Abstract
Carbon dots (CDs) are versatile nanomaterials valued for their fluorescence, biocompatibility, and potential in bioimaging and drug delivery. Current synthesis methods, such as hydrothermal or microwave techniques, often require specialized equipment and energy-intensive processes, limiting their accessibility. This study presents an efficient, sustainable approach to synthesizing CDs from watermelon peel, an abundant waste biomass, using a round-bottom flask. Watermelon peel was sun-dried, crushed, and boiled with distilled water (1:10 w/v) for 5 h, followed by centrifugation. The resulting CDs exhibited blue fluorescence under a fluorometer, indicative of small particle sizes (<10 nm), and a UV-vis absorption peak at 250 nm, consistent with π-π* transitions of conjugated C=C and C=O bonds. This method leverages simple equipment and a renewable precursor, offering a cost-effective, eco-friendly alternative to conventional techniques. The CDs’ optical properties suggest suitability for biomedical applications, though further optimization (e.g., quantum yield, size distribution) is needed. This work highlights the potential of waste-derived CDs, contributing to sustainable nanotechnology development.
Acknowledgments
The authors thank Chaitanya Bharathi Institute of Technology (CBIT), Hyderabad, for in-house project financial assistance through the CBIT/PROJ-IH/I023/Chemical/D001/2024 grant.
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Research ethics: Not applicable.
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Informed consent: Not applicable.
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Author contributions: All authors have accepted responsibility for the entire content of the manuscript and approved its submission.
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Use of Large Language Models, AI and Machine Learning Tools: None declared.
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Conflict of interest: The authors state no conflict of interest.
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Research funding: In-house project financial assistance through the CBIT/PROJ-IH/I023/Chemical/D001/2024 grant.
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Data availability: Not applicable.
References
1. Zhu, S, Meng, Q, Wang, L, Zhang, J, Song, Y, Jin, H, et al.. Highly photoluminescent carbon dots for multicolor patterning, sensors, and bioimaging. Angew Chem Int Ed 2013;52:3953–7. https://doi.org/10.1002/anie.201300519.Search in Google Scholar PubMed
2. Cui, L, Ren, X, Sun, M, Liu, H, Xia, L. Carbon dots: synthesis, properties and applications. Nanomaterials 2021;11:3419. https://doi.org/10.3390/nano11123419.Search in Google Scholar PubMed PubMed Central
3. Xu, X, Ray, R, Gu, Y, Ploehn, HJ, Gearheart, L, Raker, K, et al.. Electrophoretic analysis and purification of fluorescent single-walled carbon nanotube fragments. J Am Chem Soc 2004;126:12736–7. https://doi.org/10.1021/ja040082h.Search in Google Scholar PubMed
4. Sahu, S, Behera, B, Maiti, TK, Mohapatra, S. Simple one-step synthesis of highly luminescent carbon dots from orange juice: application as excellent bio-imaging agents. Chem Commun 2012;48:8835. https://doi.org/10.1039/c2cc33796g.Search in Google Scholar PubMed
5. Song, Y, Zhu, S, Yang, B. Bioimaging based on fluorescent carbon dots. RSC Adv 2014;4:27184. https://doi.org/10.1039/c3ra47994c.Search in Google Scholar
6. Baker, SN, Baker, GA. Luminescent carbon nanodots: emergent nanolights. Angew Chem Int Ed 2010;49:6726–44. https://doi.org/10.1002/anie.200906623.Search in Google Scholar PubMed
7. Zhuo, Y, Miao, H, Zhong, D, Zhu, S, Yang, X. One-step synthesis of high quantum-yield and excitation-independent emission carbon dots for cell imaging. Mater Lett 2015;139:197–200. https://doi.org/10.1016/j.matlet.2014.10.048.Search in Google Scholar
8. Sinha, R, Roy, N, Rajasekhar, R, Karnawat, A, Mandal, TK. N-doped carbon dot from cigarette-tobacco: picric acid sensing in real water sample and synthesis of CD-MWCNT nano-composite for UV-photodetection. J Environ Chem Eng 2021;9:104971. https://doi.org/10.1016/j.jece.2020.104971.Search in Google Scholar
9. Lu, W, Qin, X, Liu, S, Chang, G, Zhang, Y, Luo, Y, et al.. Economical, green synthesis of fluorescent carbon nanoparticles and their use as probes for sensitive and selective detection of Mercury(II) ions. Anal Chem 2012;84:5351–7. https://doi.org/10.1021/ac3007939.Search in Google Scholar PubMed
10. Pal, T, Mohiyuddin, S, Packirisamy, G. Facile and green synthesis of multicolor fluorescence carbon dots from curcumin: in vitro and In vivo bioimaging and other applications. ACS Omega 2018;3:831–43. https://doi.org/10.1021/acsomega.7b01323.Search in Google Scholar PubMed PubMed Central
11. De Andrés, F, Ríos, Á. Carbon dots – Separative techniques: tools-objective towards green analytical nanometrology focused on bioanalysis. Microchem J 2021;161:105773. https://doi.org/10.1016/j.microc.2020.105773.Search in Google Scholar
12. Li, L, Zhu, X. Enhanced photocatalytic hydrogen evolution of carbon quantum dot modified 1D protonated nanorods of graphitic carbon nitride. ACS Appl Nano Mater 2018;1:5337–44. https://doi.org/10.1021/acsanm.8b01381.Search in Google Scholar
13. Zhou, J, Sheng, Z, Han, H, Zou, M, Li, C. Facile synthesis of fluorescent carbon dots using watermelon peel as a carbon source. Mater Lett 2012;66:222–4. https://doi.org/10.1016/j.matlet.2011.08.081.Search in Google Scholar
14. Wang, B, Yang, P, Zhou, G, Shi, Q, Xie, X. Low temperature synthesis of yellow-green emission carbon dots with excellent antibacterial activity for Escherichia coli and Methicillin-resistant Staphylococcus aureus. Surf Interfaces 2025;72:107056. https://doi.org/10.1016/j.surfin.2025.107056.Search in Google Scholar
15. Wu, JY, Huang, YC. Low-energy-consumption rapid synthesis of carbon dots at room temperature from combusted food waste with versatile analytical applications. Food Chem 2024;446:138908. https://doi.org/10.1016/j.foodchem.2024.138908.Search in Google Scholar PubMed
16. Das, R, Parveen, S, Bora, A, Giri, PK. Origin of high photoluminescence yield and high SERS sensitivity of nitrogen-doped graphene quantum dots. Carbon 2020;160:273–86. https://doi.org/10.1016/j.carbon.2020.01.030.Search in Google Scholar
17. Majumder, T, Debnath, K, Dhar, S, Hmar, JJL, Mondal, SP. Nitrogen‐doped graphene quantum dot‐decorated ZnO nanorods for improved electrochemical solar energy conversion. Energy Tech 2016;4:950–8. https://doi.org/10.1002/ente.201600007.Search in Google Scholar
18. Fan, YZ, Zhang, Y, Li, N, Liu, SG, Liu, T, Li, NB, et al.. A facile synthesis of water-soluble carbon dots as a label-free fluorescent probe for rapid, selective and sensitive detection of picric acid. Sensor Actuator B Chem 2017;240:949–55. https://doi.org/10.1016/j.snb.2016.09.063.Search in Google Scholar
19. Wang, Y, Chang, X, Jing, N, Zhang, Y. Hydrothermal synthesis of carbon quantum dots as fluorescent probes for the sensitive and rapid detection of picric acid. Anal Methods 2018;10:2775–84. https://doi.org/10.1039/C8AY00441B.Search in Google Scholar
20. Sun, Y-P, Zhou, B, Lin, Y, Wang, W, Fernando, KAS, Pathak, P, et al.. Quantum-sized carbon dots for bright and colorful photoluminescence. J Am Chem Soc 2006;128:7756–7. https://doi.org/10.1021/ja062677d.Search in Google Scholar PubMed
21. Atchudan, R, Edison, TNJI, Aseer, KR, Perumal, S, Karthik, N, Lee, YR. Highly fluorescent nitrogen-doped carbon dots derived from Phyllanthus acidus utilized as a fluorescent probe for label-free selective detection of Fe3+ ions, live cell imaging and fluorescent ink. Biosens Bioelectron 2018;99:303–11. https://doi.org/10.1016/j.bios.2017.07.076.Search in Google Scholar PubMed
22. Chen, S, Song, Y, Shi, F, Liu, Y, Ma, Q. Sensitive detection of picric acid based on creatinine-capped solid film assembled by nitrogen-doped graphene quantum dots and chitosan. Sensor Actuator B Chem 2016;231:634–40. https://doi.org/10.1016/j.snb.2016.03.071.Search in Google Scholar
23. Khan, ZMSH, Saifi, S, Shumaila, AZ, Khan, SA, Zulfequar, M. A facile one step hydrothermal synthesis of carbon quantum dots for label -free fluorescence sensing approach to detect picric acid in aqueous solution. J Photochem Photobiol Chem 2020;388:112201. https://doi.org/10.1016/j.jphotochem.2019.112201.Search in Google Scholar
24. Li, J, Zhang, L, Li, P, Zhang, Y, Dong, C. One step hydrothermal synthesis of carbon nanodots to realize the fluorescence detection of picric acid in real samples. Sensor Actuator B Chem 2018;258:580–8. https://doi.org/10.1016/j.snb.2017.11.096.Search in Google Scholar
25. Li, N, Liu, SG, Fan, YZ, Ju, YJ, Xiao, N, Luo, HQ, et al.. Adenosine-derived doped carbon dots: from an insight into effect of N/P co-doping on emission to highly sensitive picric acid sensing. Anal Chim Acta 2018;1013:63–70. https://doi.org/10.1016/j.aca.2018.01.049.Search in Google Scholar PubMed
26. Dhall, S, Jaggi, N. Improvement in structural and electrical properties of cuprous oxide-coated multiwalled carbon nanotubes. Bull Mater Sci 2014;37:1427–31. https://doi.org/10.1007/s12034-014-0092-4.Search in Google Scholar
27. Das, R, Rajender, G, Giri, PK. Anomalous fluorescence enhancement and fluorescence quenching of graphene quantum dots by single walled carbon nanotubes. Phys Chem Chem Phys 2018;20:4527–37. https://doi.org/10.1039/C7CP06994D.Search in Google Scholar PubMed
28. Varghese, OK, Kichambre, PD, Gong, D, Ong, KG, Dickey, EC, Grimes, CA. Gas sensing characteristics of multi-wall carbon nanotubes. Sensor Actuator B Chem 2001;81:32–41. https://doi.org/10.1016/S0925-4005(01)00923-6.Search in Google Scholar
29. Anastas, PT, Warner, JC. Green chemistry: theory and practice. Oxford: Oxford University Press; 2000.10.1093/oso/9780198506980.001.0001Search in Google Scholar
30. Sinha, R, Bidkar, AP, Rajasekhar, R, Ghosh, SS, Mandal, TK. A facile synthesis of nontoxic luminescent carbon dots for detection of chromium and iron in real water sample and bio‐imaging. Can J Chem Eng 2020;98:194–204. https://doi.org/10.1002/cjce.23630.Search in Google Scholar
31. Lim, SY, Shen, W, Gao, Z. Carbon quantum dots and their applications. Chem Soc Rev 2015;44:362–81. https://doi.org/10.1039/c4cs00269e.Search in Google Scholar PubMed
32. Li, H, Kang, Z, Liu, Y, Lee, S-T. Carbon nanodots: synthesis, properties and applications. J Mater Chem 2012;22:24230. https://doi.org/10.1039/c2jm34690g.Search in Google Scholar
33. Sharma, A, Das, J. Small molecules derived carbon dots: synthesis and applications in sensing, catalysis, imaging, and biomedicine. J Nanobiotechnol 2019;17. https://doi.org/10.1186/s12951-019-0525-8.Search in Google Scholar PubMed PubMed Central
34. Dong, Y, Wang, R, Li, H, Shao, J, Chi, Y, Lin, X, et al.. Polyamine-functionalized carbon quantum dots for chemical sensing. Carbon 2012;50:2810–5. https://doi.org/10.1016/j.carbon.2012.02.046.Search in Google Scholar
35. Shabbir, H, Csapó, E, Wojnicki, M. Carbon quantum dots: the role of surface functional groups and proposed mechanisms for metal ion sensing. INORGA 2023;11:262. https://doi.org/10.3390/inorganics11060262.Search in Google Scholar
36. George, E, Hanan, A, Mustafa, MN, Walvekar, R, Mathkor, DM, Haque, S, et al.. Carbon dot-based biosensors for continuous glucose monitoring in point-of-care devices: advancements, challenges, and future prospects. J Electrochem Soc 2025;172:047508. https://doi.org/10.1149/1945-7111/adc8d7.Search in Google Scholar
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