Ligninases remove phenolic inhibitors and facilitate yeast growth in lignocellulosic hydrolysate
-
Honglei Chen
,
Xin Zhao
Abstract
Sugar-containing hydrolysates as byproducts of dissolving pulp production could be a source of alcohol production, but the presence of fermentation inhibitors hampers this utilization route. In the present study, nine types of phenolic inhibitors were found and their removal by ligninases gave satisfactory results. Laccase removed 73.1% of phenolic inhibitors and produced three new aromatic compounds, whereas the combination of laccase and lignin peroxidase (as a dual-enzyme treatment) resulted in a removal rate of 91.7% without new product formation. Xylose as the main sugar in the hydrolysate was retained with a 90% yield, regardless of the enzymatic treatment, which modified, however, the kinetics of yeast growth. Laccase improved cells’ maximum specific growth rate (vmax) from 0.115 to 0.154 and reduced the saturation constant (Ks) from 53.4 to 42.5, whereas the dual-enzyme system improved vmax to 0.206 and reduced Ks to 42.44. The greater vmax and the smaller Ks imply a better growth rate, and accordingly, the dual-enzyme system is better suited for removing phenolic inhibitors.
Acknowledgments
We thank Associate Professor Pedram Fatehi for guidance and improvement of the manuscript.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: The present work was financially supported by the National Natural Science Foundation of China (funder id: 10.13039/501100001809, grant nos. 31600472, 31570566, 31500489, 31770626, 31670590), the Natural Science Foundation of Shandong Province (ZR2017LEM009), the Taishan Scholars Program and the Foundation for Outstanding Young Scientist in Shandong Province (BS2015SW011).
Employment or leadership: None declared.
Honorarium: None declared.
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©2019 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Original Articles
- Synchrotron X-ray measurements of cellulose in wood cell wall layers of Pinus densiflora in the transmission and reflectance modes. Part 1: results without loading
- NIR hyperspectral imaging (NIR-HI) and μXRD for determination of the transition between juvenile and mature wood of Pinus sylvestris L.
- Characterization of the pits in parenchyma cells of the moso bamboo [Phyllostachys edulis (Carr.) J. Houz.] culm
- Identification of chemical constituents from the bark of Larix kaempferi and their tyrosinase inhibitory effect
- Oxygen delignification: laboratory evaluation of the impact of dissolved organic matter, sodium carbonate and sodium thiosulfate
- A study of thermo-hydro-treated (THT) birch wood by chemical analysis and Py-GC/MS
- Thermally modified (TM) beech wood: compression properties, fracture toughness and cohesive law in mode II obtained from the three-point end-notched flexure (3ENF) test
- Cellulolytic activity of brown-rot Antrodia sinuosa at the initial stage of cellulose degradation
- Ligninases remove phenolic inhibitors and facilitate yeast growth in lignocellulosic hydrolysate
- Development of a reinforced styrene-free unsaturated polyester composite based on bamboo fibers
- Functionalisation of poly(ethylene terephthalate) (PET) surfaces with two cationised xylans by means of two anchoring polymers
Articles in the same Issue
- Frontmatter
- Original Articles
- Synchrotron X-ray measurements of cellulose in wood cell wall layers of Pinus densiflora in the transmission and reflectance modes. Part 1: results without loading
- NIR hyperspectral imaging (NIR-HI) and μXRD for determination of the transition between juvenile and mature wood of Pinus sylvestris L.
- Characterization of the pits in parenchyma cells of the moso bamboo [Phyllostachys edulis (Carr.) J. Houz.] culm
- Identification of chemical constituents from the bark of Larix kaempferi and their tyrosinase inhibitory effect
- Oxygen delignification: laboratory evaluation of the impact of dissolved organic matter, sodium carbonate and sodium thiosulfate
- A study of thermo-hydro-treated (THT) birch wood by chemical analysis and Py-GC/MS
- Thermally modified (TM) beech wood: compression properties, fracture toughness and cohesive law in mode II obtained from the three-point end-notched flexure (3ENF) test
- Cellulolytic activity of brown-rot Antrodia sinuosa at the initial stage of cellulose degradation
- Ligninases remove phenolic inhibitors and facilitate yeast growth in lignocellulosic hydrolysate
- Development of a reinforced styrene-free unsaturated polyester composite based on bamboo fibers
- Functionalisation of poly(ethylene terephthalate) (PET) surfaces with two cationised xylans by means of two anchoring polymers
