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Steam pretreatment for enzymatic hydrolysis of poplar wood: comparison of optimal conditions with and without SO2 impregnation

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Published/Copyright: June 29, 2012
Holzforschung
From the journal Holzforschung Volume 67 Issue 1

Abstract

Steam refining of non-debarked poplar wood with SO2 impregnation prior to steaming was investigated as pretreatment for enzymatic hydrolysis. Pretreatment conditions were varied in the range of 170°C–220°C, 3–30 min and 0.7–2.5% SO2 according to a factorial design. Predicted steaming conditions for highest carbohydrate yields after enzymatic hydrolysis were at 200°C, 15 min, and 2.5% SO2. The yield of glucose and xylose from control tests under these conditions was 43% representing an increase of 9% compared to results of former experiments without SO2 impregnation. Investigations on lignin extracted from the fibers revealed no distinct differences between pretreatment with and without SO2. No sulfonation occurred by the impregnation with SO2. Topochemical analyses of the fibers by cellular UV microspectrophotometry (UMSP) showed an inhomogeneous lignin distribution within the S2 of fibers after pretreatment without SO2 and local depositions of high UV-absorbing substances in the lumina of fibers and parenchyma cells. The lignin distribution of fiber cell walls after pretreatment with SO2 was more homogeneous with a preserved fiber network and only little amounts of deposited phenolic compounds in the lumina. Therefore, it might be concluded that the expulsion of lignin hinders the enzymes in accessing the cellulose.

Keywords: enzymatic hydrolysis; poplar; SO2 impregnation; steam refining; UV microspectrophotometry
Corresponding author Department of Wood Science, University of Hamburg, Leuschnerstr. 91B, 21031 Hamburg, Germany
Received: 2012-5-7
Accepted: 2012-6-5
Published Online: 2012-06-29
Published in Print: 2013-01-01

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/hf-2012-0076
Keywords for this article
enzymatic hydrolysis; poplar; SO2 impregnation; steam refining; UV microspectrophotometry

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Original Papers
  4. Assessment of total phenols and extractives of mahogany wood by near infrared spectroscopy (NIRS)
  5. Steam pretreatment for enzymatic hydrolysis of poplar wood: comparison of optimal conditions with and without SO2 impregnation
  6. Silica content of 36 Brazilian tropical wood species
  7. Dissolution and acetylation of ball-milled lignocellulosic biomass in ionic liquids at room temperature: application to nuclear magnetic resonance analysis of cell-wall components
  8. Effects of hydrothermal processing on the cellulosic fraction of Eucalyptus globulus wood
  9. Wood capillarity and deacetylation during eucalyptus alkaline impregnation. Sulphidity effects and comparison between transverse directions
  10. Effects of climatic test parameters on acetic acid emission from beech (Fagus sylvatica L.)
  11. Rational production of veneer by IR-heating of green wood during peeling: Modeling experiments
  12. Measurement of intra-ring wood density by means of imaging VIS/NIR spectroscopy (hyperspectral imaging)
  13. Coarse graining of wood cell arrangement and density dependence of elasticity
  14. Changes in viscoelastic vibrational properties between compression and normal wood: roles of microfibril angle and of lignin
  15. Elastic properties of cellular wood panels with hexagonal and auxetic cores
  16. Alternative experimental method to evaluate moment-carrying capacity of traditional wood-to-wood joint with small scale experiment
  17. Detection of iron-chelating and iron-reducing compounds in four brown rot fungi
  18. Synthesis and fungicidal activity of dehydroabietyl-1,2,4-triazolo-thiazolidinones
  19. Meetings
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