Imaging hyphal growth of Physisporinus vitreus in Norway spruce wood by means of confocal laser scanning microscopy (CLSM)
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Mark Schubert
,
Chris Stührk
Abstract
Light microscopy and electron microscopy are the most common methods for analyzing wood-decay fungi. However, the 3D visualization and quantification of the filamentous structure of fungi in wood is difficult to realize by means of these traditional techniques. In the present work, confocal laser scanning microscopy (CLSM) was further developed for the quantitative imaging of the 3D microscopic hyphal growth of Physisporinus vitreus, a versatile fungus for engineering value-added wood products. To this purpose, the fungus was stained with a fluorescent dye Alexa Fluor. The 3D information obtained by CLSM has a high potential as a basis for the development of mathematical models for a more precise observation of the growth behavior of wood-decay fungi.
Acknowledgments
The authors gratefully acknowledge J. Hehl (ETH Light Microscopy Center) for helpful discussions on image processing and express their gratitude to the Swiss National Science Foundation (SNF No. 205321-121701) for its financial support.
References
Claxton, N.S., Fellers, T.J., Davidson, M.W. Laser Scanning Confocal Microscopy. Department of Optical Microscopy and Digital Imaging, National High Magnetic Field Laboratory, Florida State University, 2005, p. 37. Available at: http://www.olympusfluoview.com/theory/LSCMIntro.pdf.Search in Google Scholar
Emmenlauer, M., Ronneberger, O., Ponti, A., Schwarb, P., Griffa, A., Filippi, A., Nitschke, R., Driever, W., Burkhardt, H. (2009) XuvTools: free, fast and reliable stitching of large 3D datasets. J. Microsc. 233:42–60.10.1111/j.1365-2818.2008.03094.xSearch in Google Scholar PubMed
Fuhr, M.J., Schubert, M., Schwarze, F.W.M.R., Herrmann, H.J. (2011) Modelling the hyphal growth of the wood-decay fungus Physisporinus vitreus. Fungal Biol. 115:919–932.10.1016/j.funbio.2011.06.017Search in Google Scholar PubMed
Fuhr, M.J, Stührk, C., Münch, B., Schwarze, F., Schubert, M. (2012) Automated quantification of the impact of the wood-decay fungus Physisporinus vitreus on the cell wall structure of Norway spruce by tomographic microscopy. Wood Sci. Technol. 46:769–779.10.1007/s00226-011-0442-ySearch in Google Scholar
Kim, Y.S., Singh, A.P. (1999) Micromorphological characteristics of compression wood degradation in waterlogged archaeological pine wood. Holzforschung 53:381–385.10.1515/HF.1999.063Search in Google Scholar
Lehringer, C., Koch, G., Adusumalli, R.-B., Mook, W.M., Richter, K., Militz, H. (2011a) Effect of Physisporinus vitreus on wood properties of Norway spruce. Part 1: aspects of delignification and surface hardness. Holzforschung 65:711–719.10.1515/hf.2011.021Search in Google Scholar
Lehringer, C., Saake, B., Živković, V., Richter, K., Militz, H. (2011b) Effect of Physisporinus vitreus on wood properties of Norway spruce. Part 2: aspects of microtensile strength and chemical changes. Holzforschung 65:721–727.10.1515/hf.2011.090Search in Google Scholar
Mannes, D., Marone, F., Lehmann, E., Stampanoni, M., Niemz, P. (2009) Application areas of synchrotron radiation tomographic microscopy for wood research. Wood Sci. Technol. 44:67–84.Search in Google Scholar
Plomley, N.J.B. (1958) Formation of the colony in the fungus Chaetomium. Aust. J. Biol. Sci. 12:53–64.Search in Google Scholar
Schwarze, F.W.M.R. (2007) Wood decay under the microscope. Fungal Biol. Rev. 21:133–170.Search in Google Scholar
Schwarze, F.W.M.R., Landmesser, H. (2000) Preferential degradation of pit membranes within tracheids by the basidiomycete Physisporinus vitreus. Holzforschung 54:461–462.10.1515/HF.2000.077Search in Google Scholar
Schwarze, F., Schubert, M. (2011) Physisporinus vitreus: a versatile white rot fungus for engineering value-added wood products. Appl. Microbiol. Biotechnol. 92:431–440.10.1007/s00253-011-3539-1Search in Google Scholar PubMed
Schwarze, F.W.M.R., Landmesser, H., Zgraggen, B., Heeb, M. (2006) Permeability changes in heartwood of Picea abies and Abies alba induced by incubation with Physisporinus vitreus. Holzforschung 60:450–454.10.1515/HF.2006.071Search in Google Scholar
Speranza, M., Gutiérrez, A., del Río, J.C., Bettucci, L., Martínez, À.T., Martínez, M.J. (2009) Sterols and lignin in Eucalyptus globulus Labill. wood: spatial distribution and fungal removal as revealed by microscopy and chemical analyses. Holzforschung 63:362–370.10.1515/HF.2009.041Search in Google Scholar
Taguchi, A., Murata, K., Nakano, T. (2010) Observation of cell shapes in wood cross-sections during water adsorption by confocal laser-scanning microscopy (CLSM). Holzforschung 64:627–631.10.1515/hf.2010.092Search in Google Scholar
Xiao, Y., Kreber, B., Breuil, C. (1999) Localisation of fungal hyphae in wood using immunofluorescence labelling and confocal laser scanning microscopy. Int. Biodeter. Biodegr. 44:185–190.Search in Google Scholar
Xiao, Y., Wakeling, R.N., Singh, A.P. (2000) Use of confocal microscopy in examining fungi and bacteria in wood. Biofouling 15:231–239.10.1080/08927010009386313Search in Google Scholar PubMed
© 2014 by De Gruyter
Articles in the same Issue
- Frontmatter
- Original Articles
- Lignin chemistry and topochemistry during kraft delignification of Eucalyptus globulus genotypes with contrasting pulpwood characteristics
- Rapid functionalisation of cellulose-based materials using a mixture containing laccase activated lauryl gallate and sulfonated lignin
- Aggregation of sodium lignosulfonate above a critical temperature
- Impact of iron(II) and oxygen on degradation of oak – modeling of the Vasa wood
- Morphological, thermal, and structural aspects of dried and redispersed nanofibrillated cellulose (NFC)
- Effects of recovered wood on the formaldehyde release of particleboards
- Oxalic acid production and metal removal during fungal degradation of CCA-treated wood in nutrient culture
- Analysis of cold temperature effect on stress wave velocity in green wood
- Finite element modeling and experimental validation of radio frequency heating (RFH) of curved laminated wood-based panels
- Anti-termitic potential of heartwood and bark extract and chemical compounds isolated from Madhuca utilis Ridl. H. J. Lam and Neobalanocarpus heimii King P. S. Ashton
- Effects of thermal modification on wood ultrastructure analyzed with crystallographic texture
- Short Notes
- Potential contribution of anion exclusion to hydroxide penetration in green liquor-modified kraft pulping
- Improving the weathering on larch wood samples by electron beam irradiation (EBI)
- Effect of plasma treatment on cell-wall adhesion of urea-formaldehyde resin revealed by nanoindentation
- Imaging hyphal growth of Physisporinus vitreus in Norway spruce wood by means of confocal laser scanning microscopy (CLSM)
- Meetings
- Meetings
Articles in the same Issue
- Frontmatter
- Original Articles
- Lignin chemistry and topochemistry during kraft delignification of Eucalyptus globulus genotypes with contrasting pulpwood characteristics
- Rapid functionalisation of cellulose-based materials using a mixture containing laccase activated lauryl gallate and sulfonated lignin
- Aggregation of sodium lignosulfonate above a critical temperature
- Impact of iron(II) and oxygen on degradation of oak – modeling of the Vasa wood
- Morphological, thermal, and structural aspects of dried and redispersed nanofibrillated cellulose (NFC)
- Effects of recovered wood on the formaldehyde release of particleboards
- Oxalic acid production and metal removal during fungal degradation of CCA-treated wood in nutrient culture
- Analysis of cold temperature effect on stress wave velocity in green wood
- Finite element modeling and experimental validation of radio frequency heating (RFH) of curved laminated wood-based panels
- Anti-termitic potential of heartwood and bark extract and chemical compounds isolated from Madhuca utilis Ridl. H. J. Lam and Neobalanocarpus heimii King P. S. Ashton
- Effects of thermal modification on wood ultrastructure analyzed with crystallographic texture
- Short Notes
- Potential contribution of anion exclusion to hydroxide penetration in green liquor-modified kraft pulping
- Improving the weathering on larch wood samples by electron beam irradiation (EBI)
- Effect of plasma treatment on cell-wall adhesion of urea-formaldehyde resin revealed by nanoindentation
- Imaging hyphal growth of Physisporinus vitreus in Norway spruce wood by means of confocal laser scanning microscopy (CLSM)
- Meetings
- Meetings
