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Clinical pilot study on microfluidic automation of IGH-VJ library preparation for next generation sequencing

  • Jacob F. Hess ORCID logo EMAIL logo , Michaela Kotrová , Birgit Fricke , Simona Songia , Silvia Rigamonti , Roberta Cavagna , Manuela Tosi , Nils Paust , Anton W. Langerak , Orietta Spinelli , Giovanni Cazzaniga , Monika Brüggemann and Tobias Hutzenlaub
Published/Copyright: December 29, 2023
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 62 Issue 7

Received: 2023-10-02
Accepted: 2023-12-18
Published Online: 2023-12-29
Published in Print: 2024-06-25

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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  1. Frontmatter
  2. Editorial
  3. LC-MS/MS random access automation – a game changer for the 24/7 clinical laboratory
  4. Reviews
  5. Neurofilament light protein as a biomarker for spinal muscular atrophy: a review and reference ranges
  6. Differential diagnosis of ascites: etiologies, ascitic fluid analysis, diagnostic algorithm
  7. Opinion Papers
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  9. Blood over-testing: impact, ethical issues and mitigating actions
  10. General Clinical Chemistry and Laboratory Medicine
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  25. An automatic analysis and quality assurance method for lymphocyte subset identification
  26. Cancer Diagnostics
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  29. Analytical evaluation of the novel Mindray high sensitivity cardiac troponin I immunoassay on CL-1200i
  30. Infectious Diseases
  31. A reactive monocyte subset characterized by low expression of CD91 is expanded during sterile and septic inflammation
  32. Letters to the Editor
  33. Inadvertent omission of a specimen integrity comment – an overlooked post-analytical error
  34. Falsely elevated T3 due to interference of anti-T3 autoantibodies: a case report
  35. Validation of the Siemens Atellica cortisol immunoassay compared to liquid chromatography mass spectrometry in adrenal venous sampling for primary hyperaldosteronism
  36. Lessons learned from site-specific sampling and biological half-life of IGFII and IIE(68-88) peptide: a case study
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  38. Clinical pilot study on microfluidic automation of IGH-VJ library preparation for next generation sequencing
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https://doi.org/10.1515/cclm-2023-1346
Keywords for this article
automation; high-throughput nucleotide sequencing; microfluidics; precursor cell lymphoblastic leukemia-lymphoma

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. LC-MS/MS random access automation – a game changer for the 24/7 clinical laboratory
  4. Reviews
  5. Neurofilament light protein as a biomarker for spinal muscular atrophy: a review and reference ranges
  6. Differential diagnosis of ascites: etiologies, ascitic fluid analysis, diagnostic algorithm
  7. Opinion Papers
  8. Clinical Decision Support System in laboratory medicine
  9. Blood over-testing: impact, ethical issues and mitigating actions
  10. General Clinical Chemistry and Laboratory Medicine
  11. An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure for the quantification of zonisamide in human serum and plasma
  12. An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure for the quantification of carbamazepine in human serum and plasma
  13. An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of phenobarbital in human serum and plasma
  14. An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of primidone in human serum and plasma
  15. An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure for the quantification of carbamazepine-10,11-epoxide in human serum and plasma
  16. Should we depend on reference intervals from manufacturer package inserts? Comparing TSH and FT4 reference intervals from four manufacturers with results from modern indirect methods and the direct method
  17. Comparison of three chatbots as an assistant for problem-solving in clinical laboratory
  18. Evidence-based cutoffs for total and adjusted calcium: a major factor in detecting severe hypo- and hypercalcemia
  19. Minor head injury in anticoagulated patients: performance of biomarkers S100B, NSE, GFAP, UCH-L1 and Alinity TBI in the detection of intracranial injury. A prospective observational study
  20. A comparative evaluation of the analytical performances of premier resolution-high-performance liquid chromatography (PR-HPLC) with capillary zone electrophoresis (CZE) assays for the detection of hemoglobin variants and the quantitation of HbA0, A2, E, and F
  21. Get reliable laboratory findings – how to recognize the deceptive effects of angiotensin-converting enzyme inhibitor therapy in the laboratory diagnostics of sarcoidosis?
  22. Reference Values and Biological Variations
  23. Vitamin D and vitamin K status in postmenopausal women with normal and low bone mineral density
  24. Hematology and Coagulation
  25. An automatic analysis and quality assurance method for lymphocyte subset identification
  26. Cancer Diagnostics
  27. Machine learning-based delta check method for detecting misidentification errors in tumor marker tests
  28. Cardiovascular Diseases
  29. Analytical evaluation of the novel Mindray high sensitivity cardiac troponin I immunoassay on CL-1200i
  30. Infectious Diseases
  31. A reactive monocyte subset characterized by low expression of CD91 is expanded during sterile and septic inflammation
  32. Letters to the Editor
  33. Inadvertent omission of a specimen integrity comment – an overlooked post-analytical error
  34. Falsely elevated T3 due to interference of anti-T3 autoantibodies: a case report
  35. Validation of the Siemens Atellica cortisol immunoassay compared to liquid chromatography mass spectrometry in adrenal venous sampling for primary hyperaldosteronism
  36. Lessons learned from site-specific sampling and biological half-life of IGFII and IIE(68-88) peptide: a case study
  37. The added value of automated HPC count: detecting clinically important interferences on the flow cytometric CD34+ cell count
  38. Clinical pilot study on microfluidic automation of IGH-VJ library preparation for next generation sequencing
  39. Long-term effects of interventions applied to optimize the use of 25-OH vitamin D tests in primary health care
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