Home Fractionation and characterization of lignin-carbohydrate complexes (LCCs) of Eucalyptus globulus in residues left after MWL isolation. Part II: Analyses of xylan-lignin fraction (X-L)
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Fractionation and characterization of lignin-carbohydrate complexes (LCCs) of Eucalyptus globulus in residues left after MWL isolation. Part II: Analyses of xylan-lignin fraction (X-L)

  • Yasuyuki Miyagawa EMAIL logo , Hiroshi Kamitakahara and Toshiyuki Takano
Published/Copyright: March 13, 2013
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Abstract

The residual wood meal left after milled wood lignin (MWL) isolation [milled wood residue (MWR)] of 5-year-old Eucalyptus globulus was fractionated to afford a xylan-lignin fraction (X-L) in 2.9% yield (based on MWR) by the method reported previously. X-L was further fractionated with the lignin solvent 1,4-dioxane/water (9:1, v/v) to give a soluble fraction (XL-F1; 24.0%) and an insoluble fraction (XL-F1-residue; 74.6%; both yields based on X-L). XL-F1-residue was further extracted with the good xylan solvent dimethyl sulfoxide and the soluble fraction was termed XL-F2 (43.0%; based on the XL-F1-residue). XL-F1 was mainly composed of lignin with a small amount of xylan and it is similar to purified MWL, whereas XL-F2 was mainly composed of xylan with some amount of lignin and it is similar to a fraction that was prepared by the extraction of crude MWL with acetic acid [lignin-carbohydrate complex (LCC)-AcOH]. The two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectra of XL-F1 and XL-F2 were interpreted that the former has α-ether-type lignin-carbohydrate (LC) linkages and the latter might have LC linkages of the phenyl glycoside type, which are different from those in LCC-AcOH.


Corresponding author: Yasuyuki Miyagawa, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku Kyoto, 606-8502, Japan, Phone: 075-753-6256, Fax: 075-753-6256

The authors gratefully acknowledge Oji Paper for the supply of xylanase.

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Received: 2012-9-14
Accepted: 2012-12-19
Published Online: 2013-03-13
Published in Print: 2013-08-01

©2013 by Walter de Gruyter Berlin Boston

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