier transform infrared (FT-IR) spectroscopy, gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). In order to eliminate the interference of other factors, bovine serum albumin (BSA) with relatively simple protein structure and different kinds of lignin and their derivatives were selected as the research objects. The adsorption/desorption behavior and mechanism between lignin samples and BSA were studied by quartz crystal microbalance with dissipation monitoring (QCM-D). The results showed that the content of phenolic hydroxyl was the main factor affecting the interaction of lignin and BSA, but in addition, the molecular size, linkages composition and the types of lignin units also had important effects on its adsorption behavior. The research will provide theoretical guidance and all-round reliable basic data for the interaction of lignin on lignocellulose hydrolysis enzymes in industrial application.

Keywords: adsorption mechanism; bovine serum albumin; eucalyptus lignin; quartz crystal microbalance
Corresponding author: Hao Ren, Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu Province 210037, China, E-mail:
Haonan Zhang and Qi Liu are joint first authors.

Funding source: National Key Research and Development Program of China

Award Identifier / Grant number: 202010298105Y

Funding source: Foundation of State Key Laboratory of Biobased Material and Green Papermaking

Award Identifier / Grant number: KF 201803

  1. Author contributions: Haonan Zhang and Qi Liu: methodology, investigation, writing – original draft, writing – review & editing. Yanchen Zhu: writing & editing. Zhang Feng: investigation. Hao Ren: resources, writing – review & editing, supervision. Huamin Zhai: resources, writing – review & editing.

  2. Research funding: This work was financially supported by the National Key Research and Development Program of China (no. 202010298105Y), and Foundation of State Key Laboratory of Biobased Material and Green Papermaking (no. KF 201803).

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/hf-2022-0170).

Received: 2022-11-05
Accepted: 2023-03-23
Published Online: 2023-04-17
Published in Print: 2023-06-27

© 2023 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Wood Physics/Mechanical Properties
  3. Effects of seismic strain rates on the perpendicular-to-grain compression behaviour of Dahurian larch, Mongolian pine and Chinese poplar: tests and stress-strain model
  4. The effect of the growth ring orientation on spring-back and set-recovery in surface-densified wood
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https://doi.org/10.1515/hf-2022-0170
Keywords for this article
adsorption mechanism; bovine serum albumin; eucalyptus lignin; quartz crystal microbalance

Articles in the same Issue

  1. Frontmatter
  2. Wood Physics/Mechanical Properties
  3. Effects of seismic strain rates on the perpendicular-to-grain compression behaviour of Dahurian larch, Mongolian pine and Chinese poplar: tests and stress-strain model
  4. The effect of the growth ring orientation on spring-back and set-recovery in surface-densified wood
  5. Synergistic improvement to dimensional stability of Populus cathay ana via hemicellulose removal/alkali lignin impregnation
  6. Adding gaseous ammonia with heat treatment to improve the mechanical properties of spruce wood
  7. Wood as a hydrothermally stimulated shape-memory material: mechanisms of shape-memory effect and molecular assembly structure networks
  8. Wood Chemistry
  9. Structural comparison of different isolated eucalyptus lignins and analysis of their interaction mechanism with bovine serum albumin solution under QCM-D
  10. Role of α-Fe2O3 nano-particles in protecting wood from ultraviolet light degradation
  11. Wood Science – Non-Tree Plants
  12. Radial distribution of vascular bundle morphology in Chinese bamboos: machine learning methodology for rapid sampling and classification
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