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Novel protein-repellent and antimicrobial polysaccharide multilayer thin films

  • Matea Korica , Lidija Fras Zemljič , Matej Bračič , Rupert Kargl , Stefan Spirk , David Reishofer , Katarina Mihajlovski and Mirjana Kostić ORCID logo EMAIL logo
Published/Copyright: October 17, 2018
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Holzforschung
From the journal Holzforschung Volume 73 Issue 1

Abstract

Nanostructured and bio-active polysaccharide-based thin films were manufactured by means of subsequent spin-coated deposition of a regenerated cellulose (RC) layer and a 2,2,6,6-Tetramethylpiperidine-1-oxyl radical (TEMPO) oxidised cellulose nanofibril (TOCN) layer. The bio-activity of the bilayer was achieved by addition of chitosan (CS). The chitosan was either mixed with the TOCN (TOCN+CS) and deposited on the RC layer by spin-coating, or deposited on the RC and TOCN bilayer by pumping its aqueous solution with various pH over the surface of the bilayer. The water content of the thin films and the CS interactions with the bilayer during deposition were studied in situ by means of a quartz crystal microbalance with dissipation (QCM-D). The pH dependent charging behaviour of the TOCN, TOCN+CS and CS dispersions was evaluated by pH-potentiometric titrations. The surface morphology of the thin films was characterised by atomic force microscopy (AFM). The bio-activity of the thin films was evaluated by studying their protein-repellent properties in situ with a continuous flow of bovine serum albumin (BSA) by means of QCM-D and by evaluating their antibacterial properties in vitro against Staphylococcus aureus and Escherichia coli. These polysaccharide-based thin films are high value-added products because of their multifunctionality, high water absorbance capacity, protein-repellence and antimicrobial activity, and have the potential for medical application as a wound dressing material.

Keywords: antimicrobial properties; chitosan; nanostructured polysaccharide thin films; protein-repellent properties; TEMPO oxidised cellulose nanofibrils

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The authors wish to thank the Ministry of Education, Science and Technological Development of the Republic of Serbia for financial support through the project OI 172029.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/hf-2018-0094).

Received: 2018-04-26
Accepted: 2018-09-27
Published Online: 2018-10-17
Published in Print: 2018-12-19

©2019 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hf-2018-0094
Keywords for this article
antimicrobial properties; chitosan; nanostructured polysaccharide thin films; protein-repellent properties; TEMPO oxidised cellulose nanofibrils

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Advances in biorefinery research
  4. Original Articles
  5. Techno-economic feasibility of bioethanol production via biorefinery of olive tree prunings (OTP): optimization of the pretreatment stage
  6. Designing an olive tree pruning biorefinery for the production of bioethanol, xylitol and antioxidants: a techno-economic assessment
  7. Structural characteristics of lignin in pruning residues of olive tree (Olea europaea L.)
  8. Production of xylooligosaccharides and cellulosic ethanol from steam-exploded barley straw
  9. A commercial laccase-mediator system to delignify and improve saccharification of the fast-growing Paulownia fortunei (Seem.) Hemsl.
  10. Organosolv pretreated beech wood as a substrate for acetone butanol ethanol extractive fermentation
  11. Fine-fibrous cellulose II aerogels of high specific surface from pulp solutions in TBAF·H2O/DMSO
  12. Oil-absorbing porous cellulosic material from sized wood pulp fines
  13. Novel protein-repellent and antimicrobial polysaccharide multilayer thin films
  14. Environmental sustainability assessment of HMF and FDCA production from lignocellulosic biomass through life cycle assessment (LCA)
  15. Review
  16. Lignin-based foams as insulation materials: a review
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