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Lactobacillus plantarum mitigates sexual-reproductive deficits by modulating insulin receptor expression in the hypothalamic-pituitary-testicular axis of hyperinsulinemic mice

  • Edem Ekpenyong Edem ORCID logo EMAIL logo , Blessing Uyo Nathaniel , Kate Eberechukwu Nebo , Abiola Oluwatosin Obisesan , Ayodeji Augustine Olabiyi , Elizabeth Toyin Akinluyi and Azeez Olakunle Ishola
Published/Copyright: May 18, 2021
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Drug Metabolism and Personalized Therapy
From the journal Drug Metabolism and Personalized Therapy Volume 36 Issue 4

Abstract

Objectives

Hyperinsulinemia increases the risk factor of diabetes and infertility at a manifold. Lactobacillus plantarum has several medical significances with limited reports. Hence, this study assessed the effect of L. plantarum on sexual-reproductive functions and distribution of insulin receptors in the hypothalamic-pituitary-testicular axis of hyperinsulinemic mice.

Methods

Forty male adult mice were divided into five groups as follows: control, high-fat diet (HFD) + streptozotocin (STZ), therapeutic, co-administration group type 1 (CO-AD) and probiotics. They were either simultaneously exposed to an HFD and L. plantarum treatment for 28 days with a dose of STZ injection to induce hyperinsulinemia on day 28 or treated with L. plantarum for 14 days, and following induction of hyperinsulinemia. Mice were subjected to a sexual behavioural test and thereafter sacrificed under euthanasia condition. Blood, brain and testes were collected for biochemical and immunohistochemical assays.

Results

Treatment with L. plantarum ameliorated reproductive hormones activity disruption, sexual behavioural defects, antioxidant imbalance, insulin dysregulation and lipid metabolism dysfunction following exposure to HFD + STZ when compared to the hyperinsulinemic untreated mice.

Conclusions

Taken together, data from this study reveal that L. plantarum abrogated hyperinsulinemia-induced male sexual and reproductive deficits by modulating antioxidant status, lipid metabolism and insulin signalling in the hypothalamic-pituitary-testicular axis of mice.

Keywords: hyperinsulinemia; hypothalamic-pituitary-testicular axis; insulin-like growth factor-1 receptor; Lactobacillus plantarum ; seminal parameters; sexual behaviour
Corresponding author: Edem Ekpenyong Edem, Department of Anatomy, College of Medicine and Health Sciences, Afe Babalola University, P.M.B. 5454, Ado-Ekiti, Ekiti State, Nigeria, Phone: +2347037402143, E-mail:

Acknowldgements

The authors acknowledge the support in form of facilities and materials provided for use in this stud by Afe Babalola University Ado-Ekiti.

  1. Research funding: None declared.

  2. Author contributions: EEE: Conceptualization, methodology, supervision and editing. BUN: Project administration, resources and writing of the original draft. KEN: Project administration and data curation. AOO: Validation, project administration and resources. AAO: Validation, reviewing and editing. AOI and ETA: Resources, data curation and formal analyses. All authors reviewed the manuscript final draft before submission. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Not applicable.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: The animals were used according to the guidelines of the National Council for Animal Experiments Control (CONCEA) and following the National Institute of Health Guide for Care and Use of Laboratory animals [24]. The College Research Ethics Committee of Afe Babalola University approved this study with protocol number AB/EC/19/10/51.

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Received: 2021-01-01
Accepted: 2021-04-05
Published Online: 2021-05-18

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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  2. Review
  3. Oxy+ (arthrospira) and its medicinal importance: an appraisal
  4. Original Articles
  5. In vitro inhibitory effects of glucosamine, chondroitin and diacerein on human hepatic CYP2D6
  6. Efficacy of Majoon-e-Seer Alvi Khan in dyslipidemia: a single blind randomized standard controlled clinical trial
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  8. Evaluation of the need for pharmacogenomics testing among physicians in the West Bank of Palestine
  9. Habitual khat chewing alters urinary inorganic profile in adult healthy males
  10. Trichostatin A sensitizes hepatoma cells to Taxol more than 5-Aza-dC and dexamethasone
  11. Ocimum gratissimum leaf extract ameliorates phenylhydrazine-induced anaemia and toxicity in Wistar rats
  12. Lactobacillus plantarum mitigates sexual-reproductive deficits by modulating insulin receptor expression in the hypothalamic-pituitary-testicular axis of hyperinsulinemic mice
  13. Consumption of Ashtanga Ghrita (clarified cow butter added with herb extracts) improves cognitive dysfunction induced by scopolamine in rats via regulation of acetylcholinesterase activity and oxidative stress
  14. Letter to the Editor
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https://doi.org/10.1515/dmpt-2021-1000195
Keywords for this article
hyperinsulinemia; hypothalamic-pituitary-testicular axis; insulin-like growth factor-1 receptor; Lactobacillus plantarum; seminal parameters; sexual behaviour

Articles in the same Issue

  1. Frontmatter
  2. Review
  3. Oxy+ (arthrospira) and its medicinal importance: an appraisal
  4. Original Articles
  5. In vitro inhibitory effects of glucosamine, chondroitin and diacerein on human hepatic CYP2D6
  6. Efficacy of Majoon-e-Seer Alvi Khan in dyslipidemia: a single blind randomized standard controlled clinical trial
  7. Prevalence of alcohol-drug interactions in community-dwelling older patients with polypharmacy
  8. Evaluation of the need for pharmacogenomics testing among physicians in the West Bank of Palestine
  9. Habitual khat chewing alters urinary inorganic profile in adult healthy males
  10. Trichostatin A sensitizes hepatoma cells to Taxol more than 5-Aza-dC and dexamethasone
  11. Ocimum gratissimum leaf extract ameliorates phenylhydrazine-induced anaemia and toxicity in Wistar rats
  12. Lactobacillus plantarum mitigates sexual-reproductive deficits by modulating insulin receptor expression in the hypothalamic-pituitary-testicular axis of hyperinsulinemic mice
  13. Consumption of Ashtanga Ghrita (clarified cow butter added with herb extracts) improves cognitive dysfunction induced by scopolamine in rats via regulation of acetylcholinesterase activity and oxidative stress
  14. Letter to the Editor
  15. Fatal thiopurine toxicity: pre-emptive testing of NUDT15 415C>T polymorphism may be life saving in South Asian population
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