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Organosolv pretreated beech wood as a substrate for acetone butanol ethanol extractive fermentation

  • Helena González-Peñas , Thelmo A. Lú-Chau ORCID logo EMAIL logo , Nicolás Botana , María Teresa Moreira , Juan M. Lema and Gemma Eibes
Published/Copyright: November 8, 2018
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Holzforschung
From the journal Holzforschung Volume 73 Issue 1

Abstract

Acetone-butanol-ethanol (ABE) fermentation has a high potential for industrial application in lignocellulose-based biorefinery. Nevertheless, strong end-product inhibition is a severe limitation of this process, which can be partially solved by in situ product recovery techniques, such as extractive fermentation. In this work, the hydrolyzed cellulose from organosolv pre-treated beech wood (Fagus sylvatica L.) was fermented by Clostridium beijerinckii CECT 508 and compared with synthetic P2 medium in conventional and extractive fermentation processes. The conventional control fermentation yielded 9.12 g l−1 of ABE with C. beijerinckii CECT 508, while ABE overproduction of 40% was observed with cellulose hydrolysate as a substrate. After extractive batch fermentation based on 2-butyl-1-octanol as extractant, the final ABE titer was doubled in the P2 control medium over the conventional batch, with respective increases of 50, 190, and 140% for butanol (B), acetone (A) and ethanol (E). In the case of ABE as a hydrolysate medium, the total production increased by 10% with liquid extraction, while mainly B was responsible for this improvement. The total productivity was nevertheless gradually decreased in extractive fermentation, with a significantly slower production rate and the microbial growth compared to conventional fermentation. Moreover, solvent production and sugar consumption stopped, although the aqueous B concentration was below the inhibition threshold. The mid-term toxicity of the extraction solvent 2-butyl-1-octanol seems responsible for these results and this issue should be considered in future research.

Keywords: ABE fermentation; beech wood; Clostridium beijerinckii; extractive fermentation; organosolv treatment of wood

Acknowledgments

This research was supported by the Spanish Government (Ministry of Economy and Competitiveness) through the ERA-IB2 project 2G-Enzymes (PCIN-2015-031). The authors belong to the Galician Competitive Research Group GRC-ED431C 2017/29 and to the CRETUS Strategic Partnership, (AGRUP2015/02). All these programs are co-funded by FEDER (EU). We thank F.J. Salgado and M. Nogueira from the Department of Biochemistry and Molecular Biology, Biology-Biological Research Centre (CIBUS) at the Universidade de Santiago de Compostela for helping us with the flow cytometry analysis carried out in their laboratory facilities. Authors are grateful to Fraunhofer CBP for provision of beech wood organosolv pulp.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: Ministerio de Economía y Competitividad, Funder Id: 10.13039/501100003329, Grant Number: PCIN-2015-031, Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia, Funder Id: 10.13039/501100008425, Grant Number: AGRUP2015/02 and Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia, Funder Id: 10.13039/501100008425, Grant Number: GRC2013-032.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/hf-2018-0098).

Received: 2018-04-30
Accepted: 2018-09-19
Published Online: 2018-11-08
Published in Print: 2018-12-19

©2019 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Advances in biorefinery research
  4. Original Articles
  5. Techno-economic feasibility of bioethanol production via biorefinery of olive tree prunings (OTP): optimization of the pretreatment stage
  6. Designing an olive tree pruning biorefinery for the production of bioethanol, xylitol and antioxidants: a techno-economic assessment
  7. Structural characteristics of lignin in pruning residues of olive tree (Olea europaea L.)
  8. Production of xylooligosaccharides and cellulosic ethanol from steam-exploded barley straw
  9. A commercial laccase-mediator system to delignify and improve saccharification of the fast-growing Paulownia fortunei (Seem.) Hemsl.
  10. Organosolv pretreated beech wood as a substrate for acetone butanol ethanol extractive fermentation
  11. Fine-fibrous cellulose II aerogels of high specific surface from pulp solutions in TBAF·H2O/DMSO
  12. Oil-absorbing porous cellulosic material from sized wood pulp fines
  13. Novel protein-repellent and antimicrobial polysaccharide multilayer thin films
  14. Environmental sustainability assessment of HMF and FDCA production from lignocellulosic biomass through life cycle assessment (LCA)
  15. Review
  16. Lignin-based foams as insulation materials: a review
https://doi.org/10.1515/hf-2018-0098
Keywords for this article
ABE fermentation; beech wood; Clostridium beijerinckii; extractive fermentation; organosolv treatment of wood

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Advances in biorefinery research
  4. Original Articles
  5. Techno-economic feasibility of bioethanol production via biorefinery of olive tree prunings (OTP): optimization of the pretreatment stage
  6. Designing an olive tree pruning biorefinery for the production of bioethanol, xylitol and antioxidants: a techno-economic assessment
  7. Structural characteristics of lignin in pruning residues of olive tree (Olea europaea L.)
  8. Production of xylooligosaccharides and cellulosic ethanol from steam-exploded barley straw
  9. A commercial laccase-mediator system to delignify and improve saccharification of the fast-growing Paulownia fortunei (Seem.) Hemsl.
  10. Organosolv pretreated beech wood as a substrate for acetone butanol ethanol extractive fermentation
  11. Fine-fibrous cellulose II aerogels of high specific surface from pulp solutions in TBAF·H2O/DMSO
  12. Oil-absorbing porous cellulosic material from sized wood pulp fines
  13. Novel protein-repellent and antimicrobial polysaccharide multilayer thin films
  14. Environmental sustainability assessment of HMF and FDCA production from lignocellulosic biomass through life cycle assessment (LCA)
  15. Review
  16. Lignin-based foams as insulation materials: a review
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