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Hydrolytic and oxidative enzyme production through cultivation of Pleurotus ostreatus on pulp and paper industry wastes

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Published/Copyright: May 22, 2018
Holzforschung
From the journal Holzforschung Volume 72 Issue 9

Abstract

The growth of oyster mushroom (Pleurotus ostreatus) on pulp and paper industry wastes was studied. Specifically, the question was investigated whether solid-state fermentation of P. ostreatus on paper-mill deinking sludge and primary sludge substrates is appropriate for production of enzymes, relevant to the pulp and paper industry. Following fermentation, extracellular protein was extracted and the specific activities of four enzymes were determined, namely, the cellulase, xylanase, lipase and peroxidase. Furthermore, the effects of the pH of the extraction buffer on these enzyme activities were determined, along with the effects of the incubation time. The data show that P. ostreatus can grow on solid wastes from the pulp and paper industry, which could help to minimize the waste volume and to decrease the ecological impact. Furthermore, the solid wastes in focus are good substrates for the production of commercially interesting enzymes.

Keywords: cellulase; enzyme; lipase; paper; paper mill sludge; peroxidase; Pleurotus ostreatus; primary sludge; xylanase

Acknowledgements

The authors would like to thank Dr. Christopher Berrie for critical reading and English language editing of the manuscript. We would like to thank Vipap Videm Krško for cooperation and Erik Birk for help in the experimental work (cultivation).

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This study was carried out in the RDI project Cel. Cycle: “Potential of biomass for development of advanced materials and bio-based products” (contract number: OP20.00365), which is cofinanced by the Ministry of Education, Science and Sport of the Republic of Slovenia, and the European Union, as part of the European Regional Development Fund 2016–2020. Financial support was also provided by the Slovenian Research Agency (grant P1-0207).

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

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Received: 2017-11-07
Accepted: 2018-04-23
Published Online: 2018-05-22
Published in Print: 2018-09-25

©2018 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Extractives of Cercidiphyllum japonicum twigs: isolation and structural elucidation of a new galloylflavonol glycoside, anomeric tannins and flavonoids
  4. Preparation of low-cost carbon fiber precursors from blends of wheat straw lignin and commercial textile-grade polyacrylonitrile (PAN)
  5. Comparison of six WPCs made of organo-montmorillonite-modified fibers of four trees, moso bamboo and wheat straw and poly(lactic acid) (PLA)
  6. Synthesis of phenol formaldehyde (PF) resin for fast manufacturing laminated veneer lumber (LVL)
  7. Reducing formaldehyde emission from wood-based panels by modification of UF and MUF resins with condensates obtained from kiln-drying of wood
  8. Analysis of the hydrolysates from cured and uncured urea-formaldehyde (UF) resins with two F/U mole ratios
  9. Theoretical and experimental considerations on the neutral axis of wood beams with a hole in different locations
  10. Axial tensile properties and flexibility characteristics of elementary units from multidimensional bamboo-based composites: radial and tangential moso bamboo slivers
  11. Optical characteristics of Douglas fir at various densities, grain directions and thicknesses investigated by near-infrared spatially resolved spectroscopy (NIR-SRS)
  12. Effect of thermal modification on the micromorphology of decay of hardwoods and softwoods by the white rot fungus Pycnoporus sanguineus
  13. Short Note
  14. Hydrolytic and oxidative enzyme production through cultivation of Pleurotus ostreatus on pulp and paper industry wastes
https://doi.org/10.1515/hf-2017-0179
Keywords for this article
cellulase; enzyme; lipase; paper; paper mill sludge; peroxidase; Pleurotus ostreatus; primary sludge; xylanase

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Extractives of Cercidiphyllum japonicum twigs: isolation and structural elucidation of a new galloylflavonol glycoside, anomeric tannins and flavonoids
  4. Preparation of low-cost carbon fiber precursors from blends of wheat straw lignin and commercial textile-grade polyacrylonitrile (PAN)
  5. Comparison of six WPCs made of organo-montmorillonite-modified fibers of four trees, moso bamboo and wheat straw and poly(lactic acid) (PLA)
  6. Synthesis of phenol formaldehyde (PF) resin for fast manufacturing laminated veneer lumber (LVL)
  7. Reducing formaldehyde emission from wood-based panels by modification of UF and MUF resins with condensates obtained from kiln-drying of wood
  8. Analysis of the hydrolysates from cured and uncured urea-formaldehyde (UF) resins with two F/U mole ratios
  9. Theoretical and experimental considerations on the neutral axis of wood beams with a hole in different locations
  10. Axial tensile properties and flexibility characteristics of elementary units from multidimensional bamboo-based composites: radial and tangential moso bamboo slivers
  11. Optical characteristics of Douglas fir at various densities, grain directions and thicknesses investigated by near-infrared spatially resolved spectroscopy (NIR-SRS)
  12. Effect of thermal modification on the micromorphology of decay of hardwoods and softwoods by the white rot fungus Pycnoporus sanguineus
  13. Short Note
  14. Hydrolytic and oxidative enzyme production through cultivation of Pleurotus ostreatus on pulp and paper industry wastes
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