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Chinese normotensive and essential hypertensive reference intervals for plasma aldosterone and renin activity by liquid chromatography-tandem mass spectrometry

  • Jenny Yeuk-Ki Cheng EMAIL logo , Felix Chi-Kin Wong , Edith Wing-Kar Chow , Wendy Wan-Hang Lau , Kitty Kit-Ting Cheung , Timothy Hua-Tse Cheng , Teresa Kam-Chi Tsui , Alan Shek-Lun Chan , Clara Wai-Shan Lo and Chung-Shun Ho
Published/Copyright: August 4, 2022
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 60 Issue 10

Abstract

Objectives

The renin-angiotensin-aldosterone system (RAAS) regulates blood pressure. Plasma renin activities (PRA) and plasma aldosterone concentrations (PAC) are biomarkers related to RAAS. Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based measurements for PRA and PAC have become popular. Method-specific reference intervals (RIs) are required.

Methods

Routine PRA and PAC services in a Hong Kong teaching hospital were based on LC-MS/MS methods. PRA and PAC RIs were developed for normotensive subjects and essential hypertensive (EH) patients. Healthy volunteers were recruited to establish normotensive RIs. PRA and PAC results of hypertensive patients with urine aldosterone tests for primary aldosteronism (PA) screening were retrieved from the laboratory information system. Patients without PA were included. Patients with secondary hypertension and patients on medications affecting the RAAS were excluded. The central 95% RIs were established based on the recommendations of the Clinical and Laboratory Standards Institute guideline C28-A3.

Results

PRA and PAC of 170 normotensive volunteers and 362 EH patients were analysed. There was no sex-specific difference in PRA and PAC for normotensive and EH reference subjects. Differences for PRA and PAC were noted between normotensive subjects aged below 45 and their older counterparts. However, such a difference was only identified for PRA but not PAC in EH patients. Age-specific RIs were established accordingly.

Conclusions

This study presented age-specific LC-MS/MS RIs of PRA and PAC for both normotensive and EH populations for local Chinese in Hong Kong.

Keywords: aldosterone; liquid chromatography-tandem mass spectrometry; reference interval; renin
Corresponding author: Jenny Yeuk-Ki Cheng, Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, 30–32 Ngan Shing Street, Statin, NT, Hong Kong, Phone: +(852) 5569 9723, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: The study has been approved by The Chinese University of Hong Kong–New Territories East Cluster Clinical Research Ethics Committee.

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Received: 2022-04-04
Accepted: 2022-07-13
Published Online: 2022-08-04
Published in Print: 2022-09-27

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/cclm-2022-0325
Keywords for this article
aldosterone; liquid chromatography-tandem mass spectrometry; reference interval; renin

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. The relevance of establishing method-dependent decision thresholds of serum folate in pregnancy and lactation: when the laboratory stewardship meets the health-care needs
  4. Reviews
  5. Strategies to minimise the current disadvantages experienced by women in faecal immunochemical test-based colorectal cancer screening
  6. What is the impact of circulating histones in COVID-19: a systematic review
  7. Mini Review
  8. Diagnostic utility of pleural cell-free nucleic acids in undiagnosed pleural effusions
  9. Guidelines and Recommendations
  10. Evaluation of the cardiovascular risk in patients undergoing major non-cardiac surgery: role of cardiac-specific biomarkers
  11. Genetics and Molecular Diagnostics
  12. Detection of DNA copy number alterations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of single nucleotide polymorphisms
  13. General Clinical Chemistry and Laboratory Medicine
  14. Fifty years of newborn screening for congenital hypothyroidism: current status in Australasia and the case for harmonisation
  15. Commutability assessment of reference materials for homocysteine
  16. External quality assessment for detection of methylated Syndecan 2 (SDC2) in China
  17. Patient-based real-time quality control for quantitative hepatitis B virus DNA test using moving rate of positive and negative patient results
  18. Performance of HDL-C measurements assessed by a 4-year trueness-based EQA/PT program in China
  19. Two-site evaluation of the Roche Elecsys Vitamin D total III assay
  20. A novel point-of-care device accurately measures thyrotropin in whole blood, capillary blood and serum
  21. IgA rheumatoid factor in rheumatoid arthritis
  22. Reference Values and Biological Variations
  23. Reference intervals of 14 biochemical markers for children and adolescence in China: the PRINCE study
  24. Chinese normotensive and essential hypertensive reference intervals for plasma aldosterone and renin activity by liquid chromatography-tandem mass spectrometry
  25. Biological variation estimates obtained from Chinese subjects for 32 biochemical measurands in serum
  26. Cancer Diagnostics
  27. Testosterone analysis in castrated prostate cancer patients: suitability of the castration cut-off and analytical accuracy of the present-day clinical immunoassays
  28. Cardiovascular Diseases
  29. Point-of-care high-sensitivity troponin-I analysis in capillary blood for acute coronary syndrome diagnostics
  30. Platelet count and clinical outcomes among ischemic stroke patients with endovascular thrombectomy in DIRECT-MT
  31. Infectious Diseases
  32. Measurement of anti SARS-CoV-2 RBD IgG in saliva: validation of a highly sensitive assay and effects of the sampling collection method and correction by protein
  33. Letters to the Editors
  34. The simple reproducibility of a measurement result does not equal its overall measurement uncertainty
  35. Complexity of serological assays and misunderstandings of WHO International Units
  36. Ct values of different SARS CoV2 variants: a single center observational study from Innsbruck, Austria
  37. Alignment of the new generation of Abbott Alinity γ-glutamyltransferase assay to the IFCC reference measurement system should be improved
  38. Thermal and chronological stability of monocyte distribution width (MDW), the new biomarker for sepsis
  39. Two comments for the current commutability assessment
  40. Plasma neurofilament light chain in Chinese children with later-onset spinal muscular atrophy
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